FastQCFastQC Report
Thu 26 May 2016
SRR1546901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546901_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1353465
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140951.0414011444699345No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79000.5836870550771538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC750.045.00000412
CGGTCTA2650.044.15094431
CGTTTTT117500.043.6787221
TCGTAGA900.042.52
GTTTTTA79450.042.422912
CACGACG3050.040.57377226
GTTGCGA501.0822987E-940.52
GAGCGAT10400.040.2403877
TCACGAC3050.039.83606725
CGACGGT3050.039.83606728
ATAACGC850.039.70588311
CGATACG403.4588265E-739.37500410
AGACTTA8600.039.2441868
TTACGAG1500.039.0000043
TATTTTT91050.038.3278437
CACGTGA2350.038.2978743
ACGGTCT3100.037.74193630
CACGACC2450.037.6530627
CGTTAGA900.037.52
TTATTTT93850.037.304216
Produced by FastQC (version 0.11.5)