##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546901_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1353465 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685216093508142 31.0 31.0 34.0 30.0 34.0 2 32.02952422116567 31.0 31.0 34.0 30.0 34.0 3 31.98399367549216 33.0 31.0 34.0 30.0 34.0 4 35.7949226614652 37.0 35.0 37.0 35.0 37.0 5 35.80705300838958 37.0 35.0 37.0 35.0 37.0 6 35.85838422123956 37.0 35.0 37.0 35.0 37.0 7 36.061089869335376 37.0 35.0 37.0 35.0 37.0 8 13.262684295493418 0.0 0.0 35.0 0.0 37.0 9 24.868818920326717 18.0 17.0 38.0 17.0 39.0 10 32.6052642661613 32.0 32.0 37.0 27.0 39.0 11 35.54088210629754 37.0 35.0 37.0 32.0 39.0 12 36.690170045032566 38.0 35.0 39.0 33.0 39.0 13 36.97759749975064 39.0 37.0 39.0 33.0 39.0 14 38.19404343666072 40.0 37.0 41.0 33.0 41.0 15 38.43566327906522 40.0 38.0 41.0 34.0 41.0 16 38.49943145925458 40.0 38.0 41.0 34.0 41.0 17 38.46729985629477 40.0 38.0 41.0 34.0 41.0 18 38.38615036221845 40.0 37.0 41.0 34.0 41.0 19 38.332199207219986 40.0 37.0 41.0 34.0 41.0 20 38.25669596184608 40.0 37.0 41.0 34.0 41.0 21 38.183998847402776 40.0 37.0 41.0 34.0 41.0 22 38.12190562740817 40.0 37.0 41.0 33.0 41.0 23 37.94690147140857 40.0 36.0 41.0 33.0 41.0 24 37.7350917829423 40.0 35.0 41.0 33.0 41.0 25 37.62447717525019 40.0 35.0 41.0 33.0 41.0 26 37.538009479373315 39.0 35.0 41.0 33.0 41.0 27 37.49308478608608 40.0 35.0 41.0 33.0 41.0 28 37.358085358690474 39.0 35.0 41.0 33.0 41.0 29 37.295769007695064 39.0 35.0 41.0 33.0 41.0 30 37.16678968425486 39.0 35.0 41.0 32.0 41.0 31 37.03317115699335 40.0 35.0 41.0 32.0 41.0 32 36.774306686911004 40.0 35.0 41.0 31.0 41.0 33 36.47426715873702 40.0 35.0 41.0 30.0 41.0 34 36.16410694033462 39.0 35.0 41.0 29.0 41.0 35 35.91470854436576 39.0 35.0 41.0 27.0 41.0 36 35.7618785857041 39.0 35.0 41.0 26.0 41.0 37 35.694771567790816 39.0 35.0 41.0 25.0 41.0 38 35.58895797083781 39.0 35.0 41.0 25.0 41.0 39 35.47264835071465 39.0 35.0 41.0 24.0 41.0 40 35.35356215343581 39.0 35.0 41.0 24.0 41.0 41 35.26143417081343 39.0 35.0 40.0 23.0 41.0 42 35.181893879782635 39.0 35.0 40.0 23.0 41.0 43 35.06560199192443 38.0 35.0 40.0 23.0 41.0 44 34.8822518498816 38.0 34.0 40.0 23.0 41.0 45 34.77917049942185 38.0 34.0 40.0 23.0 41.0 46 34.73639805979467 38.0 34.0 40.0 23.0 41.0 47 34.68662285319532 38.0 34.0 40.0 23.0 41.0 48 34.58858854865105 38.0 34.0 40.0 23.0 41.0 49 34.47670238979212 38.0 34.0 40.0 22.0 41.0 50 34.36861167447994 37.0 34.0 40.0 22.0 41.0 51 34.00587307392507 37.0 33.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 1.0 11 1.0 12 3.0 13 3.0 14 16.0 15 29.0 16 88.0 17 221.0 18 503.0 19 975.0 20 2002.0 21 3410.0 22 5297.0 23 8622.0 24 13197.0 25 19927.0 26 25732.0 27 28103.0 28 26568.0 29 26494.0 30 29034.0 31 35378.0 32 44872.0 33 61255.0 34 90288.0 35 133162.0 36 147664.0 37 238981.0 38 287359.0 39 124255.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.04989046632163 21.844303325169104 23.679592748981317 19.42621345952795 2 38.330211715855235 23.12169136253985 25.721610828503138 12.82648609310178 3 29.011389286017742 23.774682019852747 33.93201892919285 13.28190976493666 4 26.165804065860588 25.73882590240605 33.163103589675394 14.932266442057976 5 22.550712430687163 29.909232968713635 32.21413187633223 15.325922724266974 6 20.968920511428074 38.65951465313104 30.440535957708548 9.931028877732338 7 79.9376415348753 4.842238255145127 12.94603111273657 2.274089097243002 8 29.979423184197596 63.8874296712512 4.8372141134052224 1.2959330311459845 9 74.25829260453725 6.550372562275345 14.736103260889644 4.45523157229777 10 39.3851337123605 26.742693752701403 20.492439774948004 13.379732759990098 11 33.33702755520091 23.232444134129807 27.506141643854846 15.924386666814435 12 29.899184685233827 21.32223589084313 32.276564225894276 16.502015198028765 13 23.66902727444005 26.49237327895439 32.61096518934734 17.227634257258224 14 18.701998204608174 30.062617060655427 33.659015933178914 17.576368801557486 15 18.37535510707702 23.485350563184124 40.50950707997621 17.629787249762646 16 20.85624674446698 21.041031722283176 39.088857118580826 19.013864414669015 17 21.161758892915593 20.83186488014097 32.7936814029177 25.21269482402574 18 23.291034492949578 22.92804025224147 33.82466484172107 19.95626041308789 19 26.436294991004573 24.020421658483965 29.796411432877832 19.74687191763363 20 27.272075746325175 23.497910917533886 29.613030259371314 19.616983076769625 21 25.159645798007336 25.059458500958648 31.059244236090333 18.72165146494368 22 23.64368491242847 22.76475564569457 30.855692611186843 22.735866830690117 23 21.939835902664644 25.829038800412274 30.30222429098647 21.928901005936616 24 21.760813910961865 23.938631586335813 34.54060503965747 19.75994946304485 25 22.067655979282804 24.201438529995233 32.87059510220065 20.860310388521313 26 19.95145792466004 26.985625782713257 31.090275699777976 21.972640592848723 27 21.00268569929773 25.135190049243977 32.594415075380596 21.267709176077698 28 19.743103811328698 25.438190126822636 35.32230238683675 19.496403675011916 29 20.41161020048542 25.147972056905793 33.918498077157516 20.521919665451268 30 22.085388244247174 25.04704591548359 32.870816755512706 19.996749084756534 31 25.89568256290336 24.32408669599879 29.20060733007503 20.57962341102282 32 26.152430982699958 24.984318028172137 29.88913640175402 18.97411458737389 33 25.092928151078898 25.315394191944378 28.85061675034079 20.74106090663593 34 21.87732966866524 26.540176509920833 30.52136553217113 21.061128289242795 35 22.098982980719857 26.41043543793153 30.069562197766476 21.42101938358214 36 24.659004850513313 27.541088982722123 27.76377667689966 20.0361294898649 37 22.31346950235137 30.000849671029545 27.49963981336791 20.186041013251174 38 22.198726971144435 30.385713705193705 26.032959847502525 21.38259947615934 39 22.367405141617997 28.034784793105104 26.876794006494443 22.72101605878246 40 23.29406375488099 26.211169110394433 28.38278049303085 22.111986641693726 41 20.804749291632955 25.833397982216017 28.73085007739395 24.63100264875708 42 22.497663404668756 27.63396172047301 26.807933710882807 23.060441163975426 43 22.31243512022845 26.89482180920822 27.42472099389345 23.36802207666988 44 22.05021925206784 27.534217730048432 28.670708145389796 21.74485487249393 45 21.028028061309307 28.918516548266854 27.96548119086936 22.087974199554477 46 22.61595238886857 28.166964051527003 28.254295456476523 20.962788103127895 47 22.749166029413395 27.151200806818053 28.634504771087542 21.465128392681006 48 22.134521395085947 26.228014762110586 29.92349266512248 21.71397117768099 49 22.033669138101097 25.544140409984745 30.436546198091563 21.985644253822596 50 20.607256190592295 27.400856320628904 30.188885564089208 21.803001924689593 51 21.357404883022465 27.808328992622638 28.53749450484497 22.296771619509926 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 917.0 1 2026.0 2 3135.0 3 19983.5 4 36832.0 5 25042.5 6 13253.0 7 12934.0 8 12615.0 9 12604.0 10 12593.0 11 12311.5 12 12030.0 13 11479.0 14 10928.0 15 10222.5 16 9517.0 17 9067.5 18 8618.0 19 8363.5 20 8109.0 21 8252.0 22 8395.0 23 8866.5 24 9338.0 25 10369.0 26 12714.5 27 14029.0 28 16069.0 29 18109.0 30 20253.0 31 22397.0 32 25124.0 33 27851.0 34 30782.5 35 33714.0 36 36920.0 37 40126.0 38 47869.0 39 55612.0 40 64527.0 41 73442.0 42 80298.0 43 87154.0 44 89816.0 45 92478.0 46 93958.0 47 95438.0 48 92906.5 49 90375.0 50 87695.0 51 85015.0 52 81037.0 53 77059.0 54 74433.0 55 71807.0 56 70724.5 57 69642.0 58 66276.0 59 62910.0 60 57431.0 61 51952.0 62 46225.0 63 40498.0 64 34502.5 65 28507.0 66 24125.0 67 19743.0 68 16919.0 69 14095.0 70 12098.0 71 10101.0 72 8362.5 73 6624.0 74 5096.5 75 2649.5 76 1730.0 77 1311.5 78 893.0 79 703.5 80 514.0 81 380.0 82 246.0 83 157.5 84 69.0 85 61.5 86 54.0 87 36.0 88 18.0 89 11.5 90 5.0 91 4.0 92 3.0 93 2.5 94 2.0 95 1.0 96 0.0 97 2.0 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1353465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.04504516021511 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48787190428015 21.882991665158354 2 6.795823616067991 4.219533021233743 3 3.6623106778690606 3.4108980115554903 4 2.619890447242924 3.25338468997891 5 2.076107761196994 3.2226429601916884 6 1.7116882035923013 3.188366254843829 7 1.4775986062534365 3.211048082186619 8 1.3163838456667996 3.2693756749522747 9 1.139720805750963 3.1844415496117864 >10 8.601278430054132 45.802738596055924 >50 0.0803212131550993 1.5892746985237465 >100 0.028360695997381516 1.6756327996596883 >500 0.0019227584529360226 0.35218352572114653 >1k 2.4034480661700282E-4 0.09632816349703394 >5k 2.4034480661700282E-4 0.5894597146603936 >10k+ 2.4034480661700282E-4 1.0517005921693985 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14095 1.0414011444699345 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7900 0.5836870550771538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0052457950519592305 0.0 2 0.0 0.0 0.0 0.018914415961993846 0.0 3 0.0 0.0 0.0 0.037237756425175385 0.0 4 0.0 0.0 0.0 0.05859035881977 0.0 5 0.0 0.0 0.0 0.11880617526127384 0.0 6 0.0 0.0 0.0 0.21374767725800076 0.0 7 0.0 0.0 0.0 0.2750717602597777 0.0 8 0.0 0.0 0.0 0.4266826257051346 0.0 9 0.0 0.0 0.0 0.6642949762276823 0.0 10 0.0 0.0 0.0 1.0154677069595446 0.0 11 0.0 0.0 0.0 1.1985533427166568 0.0 12 0.0 0.0 0.0 1.3630201002611815 0.0 13 0.0 0.0 0.0 1.443775790286413 0.0 14 0.0 0.0 0.0 1.4838950397682984 0.0 15 0.0 0.0 0.0 1.5249747869357537 0.0 16 0.0 0.0 0.0 1.5873332520604522 0.0 17 0.0 0.0 0.0 1.6562674321094377 0.0 18 0.0 0.0 0.0 1.7707883100043222 0.0 19 0.0 0.0 0.0 1.82206410952629 0.0 20 0.0 0.0 0.0 1.8821321570930907 0.0 21 0.0 0.0 0.0 1.9489236884588814 0.0 22 0.0 0.0 0.0 2.0169712552596484 0.0 23 0.0 0.0 0.0 2.099352402906614 0.0 24 0.0 0.0 0.0 2.16880377401706 0.0 25 0.0 0.0 0.0 2.2234782576571983 0.0 26 0.0 0.0 0.0 2.2794826611696646 0.0 27 0.0 0.0 0.0 2.3467174991595647 0.0 28 0.0 0.0 0.0 2.411440266279512 0.0 29 0.0 0.0 0.0 2.4834037082599107 0.0 30 0.0 0.0 0.0 2.5827043920603785 0.0 31 0.0 0.0 0.0 2.6649377708326405 0.0 32 0.0 0.0 0.0 2.7609875393896406 0.0 33 0.0 0.0 0.0 2.84787563771505 0.0 34 0.0 0.0 0.0 2.937719113534521 0.0 35 0.0 0.0 0.0 3.03997517482905 0.0 36 0.0 0.0 0.0 3.1290798062750054 0.0 37 0.0 0.0 0.0 3.224021308271732 0.0 38 0.0 0.0 0.0 3.334035235488173 0.0 39 0.0 0.0 0.0 3.4702781379644096 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCC 75 0.0 45.000004 12 CGGTCTA 265 0.0 44.150944 31 CGTTTTT 11750 0.0 43.678722 1 TCGTAGA 90 0.0 42.5 2 GTTTTTA 7945 0.0 42.42291 2 CACGACG 305 0.0 40.573772 26 GTTGCGA 50 1.0822987E-9 40.5 2 GAGCGAT 1040 0.0 40.240387 7 TCACGAC 305 0.0 39.836067 25 CGACGGT 305 0.0 39.836067 28 ATAACGC 85 0.0 39.705883 11 CGATACG 40 3.4588265E-7 39.375004 10 AGACTTA 860 0.0 39.244186 8 TTACGAG 150 0.0 39.000004 3 TATTTTT 9105 0.0 38.327843 7 CACGTGA 235 0.0 38.29787 43 ACGGTCT 310 0.0 37.741936 30 CACGACC 245 0.0 37.65306 27 CGTTAGA 90 0.0 37.5 2 TTATTTT 9385 0.0 37.30421 6 >>END_MODULE