##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546906_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1731205
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676439820818448	31.0	31.0	34.0	30.0	34.0
2	32.017884652597466	31.0	31.0	34.0	30.0	34.0
3	31.92876869001649	31.0	31.0	34.0	30.0	34.0
4	35.75989902986648	37.0	35.0	37.0	35.0	37.0
5	35.792843712905174	37.0	35.0	37.0	35.0	37.0
6	35.84072481306373	37.0	35.0	37.0	35.0	37.0
7	36.06442102466201	37.0	35.0	37.0	35.0	37.0
8	13.241578553666377	0.0	0.0	35.0	0.0	37.0
9	24.861596402505768	18.0	17.0	38.0	17.0	39.0
10	32.61072143391453	32.0	32.0	37.0	27.0	39.0
11	35.53970962422128	37.0	35.0	37.0	32.0	39.0
12	36.713867508469534	38.0	35.0	39.0	33.0	39.0
13	37.024522803480814	39.0	37.0	39.0	33.0	39.0
14	38.25767312363354	40.0	38.0	41.0	34.0	41.0
15	38.47588587140171	40.0	38.0	41.0	34.0	41.0
16	38.523115402277604	40.0	38.0	41.0	34.0	41.0
17	38.482381347096386	40.0	38.0	41.0	34.0	41.0
18	38.39540608997779	40.0	38.0	41.0	34.0	41.0
19	38.32351570149116	40.0	37.0	41.0	34.0	41.0
20	38.24879491452485	40.0	37.0	41.0	34.0	41.0
21	38.17861200724351	40.0	37.0	41.0	34.0	41.0
22	38.127602450316395	40.0	37.0	41.0	33.0	41.0
23	37.92657137658452	39.0	36.0	41.0	33.0	41.0
24	37.64946958910123	39.0	35.0	41.0	33.0	41.0
25	37.5229236283398	39.0	35.0	41.0	33.0	41.0
26	37.4514751285954	39.0	35.0	41.0	33.0	41.0
27	37.39122229891896	39.0	35.0	41.0	33.0	41.0
28	37.241637472165344	39.0	35.0	41.0	33.0	41.0
29	37.15060723600036	39.0	35.0	41.0	33.0	41.0
30	36.988610823097204	39.0	35.0	41.0	32.0	41.0
31	36.810880282808796	39.0	35.0	41.0	31.0	41.0
32	36.48428522329822	39.0	35.0	41.0	30.0	41.0
33	36.12059461473367	39.0	35.0	41.0	29.0	41.0
34	35.73192314024047	39.0	35.0	41.0	25.0	41.0
35	35.42187089339506	39.0	35.0	41.0	23.0	41.0
36	35.24496001340107	39.0	35.0	41.0	23.0	41.0
37	35.13681453091922	39.0	35.0	41.0	22.0	41.0
38	35.0237505090385	39.0	35.0	41.0	22.0	41.0
39	34.89897094798132	39.0	35.0	40.0	21.0	41.0
40	34.793667994258335	38.0	34.0	40.0	21.0	41.0
41	34.70397266643754	38.0	34.0	40.0	20.0	41.0
42	34.63621465973123	38.0	34.0	40.0	20.0	41.0
43	34.50895532302645	38.0	34.0	40.0	18.0	41.0
44	34.313635877900076	38.0	34.0	40.0	18.0	41.0
45	34.20827689383984	38.0	34.0	40.0	18.0	41.0
46	34.164038920867256	38.0	34.0	40.0	19.0	41.0
47	34.10103078491571	38.0	33.0	40.0	19.0	41.0
48	34.00264266796827	37.0	33.0	40.0	18.0	41.0
49	33.890736221302504	37.0	33.0	40.0	18.0	41.0
50	33.77830181867543	37.0	33.0	40.0	18.0	41.0
51	33.430556173301255	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	2.0
12	3.0
13	11.0
14	12.0
15	37.0
16	123.0
17	297.0
18	730.0
19	1523.0
20	2811.0
21	4930.0
22	7954.0
23	12474.0
24	20159.0
25	30726.0
26	39807.0
27	41173.0
28	37975.0
29	36610.0
30	39481.0
31	46531.0
32	58971.0
33	78817.0
34	114150.0
35	165239.0
36	187447.0
37	300946.0
38	348164.0
39	154069.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.713739851721776	20.027264246579694	24.042213371611105	21.216782530087425
2	40.59103341314287	21.876785244959436	25.064622618349645	12.467558723548049
3	28.884043195346592	23.184198289630633	35.588968377517396	12.342790137505379
4	25.57351671234776	24.825829407840203	34.71899630604117	14.881657573770871
5	22.15838101206963	29.01476139452	34.27808953878946	14.548768054620915
6	19.97602825777421	37.524614358207145	32.52491761518711	9.974439768831536
7	77.69640221695293	4.494037390141549	15.416602886428818	2.392957506476703
8	29.01672534448549	63.88157381708116	5.770720394176311	1.3309804442570348
9	72.19728455035654	6.1444485199615295	17.479212456063838	4.179054473618087
10	37.36380151397437	26.03648903509405	23.068729584306887	13.530979866624692
11	30.982119390828935	23.299955811125777	29.92892234021967	15.789002457825617
12	27.268463295796856	21.412830947230397	34.18306901840048	17.135636738572266
13	22.48064209611225	25.290707917317707	34.708021291528155	17.520628695041893
14	18.73781556776927	28.046245245363778	35.56424571324598	17.651693473620973
15	18.124889888834655	22.648328765224225	41.32601280610904	17.900768539832082
16	20.67536773519023	21.13175504922872	39.66341363385619	18.529463581724865
17	20.3245138501795	20.870029834710504	34.90193246900281	23.90352384610719
18	22.950488243737745	21.93154479105594	35.53825225782042	19.579714707385897
19	25.33616758269529	23.57658394008797	31.857463443093103	19.22978503412363
20	26.664202102004097	22.68599039397414	31.355096594568522	19.29471090945324
21	23.874757755436242	24.435118891176955	32.86849333267868	18.82163002070812
22	22.93096427055144	22.20505370536707	32.59261612576211	22.271365898319377
23	21.61286502753862	25.44401154109421	31.940873553392002	21.002249877975167
24	21.214645290419103	23.425764135385467	35.72170828989057	19.637882284304865
25	21.573586028228892	23.581262762064572	33.951900554815865	20.893250654890668
26	20.401223425302028	25.83587732244304	32.11866878850281	21.644230463752127
27	21.00144119269526	24.12435269075586	33.67255755384255	21.201648562706325
28	19.82024081492371	24.71937176706398	36.03524712555705	19.425140292455257
29	20.882853272720446	24.166173272373868	34.8389127804044	20.112060674501286
30	21.827455442885157	23.4873397431269	34.31026366028287	20.374941153705077
31	25.773724082358818	23.25986812653614	30.611972585569013	20.354435205536028
32	25.562599461069023	24.415710444459208	30.661648967048965	19.360041127422807
33	24.58715172379932	24.341311398707838	30.099439407811325	20.972097469681522
34	22.256520747109672	25.684999754506254	31.46721503230409	20.591264466079984
35	22.629671240552103	25.632724027483743	31.028503267954978	20.709101464009173
36	25.197593583659938	26.916800725506224	28.521116794371547	19.36448889646229
37	22.763624180845135	28.66748883003457	28.601234400316542	19.96765258880375
38	22.584904733985866	29.06241606280019	27.753558937272015	20.59912026594193
39	22.46464168021696	27.262744735603235	28.162176056561762	22.110437527618046
40	23.26235194561014	25.36787959831447	29.66575304484449	21.704015411230905
41	20.814403840099814	25.800872802469954	29.5784727978489	23.806250559581333
42	22.68766552776823	27.022276391299705	27.897909259735272	22.3921488211968
43	22.30007422575605	26.232421925768467	28.630924702735953	22.836579145739528
44	22.306774760932413	27.038565623366384	29.294219921961872	21.360439693739334
45	21.79799619340286	27.53382759407465	28.67817502837619	21.9900011841463
46	22.787076053962412	27.08789542544066	28.724327852565125	21.400700668031806
47	22.105354363001492	26.87347829979696	29.51678166363891	21.504385673562634
48	22.16768089278855	25.616608085119903	30.444228153222756	21.771482868868794
49	22.324277020918956	25.339229034112076	30.38005319993877	21.956440745030196
50	20.57930747658423	27.202093339610272	30.807847713009146	21.410751470796352
51	21.01807700416762	27.09673320028535	29.55294144829757	22.332248347249458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1351.0
1	2679.0
2	4007.0
3	29741.0
4	55475.0
5	38270.0
6	21065.0
7	20519.0
8	19973.0
9	19779.5
10	19586.0
11	19082.0
12	18578.0
13	17791.5
14	17005.0
15	15984.0
16	14963.0
17	14318.5
18	13674.0
19	12967.0
20	12260.0
21	11824.5
22	11389.0
23	11772.5
24	12156.0
25	13076.5
26	16005.5
27	18014.0
28	19799.5
29	21585.0
30	24122.5
31	26660.0
32	30002.0
33	33344.0
34	37047.0
35	40750.0
36	45105.5
37	49461.0
38	58294.5
39	67128.0
40	76189.5
41	85251.0
42	92120.0
43	98989.0
44	104237.5
45	109486.0
46	114980.5
47	120475.0
48	121895.0
49	123315.0
50	119190.0
51	115065.0
52	109145.0
53	103225.0
54	98213.0
55	93201.0
56	88920.0
57	84639.0
58	80217.0
59	75795.0
60	69402.5
61	63010.0
62	57091.5
63	51173.0
64	44220.0
65	37267.0
66	31646.0
67	26025.0
68	22370.5
69	18716.0
70	16049.5
71	13383.0
72	11294.0
73	9205.0
74	7243.0
75	3978.0
76	2675.0
77	2065.0
78	1455.0
79	1050.0
80	645.0
81	459.5
82	274.0
83	217.0
84	160.0
85	102.0
86	44.0
87	29.5
88	15.0
89	12.5
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1731205.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.42894353876536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60481592542948	23.220685327494394
2	6.905537324594676	4.478785600082351
3	3.5403021803734154	3.4442477855249223
4	2.429718740651198	3.151728474226311
5	2.017517408245455	3.2712979060234044
6	1.6500945078059759	3.2106492976360093
7	1.4126446120896166	3.2067400656056684
8	1.2591802250139617	3.2667107537686104
9	1.1009377810767669	3.2132024228008884
>10	7.977987765192328	43.908097056863376
>50	0.07161605898083005	1.5399836463547312
>100	0.027053346500705195	1.6610542721113817
>500	0.0020382403215813945	0.4004936715874015
>1k	1.8529457468921767E-4	0.07060466078247514
>5k	0.0	0.0
>10k+	3.7058914937843535E-4	1.9557190591380578
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20978	1.2117571287051505	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11569	0.6682628573739101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007798036627666856	0.0
2	0.0	0.0	0.0	0.025069243677091967	0.0
3	0.0	0.0	0.0	0.05106269910264816	0.0
4	0.0	0.0	0.0	0.07798036627666856	0.0
5	0.0	0.0	0.0	0.15492099433631487	0.0
6	0.0	0.0	0.0	0.31989279143717814	0.0
7	0.0	0.0	0.0	0.4331664938583241	0.0
8	0.0	0.0	0.0	0.6633529824601939	0.0
9	0.0	0.0	0.0	1.0427419052047562	0.0
10	0.0	0.0	0.0	1.5601271946418824	0.0
11	0.0	0.0	0.0	1.8368708500726372	0.0
12	0.0	0.0	0.0	2.0792453811073788	0.0
13	0.0	0.0	0.0	2.209501474406555	0.0
14	0.0	0.0	0.0	2.272059057130727	0.0
15	0.0	0.0	0.0	2.327858341444254	0.0
16	0.0	0.0	0.0	2.411326214977429	0.0
17	0.0	0.0	0.0	2.4962959326018583	0.0
18	0.0	0.0	0.0	2.6490796872698494	0.0
19	0.0	0.0	0.0	2.718222278701829	0.0
20	0.0	0.0	0.0	2.794411984715848	0.0
21	0.0	0.0	0.0	2.8753382759407464	0.0
22	0.0	0.0	0.0	2.966604186101588	0.0
23	0.0	0.0	0.0	3.0727152474721366	0.0
24	0.0	0.0	0.0	3.1568185165823803	0.0
25	0.0	0.0	0.0	3.2280983476826837	0.0
26	0.0	0.0	0.0	3.301977524325542	0.0
27	0.0	0.0	0.0	3.3829038155504403	0.0
28	0.0	0.0	0.0	3.468335639049102	0.0
29	0.0	0.0	0.0	3.5593127330385483	0.0
30	0.0	0.0	0.0	3.683850266144102	0.0
31	0.0	0.0	0.0	3.784935926132376	0.0
32	0.0	0.0	0.0	3.907278456335327	0.0
33	0.0	0.0	0.0	4.020667685225031	0.0
34	0.0	0.0	0.0	4.122099924618979	0.0
35	0.0	0.0	0.0	4.23392954618315	0.0
36	0.0	0.0	0.0	4.338885342868118	0.0
37	0.0	0.0	0.0	4.45296773056917	0.0
38	0.0	0.0	0.0	4.57773631661184	0.0
39	0.0	0.0	0.0	4.732021915371085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCGA	20	7.0342777E-4	45.0	2
CGTTTTT	19280	0.0	43.809647	1
GTTTTTA	13365	0.0	42.44108	2
CGGTCTA	260	0.0	42.403843	31
AGCGATA	220	0.0	40.909092	8
CGACGGT	295	0.0	39.66102	28
CTCGTAG	40	3.4595723E-7	39.375	1
TTATTTT	15275	0.0	39.13748	6
TATTTTT	15275	0.0	39.07856	7
CACGACG	280	0.0	38.571426	26
TTTTATT	15670	0.0	38.251434	4
GCGATAT	100	0.0	38.25	9
ACGACGG	295	0.0	38.135593	27
TTTATTT	15700	0.0	38.09236	5
TTTTTAT	15835	0.0	37.611305	3
CGATACG	30	1.1401605E-4	37.500004	1
TCACGAC	290	0.0	37.241383	25
ACGGGTA	55	2.750312E-9	36.81818	5
GAGCGAT	1320	0.0	36.81818	7
AGACTTA	970	0.0	36.417522	8
>>END_MODULE