FastQCFastQC Report
Thu 26 May 2016
SRR1546898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546898_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1360030
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170441.2532076498312537No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93980.6910141688050999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA207.0337433E-445.014
CGTAAGA800.045.02
ACGCGTA253.891184E-545.015
CGTTTTT148950.043.625381
TATAGCG750.042.0000041
GTTTTTA101150.041.93032
CGGTCTA2450.039.48979631
TATTTTT115150.038.532357
TTATTTT115150.038.473736
TCACGAC2350.038.2978725
TTTTATT117300.037.979544
CGACGGT2550.037.94117728
TTTATTT117150.037.9321375
ACACGAC4050.037.77777526
GAGCGAT11250.037.67
CACGACG2400.037.49999626
ACGACGG2400.037.49999627
TCTCACG2550.037.05882323
TTTTTAT120350.036.960953
CGAGTAC552.750312E-936.818186
Produced by FastQC (version 0.11.5)