##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546898_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1360030
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679031344896803	31.0	31.0	34.0	30.0	34.0
2	32.024570781526876	31.0	31.0	34.0	30.0	34.0
3	31.92823320073822	31.0	31.0	34.0	30.0	34.0
4	35.75919207664537	37.0	35.0	37.0	35.0	37.0
5	35.79036785953251	37.0	35.0	37.0	35.0	37.0
6	35.84293287648067	37.0	35.0	37.0	35.0	37.0
7	36.059253104710926	37.0	35.0	37.0	35.0	37.0
8	13.140004999889708	0.0	0.0	35.0	0.0	37.0
9	24.807922619353985	18.0	17.0	38.0	17.0	39.0
10	32.58607604244024	32.0	32.0	37.0	27.0	39.0
11	35.53923222281861	37.0	35.0	37.0	32.0	39.0
12	36.7090299478688	38.0	35.0	39.0	33.0	39.0
13	37.01269163180224	39.0	37.0	39.0	33.0	39.0
14	38.242334360271464	40.0	37.0	41.0	33.0	41.0
15	38.452442225539144	40.0	38.0	41.0	34.0	41.0
16	38.49991103137431	40.0	38.0	41.0	34.0	41.0
17	38.44338139599862	40.0	38.0	41.0	34.0	41.0
18	38.3421909810813	40.0	37.0	41.0	34.0	41.0
19	38.278847525422236	40.0	37.0	41.0	34.0	41.0
20	38.203908737307266	40.0	37.0	41.0	34.0	41.0
21	38.14212333551466	40.0	37.0	41.0	33.0	41.0
22	38.102115394513355	40.0	37.0	41.0	33.0	41.0
23	37.897086828967005	39.0	36.0	41.0	33.0	41.0
24	37.61052256200231	39.0	35.0	41.0	33.0	41.0
25	37.5086034866878	39.0	35.0	41.0	33.0	41.0
26	37.43107652037087	39.0	35.0	41.0	33.0	41.0
27	37.375938030778734	39.0	35.0	41.0	33.0	41.0
28	37.207258663411835	39.0	35.0	41.0	33.0	41.0
29	37.12696190525209	39.0	35.0	41.0	33.0	41.0
30	36.94179172518253	39.0	35.0	41.0	32.0	41.0
31	36.772084439313836	39.0	35.0	41.0	31.0	41.0
32	36.433053682639354	39.0	35.0	41.0	30.0	41.0
33	36.08096806688088	39.0	35.0	41.0	29.0	41.0
34	35.66763380219554	39.0	35.0	41.0	25.0	41.0
35	35.37144621809813	39.0	35.0	41.0	23.0	41.0
36	35.18201216149644	39.0	35.0	41.0	22.0	41.0
37	35.13133533819107	39.0	35.0	41.0	22.0	41.0
38	35.008431431659595	39.0	35.0	41.0	21.0	41.0
39	34.899511040197645	39.0	35.0	40.0	21.0	41.0
40	34.78657529613317	39.0	34.0	40.0	21.0	41.0
41	34.69059432512518	38.0	34.0	40.0	20.0	41.0
42	34.60762997875047	38.0	34.0	40.0	19.0	41.0
43	34.479797504466816	38.0	34.0	40.0	18.0	41.0
44	34.2737263148607	38.0	34.0	40.0	18.0	41.0
45	34.174110865201506	38.0	34.0	40.0	18.0	41.0
46	34.130174334389686	38.0	34.0	40.0	18.0	41.0
47	34.078982816555516	38.0	33.0	40.0	18.0	41.0
48	33.98833775725535	37.0	33.0	40.0	18.0	41.0
49	33.877812989419354	37.0	33.0	40.0	18.0	41.0
50	33.77076093909693	37.0	33.0	40.0	18.0	41.0
51	33.437485937810195	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	7.0
14	14.0
15	36.0
16	88.0
17	210.0
18	524.0
19	1190.0
20	2282.0
21	3892.0
22	6293.0
23	10104.0
24	16078.0
25	24342.0
26	31157.0
27	32711.0
28	30403.0
29	29392.0
30	30934.0
31	36935.0
32	46312.0
33	62071.0
34	90339.0
35	130793.0
36	146508.0
37	235695.0
38	273084.0
39	118601.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.5089446556326	20.21727461894223	24.09902722734057	21.174753498084602
2	41.028727307485866	21.827533216179056	24.914156305375617	12.229583170959463
3	28.925906046190157	22.780673955721564	35.56340668955832	12.730013308529958
4	25.21091446512209	24.797247119548835	34.92592075174812	15.065917663580949
5	22.14524679602656	28.369815371719742	34.29666992639868	15.18826790585502
6	20.384991507540278	36.84058439887355	32.80773218237833	9.96669191120784
7	77.43248310699029	4.542105688845099	15.66075748329081	2.3646537208738043
8	28.63245663698595	64.24483283456982	5.77457850194481	1.3481320264994154
9	71.54474533649993	6.220892186201775	17.579538686646618	4.654823790651677
10	38.08063057432557	25.48002617589318	23.246766615442308	13.192576634338948
11	31.426292067086752	23.37448438637383	29.685962809643907	15.513260736895509
12	28.267979382807734	20.700425725903106	34.61203061697169	16.419564274317477
13	22.589354646588678	25.02856554634824	34.377991661948634	18.004088145114448
14	18.702822731851505	27.60909685815754	35.85266501474232	17.835415395248635
15	18.55422306860878	22.18848113644552	41.69165386057661	17.565641934369093
16	21.480334992610455	20.45888693631758	39.86691470041102	18.193863370660942
17	20.93042065248561	20.559105313853372	34.65033859547216	23.860135438188866
18	23.222575972588842	21.97804460195731	35.58649441556436	19.212885009889487
19	25.032756630368446	24.280126173687343	31.776210818878997	18.910906377065213
20	26.985728255994353	22.82559943530657	31.462173628522898	18.726498680176174
21	24.301155121578198	24.35255104666809	32.84295199370602	18.50334183804769
22	23.053756167143373	21.951354014249684	33.467276457136975	21.527613361469967
23	22.244362256714926	24.46835731564745	32.53972338845467	20.747557039182958
24	22.07686595148636	22.99243399042668	35.38304302110983	19.547657036977125
25	21.750917259178106	23.49462879495305	33.808298346360004	20.946155599508835
26	19.97632405167533	25.033712491636212	33.316765071358724	21.673198385329737
27	20.491974441740254	23.968662455975238	34.386962052307666	21.152401049976838
28	19.2961919957648	25.180694543502717	36.34669823459777	19.17641522613472
29	21.123284045204883	24.158364153731902	35.16194495709654	19.556406843966677
30	22.016352580457784	23.44095350837849	35.365984573869696	19.176709337294028
31	25.696124350198158	22.794717763578745	31.506143246840146	20.003014639382954
32	25.560097939016053	24.513062211862973	31.288721572318256	18.638118276802718
33	24.967463953001037	24.273361617023152	30.538296949332	20.22087748064381
34	22.003558745027682	25.417527554539237	32.91978853407645	19.659125166356624
35	21.81216590810497	26.101630111100487	31.86723822268626	20.218965758108276
36	24.459313397498583	26.96411108578487	30.006985140033677	18.569590376682868
37	23.15640096174349	27.531598567678657	29.31898561061153	19.993014859966323
38	22.008705690315654	29.134283802563175	28.247465129445676	20.60954537767549
39	22.80265876488019	26.231333132357378	28.74149834930112	22.224509753461323
40	23.204782247450424	25.200326463386837	29.64978713704845	21.945104152114293
41	20.667926442799057	25.68193348676132	29.70574178510768	23.944398285331943
42	22.3320073821901	26.286626030308152	28.038278567384545	23.343088020117204
43	22.201789666404416	26.12258553120152	28.56503165371352	23.110593148680543
44	22.174290273008683	26.871466070601386	29.54912759277369	21.405116063616244
45	21.66268391138431	27.80600427931737	28.39378543120372	22.137526378094602
46	22.592442813761462	27.221237766813967	29.33994103071256	20.846378388712015
47	21.71466805879282	26.721616434931583	30.42491709741697	21.138798408858626
48	22.159658242832876	25.64708131438277	30.873951309897574	21.319309132886772
49	22.025249443026993	25.034080130585355	30.64101527172195	22.299655154665707
50	20.54712028411138	26.353462791261954	31.17806224862687	21.921354675999794
51	20.504327110431387	27.09013771755035	30.198525032536043	22.207010139482218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1107.0
1	2208.5
2	3310.0
3	24473.0
4	45636.0
5	31031.0
6	16426.0
7	16126.5
8	15827.0
9	15782.0
10	15737.0
11	15264.0
12	14791.0
13	14249.0
14	13707.0
15	12877.0
16	12047.0
17	11391.5
18	10736.0
19	10407.0
20	10078.0
21	9898.0
22	9718.0
23	9799.5
24	9881.0
25	10450.5
26	12138.5
27	13257.0
28	14799.5
29	16342.0
30	18264.5
31	20187.0
32	23398.0
33	26609.0
34	29906.0
35	33203.0
36	36857.5
37	40512.0
38	46742.0
39	52972.0
40	59435.0
41	65898.0
42	70397.5
43	74897.0
44	79120.0
45	83343.0
46	88690.5
47	94038.0
48	94187.5
49	94337.0
50	92155.0
51	89973.0
52	84810.5
53	79648.0
54	75907.5
55	72167.0
56	70573.0
57	68979.0
58	66485.5
59	63992.0
60	59280.5
61	54569.0
62	48288.5
63	42008.0
64	35442.0
65	28876.0
66	23900.0
67	18924.0
68	15892.5
69	12861.0
70	11002.5
71	9144.0
72	7598.0
73	6052.0
74	4883.5
75	2800.5
76	1886.0
77	1371.0
78	856.0
79	668.5
80	481.0
81	306.5
82	132.0
83	105.0
84	78.0
85	55.0
86	32.0
87	21.5
88	11.0
89	15.5
90	20.0
91	14.0
92	8.0
93	4.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1360030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.088746287731766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29413634654331	24.30327725720271
2	6.94926026891407	4.737831403888528
3	3.757100162926171	3.8422450269475785
4	2.7231895143774585	3.7132046579609863
5	2.1425719827474854	3.651879636154072
6	1.7963427703133221	3.6741043765807238
7	1.5099701321622652	3.6031092116132584
8	1.2882875380305783	3.5132885623656773
9	1.1428902946922692	3.506372756142818
>10	7.320498274707958	40.49535334756901
>50	0.050807885331608266	1.1539241763094
>100	0.02341313024376732	1.5238201258954462
>500	8.752565770056799E-4	0.21198761766747887
>1k	2.1881414425141998E-4	0.09726666201210149
>5k	2.1881414425141998E-4	0.7010062036731057
>10k+	2.1881414425141998E-4	1.271328978017069
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17044	1.2532076498312537	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9398	0.6910141688050999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006249862135394072	0.0
2	0.0	0.0	0.0	0.03286692205319	0.0
3	0.0	0.0	0.0	0.05396939773387352	0.0
4	0.0	0.0	0.0	0.08301287471599891	0.0
5	0.0	0.0	0.0	0.1609523319338544	0.0
6	0.0	0.0	0.0	0.27933207355720097	0.0
7	0.0	0.0	0.0	0.35131577979897505	0.0
8	0.0	0.0	0.0	0.49837135945530614	0.0
9	0.0	0.0	0.0	0.8296875804210201	0.0
10	0.0	0.0	0.0	1.3565141945398262	0.0
11	0.0	0.0	0.0	1.6472430755203928	0.0
12	0.0	0.0	0.0	1.9282662882436417	0.0
13	0.0	0.0	0.0	2.0606163099343395	0.0
14	0.0	0.0	0.0	2.1342176275523332	0.0
15	0.0	0.0	0.0	2.199730888289229	0.0
16	0.0	0.0	0.0	2.2895818474592473	0.0
17	0.0	0.0	0.0	2.3861238355036285	0.0
18	0.0	0.0	0.0	2.534797026536179	0.0
19	0.0	0.0	0.0	2.6175893178826937	0.0
20	0.0	0.0	0.0	2.702146276185084	0.0
21	0.0	0.0	0.0	2.7884679014433504	0.0
22	0.0	0.0	0.0	2.8889803901384528	0.0
23	0.0	0.0	0.0	2.9958897965486058	0.0
24	0.0	0.0	0.0	3.088902450681235	0.0
25	0.0	0.0	0.0	3.166253685580465	0.0
26	0.0	0.0	0.0	3.2431637537407263	0.0
27	0.0	0.0	0.0	3.3310294625853842	0.0
28	0.0	0.0	0.0	3.421615699653684	0.0
29	0.0	0.0	0.0	3.5085255472305756	0.0
30	0.0	0.0	0.0	3.6326404564605195	0.0
31	0.0	0.0	0.0	3.7274177775490247	0.0
32	0.0	0.0	0.0	3.8551355484805483	0.0
33	0.0	0.0	0.0	3.971677095358191	0.0
34	0.0	0.0	0.0	4.077704168290405	0.0
35	0.0	0.0	0.0	4.197039771181518	0.0
36	0.0	0.0	0.0	4.313654845848989	0.0
37	0.0	0.0	0.0	4.427622920082645	0.0
38	0.0	0.0	0.0	4.5872517517995925	0.0
39	0.0	0.0	0.0	4.762468474960111	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	20	7.0337433E-4	45.0	14
CGTAAGA	80	0.0	45.0	2
ACGCGTA	25	3.891184E-5	45.0	15
CGTTTTT	14895	0.0	43.62538	1
TATAGCG	75	0.0	42.000004	1
GTTTTTA	10115	0.0	41.9303	2
CGGTCTA	245	0.0	39.489796	31
TATTTTT	11515	0.0	38.53235	7
TTATTTT	11515	0.0	38.47373	6
TCACGAC	235	0.0	38.29787	25
TTTTATT	11730	0.0	37.97954	4
CGACGGT	255	0.0	37.941177	28
TTTATTT	11715	0.0	37.932137	5
ACACGAC	405	0.0	37.777775	26
GAGCGAT	1125	0.0	37.6	7
CACGACG	240	0.0	37.499996	26
ACGACGG	240	0.0	37.499996	27
TCTCACG	255	0.0	37.058823	23
TTTTTAT	12035	0.0	36.96095	3
CGAGTAC	55	2.750312E-9	36.81818	6
>>END_MODULE