FastQCFastQC Report
Thu 26 May 2016
SRR1546899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546899_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3080828
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT793532.5757036744667343No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434181.4092964618602533No Hit
CGTTTCTATCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT44520.14450660666548085No Hit
CGTTTTCAGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT38570.1251936167809433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT511150.044.471781
GTTTTTA340050.043.365682
CACGACG8750.043.19999726
CGGTCTA8900.042.97752831
CGACGGT9400.041.1702128
ATAACGC3450.041.08695611
TTATTTT365800.040.571356
TGTACGA2500.040.5000042
TCTCACG9350.040.42780723
ACGACGG9350.040.42780727
TATTTTT368100.040.299517
AGACTTA24350.040.2874768
TTTATTT372250.039.910685
TTTTATT375300.039.6942444
TCACGAC9600.039.6093825
GACGGTC9850.039.28933729
CGTAAGA2350.039.2553182
CTATCTC24350.039.0862436
ACGGTCT9700.038.9690730
GCGCGAC13000.038.769239