FastQCFastQC Report
Thu 26 May 2016
SRR1546906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546906_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1731205
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209781.2117571287051505No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115690.6682628573739101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGA207.0342777E-445.02
CGTTTTT192800.043.8096471
GTTTTTA133650.042.441082
CGGTCTA2600.042.40384331
AGCGATA2200.040.9090928
CGACGGT2950.039.6610228
CTCGTAG403.4595723E-739.3751
TTATTTT152750.039.137486
TATTTTT152750.039.078567
CACGACG2800.038.57142626
TTTTATT156700.038.2514344
GCGATAT1000.038.259
ACGACGG2950.038.13559327
TTTATTT157000.038.092365
TTTTTAT158350.037.6113053
CGATACG301.1401605E-437.5000041
TCACGAC2900.037.24138325
ACGGGTA552.750312E-936.818185
GAGCGAT13200.036.818187
AGACTTA9700.036.4175228
Produced by FastQC (version 0.11.5)