##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546907_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1651105
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685603883459866	31.0	31.0	34.0	30.0	34.0
2	32.016815405440596	31.0	31.0	34.0	30.0	34.0
3	31.84044806356955	31.0	31.0	34.0	30.0	34.0
4	35.71613919163227	37.0	35.0	37.0	35.0	37.0
5	35.767014211694594	37.0	35.0	37.0	35.0	37.0
6	35.83537509728333	37.0	35.0	37.0	35.0	37.0
7	36.11100444853598	37.0	35.0	37.0	35.0	37.0
8	13.30978829329449	0.0	0.0	36.0	0.0	37.0
9	24.965751421018044	18.0	17.0	39.0	17.0	39.0
10	32.71044361200529	32.0	32.0	38.0	27.0	39.0
11	35.53402357814918	37.0	35.0	37.0	32.0	39.0
12	36.75197943195617	38.0	35.0	39.0	33.0	39.0
13	37.059994973063496	39.0	37.0	39.0	33.0	39.0
14	38.280785897928965	40.0	38.0	41.0	33.0	41.0
15	38.52754973184625	40.0	38.0	41.0	34.0	41.0
16	38.58656354380854	40.0	38.0	41.0	34.0	41.0
17	38.54009102994661	40.0	38.0	41.0	34.0	41.0
18	38.42849849040491	40.0	38.0	41.0	34.0	41.0
19	38.33210668007183	40.0	37.0	41.0	34.0	41.0
20	38.221257884871044	40.0	37.0	41.0	34.0	41.0
21	38.179569439859975	40.0	36.0	41.0	34.0	41.0
22	38.13468798168499	40.0	36.0	41.0	33.0	41.0
23	37.89568925053222	39.0	36.0	41.0	33.0	41.0
24	37.47415034174083	39.0	35.0	41.0	33.0	41.0
25	37.34639287022933	39.0	35.0	41.0	33.0	41.0
26	37.26398260558838	39.0	35.0	41.0	33.0	41.0
27	37.217219377326096	39.0	35.0	41.0	33.0	41.0
28	37.064063763358476	39.0	35.0	41.0	33.0	41.0
29	36.92631358998973	39.0	35.0	41.0	33.0	41.0
30	36.7089833777985	39.0	35.0	41.0	32.0	41.0
31	36.48622467983562	39.0	35.0	41.0	31.0	41.0
32	36.09228425811805	39.0	35.0	41.0	30.0	41.0
33	35.64180594208121	39.0	35.0	41.0	26.0	41.0
34	35.143498444980786	38.0	35.0	41.0	23.0	41.0
35	34.74901414507254	38.0	35.0	41.0	20.0	41.0
36	34.54007588857159	38.0	34.0	40.0	18.0	41.0
37	34.43745552221088	38.0	34.0	40.0	18.0	41.0
38	34.29925958676159	38.0	34.0	40.0	18.0	41.0
39	34.16043982666154	38.0	34.0	40.0	17.0	41.0
40	34.052625363014464	38.0	34.0	40.0	16.0	41.0
41	33.940349038976926	38.0	33.0	40.0	15.0	41.0
42	33.8493832917955	38.0	33.0	40.0	15.0	41.0
43	33.73653038419725	38.0	33.0	40.0	15.0	41.0
44	33.52654495020002	38.0	33.0	40.0	14.0	41.0
45	33.44157276490593	37.0	33.0	40.0	13.0	41.0
46	33.406503523397966	37.0	33.0	40.0	13.0	41.0
47	33.3535523179931	37.0	33.0	40.0	13.0	41.0
48	33.23906474754786	37.0	33.0	40.0	12.0	41.0
49	33.138874874705124	37.0	33.0	40.0	12.0	41.0
50	33.03413774411682	36.0	33.0	40.0	12.0	41.0
51	32.71266091496301	36.0	32.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	3.0
14	9.0
15	26.0
16	107.0
17	256.0
18	679.0
19	1454.0
20	2961.0
21	5097.0
22	8681.0
23	14130.0
24	22890.0
25	35830.0
26	45812.0
27	46138.0
28	40809.0
29	37428.0
30	39188.0
31	45510.0
32	56828.0
33	76155.0
34	111205.0
35	175633.0
36	171501.0
37	262446.0
38	312733.0
39	137569.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.147025779705103	20.678575862831256	24.625629502666396	25.548768854797245
2	42.03203309298924	21.527219649870844	25.965096102307246	10.475651154832672
3	25.918400101750038	21.989092153436637	41.088362036333244	11.00414570848008
4	22.817083104950928	23.707941045542228	40.59148267372456	12.883493175782279
5	19.752165973696403	28.125830882954144	39.472111101353335	12.649892041996116
6	18.093095230164042	35.75139073529545	36.68300925743669	9.472504777103818
7	75.46400743744341	3.178538009393709	19.559385986960248	1.7980685662026339
8	28.6295541470712	63.24231348097184	7.147879753256153	0.9802526187008096
9	71.62136871973618	4.648826089194812	20.70219640786019	3.0276087832088208
10	36.091889976712565	27.12904388273308	25.68449614046351	11.094570000090847
11	27.351803792005956	21.065407711805126	36.194124540837805	15.388663955351115
12	25.976603547321343	19.05009069683636	38.53455716020483	16.438748595637467
13	21.69886227708111	22.842338918481868	39.23045475605731	16.22834404837972
14	16.921334500228635	27.311891127457066	39.556478842956686	16.210295529357612
15	15.567695573570429	23.766386753113824	44.43339460543091	16.23252306788484
16	19.57543584447991	21.02894728076046	43.06945954376008	16.326157330999543
17	18.670405576871246	21.525220988368396	38.544489902217	21.25988353254336
18	19.602508623013073	23.77819702562829	39.25898110659225	17.360313244766385
19	21.713397997098912	26.500616253963255	35.01527764739372	16.77070810154412
20	21.794071243197738	26.30650382622547	34.40150686964184	17.49791806093495
21	20.73611308790174	25.6023693223629	37.84816834786401	15.813349241871352
22	19.29247382813328	22.195317681189263	37.73030788471963	20.78190060595783
23	17.76168081375806	26.484445265443448	36.64370224788854	19.11017167290996
24	18.706926573415984	26.286698907701204	38.61317117930113	16.393203339581675
25	20.09490613861626	25.00876685613574	36.68973202794492	18.206594977303077
26	16.935809654746368	27.375060944034452	35.39411485035779	20.295014550861392
27	17.22186051159678	25.338909397040165	38.16813588475597	19.27109420660709
28	16.67283425342422	25.48796109272275	39.769911665218146	18.06929298863488
29	17.645152791615313	26.02051353487513	38.75901290347979	17.57532077002977
30	19.66137828908519	24.661544844210393	38.66180527586071	17.01527159084371
31	22.9354280920959	23.899751984277195	34.834671326172476	18.330148597454432
32	22.09011540756039	25.760021319056026	35.076751629969024	17.073111643414563
33	21.470348645301176	25.30038973899298	33.94647826758443	19.28278334812141
34	18.703595470911903	26.932327138492102	35.57078441407421	18.793292976521784
35	18.69977984440723	27.127893138231666	35.04580265943111	19.12652435792999
36	22.51304429457848	27.397288482561677	32.548323698371696	17.541343524488145
37	19.931863812416534	29.62149590728633	32.24210452999658	18.204535750300558
38	20.36630014444872	30.177547763467494	30.932072763391787	18.524079328691997
39	20.035006859042884	28.065083686379726	30.611741833499384	21.288167621078006
40	21.02876558426024	26.613025822100955	32.585874308417694	19.77233428522111
41	19.5329794289279	25.962431220303976	32.67345202152498	21.831137329243145
42	19.696264017127923	29.00045726952556	30.477407554334825	20.82587115901169
43	19.778633097228827	27.247994524878795	31.14732255065547	21.82604982723691
44	20.184664209726215	28.580374961010957	31.870232359541035	19.364728469721793
45	19.41009202927736	29.800769787505942	30.483282407842022	20.305855775374674
46	21.41462838523292	28.449008391350034	31.069435317560057	19.066927905856986
47	20.26897138582949	28.61071827654813	31.27178465330794	19.848525684314446
48	19.61880074253303	28.51187538042705	31.83637624499956	20.03294763204036
49	20.53939634366076	26.718228095729827	32.153678899888256	20.588696660721155
50	19.262615036596703	27.040012597624013	33.3903658459032	20.307006519876083
51	18.805890600537218	28.679157291631967	32.53851208735968	19.976440020471138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1635.0
1	2803.0
2	3971.0
3	49618.5
4	95266.0
5	60674.5
6	26083.0
7	26047.0
8	26011.0
9	27319.0
10	28627.0
11	29108.0
12	29589.0
13	28992.0
14	28395.0
15	26636.5
16	24878.0
17	23159.0
18	21440.0
19	19642.0
20	17844.0
21	16897.5
22	15951.0
23	15572.5
24	15194.0
25	15877.5
26	17652.5
27	18744.0
28	20605.0
29	22466.0
30	24790.0
31	27114.0
32	29995.5
33	32877.0
34	36485.5
35	40094.0
36	44670.5
37	49247.0
38	56529.0
39	63811.0
40	72872.5
41	81934.0
42	92621.5
43	103309.0
44	110136.5
45	116964.0
46	120514.5
47	124065.0
48	117138.0
49	110211.0
50	100793.0
51	91375.0
52	84746.5
53	78118.0
54	74611.5
55	71105.0
56	68159.0
57	65213.0
58	60271.5
59	55330.0
60	49238.0
61	43146.0
62	38040.0
63	32934.0
64	27810.0
65	22686.0
66	19168.0
67	15650.0
68	13887.0
69	12124.0
70	10764.0
71	9404.0
72	7649.0
73	5894.0
74	4367.5
75	2213.0
76	1585.0
77	1172.5
78	760.0
79	574.0
80	388.0
81	268.5
82	149.0
83	109.5
84	70.0
85	45.0
86	20.0
87	15.0
88	10.0
89	9.5
90	9.0
91	8.5
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1651105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.07292600106041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60444489805717	28.368681028498553
2	9.449884282299921	7.384692585617773
3	4.302686788786984	5.043556875120421
4	2.707119749475274	4.231003585890263
5	1.8828263476797953	3.6783767277869743
6	1.4693534510134887	3.4447163196511665
7	1.1539973136823112	3.156303615005201
8	0.926736164251693	2.8968234854650374
9	0.7900297851764487	2.7781897801349733
>10	4.623362869068768	29.611577576337517
>50	0.05737981078911919	1.483830136647548
>100	0.027375812264933505	2.268654908945359
>500	0.003682091048237171	0.9715608450679826
>1k	8.004545757037327E-4	0.34766515431933087
>5k	0.0	0.0
>10k+	3.201818302814931E-4	4.3343673755118886
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44257	2.6804473367835477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25035	1.5162572943574153	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2113100014838547E-4	0.0	0.0	0.011628576014245006	0.0
2	1.2113100014838547E-4	0.0	0.0	0.043364898053122	0.0
3	1.2113100014838547E-4	0.0	0.0	0.1009626886236793	0.0
4	1.2113100014838547E-4	0.0	0.0	0.13481880316515304	0.0
5	1.2113100014838547E-4	0.0	0.0	0.27145457133253187	0.0
6	1.2113100014838547E-4	0.0	0.0	0.5429697081651379	0.0
7	1.2113100014838547E-4	0.0	0.0	0.7303593653946903	0.0
8	1.2113100014838547E-4	0.0	0.0	1.047359192783015	0.0
9	1.2113100014838547E-4	0.0	0.0	1.7009215040836287	0.0
10	1.2113100014838547E-4	0.0	0.0	2.4701033550258766	0.0
11	1.2113100014838547E-4	0.0	0.0	2.9348224370951574	0.0
12	1.2113100014838547E-4	0.0	0.0	3.2986999615409074	0.0
13	1.2113100014838547E-4	0.0	0.0	3.526789634820317	0.0
14	1.2113100014838547E-4	0.0	0.0	3.6763258545034994	0.0
15	1.2113100014838547E-4	0.0	0.0	3.7827394381338557	0.0
16	1.2113100014838547E-4	0.0	0.0	3.905324010284022	0.0
17	1.2113100014838547E-4	0.0	0.0	4.033844001441459	0.0
18	1.2113100014838547E-4	0.0	0.0	4.262660460721759	0.0
19	1.2113100014838547E-4	0.0	0.0	4.37319249835716	0.0
20	1.2113100014838547E-4	0.0	0.0	4.495837636007401	0.0
21	1.2113100014838547E-4	0.0	0.0	4.612789616650668	0.0
22	1.2113100014838547E-4	0.0	0.0	4.739492642805878	0.0
23	1.2113100014838547E-4	0.0	0.0	4.88382022948268	0.0
24	1.2113100014838547E-4	0.0	0.0	5.017306591646201	0.0
25	1.2113100014838547E-4	0.0	0.0	5.1259005332792285	0.0
26	1.2113100014838547E-4	0.0	0.0	5.236250874414408	0.0
27	1.2113100014838547E-4	0.0	0.0	5.363317293570064	0.0
28	1.2113100014838547E-4	0.0	0.0	5.483660942217485	0.0
29	1.2113100014838547E-4	0.0	0.0	5.626110998391986	0.0
30	1.2113100014838547E-4	0.0	0.0	5.807807498614564	0.0
31	1.2113100014838547E-4	0.0	0.0	5.942262908779272	0.0
32	1.2113100014838547E-4	0.0	0.0	6.082411475950954	0.0
33	1.2113100014838547E-4	0.0	0.0	6.228010938129313	0.0
34	1.2113100014838547E-4	0.0	0.0	6.376880937311679	0.0
35	1.2113100014838547E-4	0.0	0.0	6.549977136523722	0.0
36	1.2113100014838547E-4	0.0	0.0	6.703328982711578	0.0
37	1.2113100014838547E-4	0.0	0.0	6.869520714915163	0.0
38	1.2113100014838547E-4	0.0	0.0	7.062482398151541	0.0
39	1.8169650022257822E-4	0.0	0.0	7.335451106985928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.034181E-4	45.000004	6
CGTTTTT	26585	0.0	44.28061	1
GTTTTTA	17640	0.0	43.469387	2
TCACGAC	480	0.0	42.656254	25
CACGACG	490	0.0	41.785713	26
TAGCCGT	210	0.0	41.785713	44
GGTACGA	65	0.0	41.53846	2
CGTTAGA	130	0.0	41.53846	2
CGCGTCA	60	3.6379788E-12	41.250004	39
CGGTCTA	505	0.0	40.9901	31
CGACGGT	515	0.0	40.631065	28
TTATTTT	19175	0.0	40.611473	6
CGAGAAT	205	0.0	40.609756	6
AGACTTA	1560	0.0	40.528847	8
CCGCTCG	295	0.0	40.42373	19
TATTTTT	19325	0.0	40.27296	7
CGCATCG	185	0.0	40.135136	21
TTTTATT	19600	0.0	39.845665	4
TTTATTT	19610	0.0	39.74503	5
AGCGATA	205	0.0	39.512196	8
>>END_MODULE