FastQCFastQC Report
Thu 26 May 2016
SRR1546902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546902_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1847570
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314801.703859664315831No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174570.9448627115616729No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT258050.044.0147251
GTTTTTA170800.043.3928572
CGTTAGA1150.043.0434762
TATAGCG850.042.352941
TTACGAG3300.040.9090923
CGGTCTA3300.040.90909231
GCGATAT1600.040.781259
GAGCGAT14050.039.71537
CGCATCG1250.039.60000221
TTATTTT192800.039.5500536
TATTTTT193100.039.4653057
AGACTTA15750.039.428578
TTTATTT195600.039.0644155
TTTTATT196200.039.048174
GTTGCGA1050.038.571432
CGTAAGA1000.038.252
CGACGAG1850.037.7027053
TTTTTAT203250.037.5940973
AATTCGC301.14019036E-437.50000413
CACGACG3550.037.39436726