FastQCFastQC Report
Thu 26 May 2016
SRR1546904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546904_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1596056
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149470.93649596254768No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83460.5229139829680162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16210.10156285243124301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.1660344E-645.0000041
CGTCTAC253.891489E-545.00000432
CGTTAAT253.891489E-545.00000422
AACGCGT253.891489E-545.00000414
TATCACG207.0341124E-445.010
CGTTTTT111200.043.239661
CGGTCTA8700.042.41379531
TAACGCC3100.042.0967712
TCTCACG8900.041.9662923
ACGACGG8900.041.9662927
CGACGGT8850.041.9491528
TCACGAC8950.041.73184225
CACGACG8900.041.7134826
GTTTTTA77550.041.663442
TTAGCGA1900.041.447372
AGACTTA17450.041.2607468
CGCATCG2800.040.9821421
ACGGTCT9150.040.3278730
ACCGCTC2700.040.00000418
GACGGTC9300.039.91935729
Produced by FastQC (version 0.11.5)