FastQCFastQC Report
Thu 26 May 2016
SRR1546900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546900_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2374494
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147060.6193319503018327No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79980.3368296571816985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG551.8189894E-1245.01
TAATCGT253.8920665E-545.013
CGTTTTT175050.043.778921
CGACGGT3650.042.53424528
CGGTCTA3750.041.431
GTTTTTA122650.041.2209552
ACGCGGA556.184564E-1140.9090922
GAGCGAT18900.040.7142837
CCGGTTA501.0822987E-940.537
CGTAAGA1950.040.3846172
GCGCGAC9200.040.1086929
TATGCGA1800.040.02
ATTGCGA2500.039.62
CACGACG3950.039.303826
TGCGCGA1550.039.1935462
AGGGGAA141650.039.1069538
TATAGCG1900.039.0789451
GAGGGGA142650.038.7223977
TCACGAC4100.038.41463525
TGCGAGC5850.038.076924
Produced by FastQC (version 0.11.5)