FastQCFastQC Report
Thu 26 May 2016
SRR1546903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546903_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2007476
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366011.8232347485100695No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204491.0186423150264312No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACG253.89185E-545.01
CGTTTTT324300.043.882981
GTTTTTA215300.043.254762
TATGCGA800.042.18752
CGGTCTA3650.041.3013731
TATTTTT236750.040.172127
TTATTTT236950.040.1572076
TTTTATT241250.039.5906754
AGACTTA17450.039.584538
TTTATTT242150.039.3877755
CGCATCG1200.039.37499621
ACGACGG3900.039.2307727
CACGACG4000.038.812526
TCACGAC4150.038.49397325
CTATCTC11450.038.318786
TTTTTAT250200.038.210433
CGACGGT4150.037.95180528
ACGGTCT4050.037.7777830
GAGACTT22150.037.6862267
TCTCACG4200.037.523