FastQCFastQC Report
Thu 26 May 2016
SRR1546905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546905_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences728385
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103551.4216382819525388No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57690.7920261949381165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG302.1644028E-645.0000041
GTTACGA253.8893937E-545.02
ATAACGC551.8189894E-1245.011
CGTTTTT88400.044.160071
CGGTCTA1450.043.4482831
GTTTTTA59150.042.8317832
AACACGT1600.042.187541
TCGTTTA501.0804797E-940.538
CGCATCG501.0804797E-940.521
TATGCGA501.0804797E-940.52
TTATTTT65950.040.0189556
AAACACG1800.040.040
TCGTAGA451.9266736E-840.02
ACGACCA1800.040.028
ACACGAC1750.039.85714326
TATTTTT66350.039.7776957
CACGTGA1700.039.70588343
GTTAGCG403.455898E-739.3751
CGTTAGA403.455898E-739.3752
TCACGAC1550.039.19354625
Produced by FastQC (version 0.11.5)