##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546897_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	840036
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60045045688518	31.0	31.0	34.0	30.0	34.0
2	31.92651148284121	31.0	31.0	34.0	30.0	34.0
3	31.78237242213429	31.0	31.0	34.0	30.0	34.0
4	35.65131018194458	37.0	35.0	37.0	33.0	37.0
5	35.67823164721512	37.0	35.0	37.0	33.0	37.0
6	35.74656681380322	37.0	35.0	37.0	35.0	37.0
7	35.96781090334224	37.0	35.0	37.0	35.0	37.0
8	13.370136517958754	0.0	0.0	35.0	0.0	37.0
9	24.90966101452795	18.0	17.0	38.0	17.0	39.0
10	32.50520691970344	32.0	32.0	37.0	27.0	39.0
11	35.41386916751187	37.0	34.0	37.0	32.0	39.0
12	36.60022427610245	37.0	35.0	39.0	33.0	39.0
13	36.88873333999972	39.0	37.0	39.0	33.0	39.0
14	38.1267850425458	40.0	37.0	41.0	33.0	41.0
15	38.33710698112938	40.0	37.0	41.0	33.0	41.0
16	38.413301334704705	40.0	38.0	41.0	34.0	41.0
17	38.337134360908344	40.0	37.0	41.0	33.0	41.0
18	38.23635772752596	40.0	37.0	41.0	33.0	41.0
19	38.16849515973125	40.0	37.0	41.0	34.0	41.0
20	38.01004599802866	40.0	36.0	41.0	33.0	41.0
21	37.99132180049426	40.0	36.0	41.0	33.0	41.0
22	37.94843078153794	40.0	36.0	41.0	33.0	41.0
23	37.70280559404597	39.0	36.0	41.0	33.0	41.0
24	37.384664466760945	39.0	35.0	41.0	33.0	41.0
25	37.289182844544754	39.0	35.0	41.0	33.0	41.0
26	37.18834430905342	39.0	35.0	41.0	33.0	41.0
27	37.0943828597822	39.0	35.0	41.0	33.0	41.0
28	36.94352027770238	39.0	35.0	41.0	32.0	41.0
29	36.824324195629714	39.0	35.0	41.0	32.0	41.0
30	36.6186115833131	39.0	35.0	41.0	31.0	41.0
31	36.41165616711665	39.0	35.0	41.0	31.0	41.0
32	35.99614659371741	39.0	35.0	41.0	29.0	41.0
33	35.5391852253951	39.0	35.0	41.0	25.0	41.0
34	35.02248356022837	38.0	35.0	41.0	22.0	41.0
35	34.65697422491417	38.0	34.0	41.0	19.0	41.0
36	34.47397611530934	38.0	34.0	40.0	18.0	41.0
37	34.36986510101948	38.0	34.0	40.0	18.0	41.0
38	34.268214695560665	38.0	34.0	40.0	18.0	41.0
39	34.14334504711703	38.0	34.0	40.0	15.0	41.0
40	34.05782847401778	38.0	33.0	40.0	15.0	41.0
41	33.96398011513792	38.0	33.0	40.0	15.0	41.0
42	33.88018965853844	38.0	33.0	40.0	15.0	41.0
43	33.76435533715222	38.0	33.0	40.0	15.0	41.0
44	33.61180949387883	38.0	33.0	40.0	14.0	41.0
45	33.54522544271912	38.0	33.0	40.0	15.0	41.0
46	33.486407725383195	37.0	33.0	40.0	15.0	41.0
47	33.38337523629939	37.0	33.0	40.0	13.0	41.0
48	33.31643048631249	37.0	33.0	40.0	13.0	41.0
49	33.210713588465254	37.0	33.0	40.0	12.0	41.0
50	33.081310800965674	37.0	33.0	40.0	12.0	41.0
51	32.77102647981753	36.0	32.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	5.0
15	13.0
16	56.0
17	168.0
18	404.0
19	898.0
20	1905.0
21	3413.0
22	5481.0
23	8400.0
24	12714.0
25	18564.0
26	22822.0
27	22303.0
28	20167.0
29	19167.0
30	20071.0
31	23554.0
32	29741.0
33	39487.0
34	56754.0
35	85456.0
36	87769.0
37	135666.0
38	156105.0
39	68939.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51360655971887	20.129970620306747	24.07384921598598	23.282573603988403
2	41.42715312200906	23.468518015894556	23.60255989029042	11.50176897180597
3	26.278278549966906	24.180630353937214	37.875162493036015	11.66592860305987
4	23.66993795504002	23.539467356160927	39.26986462484941	13.52073006394964
5	22.34344718559681	27.20514358908428	36.49248365546239	13.95892556985653
6	18.560871200758065	37.33589989000471	34.841602026579814	9.26162688265741
7	74.4251436843183	4.091134189487118	19.447261784018778	2.0364603421758116
8	28.015108876286256	63.90071377893328	6.74268721816684	1.3414901266136214
9	69.34964691989391	5.678923284240199	19.543210052902495	5.428219742963396
10	36.82294568328024	25.15582665504812	25.528667818998237	12.492559842673408
11	29.917527344066208	22.572127861186903	32.43039584017828	15.079948954568614
12	25.876152926779326	20.897437728859238	36.98424829412073	16.242161050240703
13	21.62323995638282	25.917103552704884	37.420539119752014	15.039117371160286
14	16.978796146831804	30.92081767924232	37.29578256169974	14.804603612226142
15	15.922174763938687	23.984448285549668	44.534400906627816	15.558976043883835
16	18.384926360298845	22.48284597326781	41.728568775623906	17.403658890809442
17	18.33742839592589	22.637482203143673	36.434152822021915	22.590936578908526
18	20.50138327405016	23.63517754001019	37.56565194824984	18.297787237689814
19	22.778904713607513	25.247965563380614	33.82843116247399	18.14469856053788
20	24.845601855158588	24.131941964392002	33.342142479608015	17.680313700841392
21	21.602645600902818	26.32637172692599	35.57383255003357	16.497150122137622
22	20.58042750548786	22.767357589436642	35.83834502330853	20.813869881766973
23	19.886885800132376	26.74647277021461	34.590184230199654	18.776457199453354
24	19.671180758919856	25.682351708736288	36.94782128384974	17.698646248494114
25	19.438095510192422	26.08471541695832	36.030717731144854	18.446471341704402
26	17.58615106971606	27.096695855891888	35.32991443223862	19.987238642153432
27	19.399287649576923	26.334585660614547	35.80156088548586	18.46456580432267
28	17.541748210790967	26.028051178759004	38.88964282483131	17.540557785618713
29	19.44202391326086	25.3348666009552	37.911232375755326	17.311877110028618
30	19.743677651910158	25.904604088396212	37.10305272631173	17.248665533381903
31	24.556447580817963	24.106466865705755	32.63074439666872	18.706341156807564
32	25.923650891152285	25.600331414367954	32.17707336352252	16.298944330957244
33	23.74410144327148	25.07773476374822	31.607693003633173	19.570470789347123
34	20.119137751239233	27.647386540576836	32.66883800218086	19.564637706003076
35	20.534477093838834	28.011894728321167	32.30921055764277	19.14441762019723
36	24.20658162269236	27.954635277535722	29.620159136037028	18.21862396373489
37	21.016123118533013	29.622063816312632	30.448933140960627	18.912879924193724
38	20.17627815950745	31.529839197367732	27.850473074963457	20.443409568161364
39	20.647924612754693	29.301601359941714	28.642343899547164	21.40813012775643
40	21.103619368693725	27.3825169397502	29.90098043417187	21.61288325738421
41	18.61479746106119	27.282164097729144	30.302867972324997	23.800170468884666
42	20.838868810384316	28.782814069873197	28.78340928245932	21.594907837283163
43	21.29587303401283	27.645124732749547	28.740673018775386	22.31832921446224
44	20.951125904127917	28.756148546014693	30.204657895613995	20.08806765424339
45	20.584474951073524	29.672061673547322	28.83269288459066	20.91077049078849
46	22.516773090677066	29.40826345537572	28.908999138132174	19.16596431581504
47	20.489717107362065	28.78198077225262	30.394768795623044	20.33353332476227
48	21.402297044412382	27.814046064692466	30.30929626825517	20.47436062263998
49	20.93267431395797	26.728735435148014	31.564004399811434	20.774585851082573
50	19.329885862034484	28.536157974182057	31.658881286040124	20.475074877743335
51	19.849625492240808	29.072801641834396	30.102281330800107	20.975291535124686
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	644.0
1	1209.5
2	1775.0
3	18909.5
4	36044.0
5	23855.0
6	11666.0
7	11643.5
8	11621.0
9	12027.0
10	12433.0
11	12742.5
12	13052.0
13	12543.0
14	12034.0
15	11468.5
16	10903.0
17	10311.0
18	9719.0
19	9074.5
20	8430.0
21	7964.5
22	7499.0
23	7314.5
24	7130.0
25	7478.5
26	8759.0
27	9691.0
28	10660.5
29	11630.0
30	13019.0
31	14408.0
32	16215.5
33	18023.0
34	19727.0
35	21431.0
36	23371.0
37	25311.0
38	29884.5
39	34458.0
40	39779.5
41	45101.0
42	47591.0
43	50081.0
44	51608.0
45	53135.0
46	57191.5
47	61248.0
48	65259.5
49	69271.0
50	63133.0
51	56995.0
52	52264.0
53	47533.0
54	43393.0
55	39253.0
56	36442.0
57	33631.0
58	30531.5
59	27432.0
60	24642.0
61	21852.0
62	19342.5
63	16833.0
64	14002.5
65	11172.0
66	9366.5
67	7561.0
68	6386.5
69	5212.0
70	4248.0
71	3284.0
72	2779.0
73	2274.0
74	1786.0
75	950.5
76	603.0
77	447.5
78	292.0
79	211.5
80	131.0
81	95.5
82	60.0
83	41.5
84	23.0
85	18.5
86	14.0
87	10.5
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.213072254898755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68215159761375	32.577142924817004
2	8.836004984023502	7.813338536065531
3	4.494713089300757	5.961752237468808
4	2.84966192771872	5.039692348490473
5	2.066815349736654	4.569012819772027
6	1.5858640132398265	4.20695521222897
7	1.2225114635562835	3.783569136945919
8	0.9858053164374034	3.4868385347928195
9	0.7879857306699729	3.135534304134663
>10	3.4359609496863093	22.510826023846953
>50	0.027744482315261228	0.8235329582089864
>100	0.02208749834309105	2.003369234774921
>500	0.0010774389433910454	0.29951691980947753
>1k	0.0010774389433910454	0.8666340459059911
>5k	2.6935973584776135E-4	1.0391986649135192
>10k+	2.6935973584776135E-4	1.8830860978240394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15812	1.8823002823688508	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8726	1.0387650053092963	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	2288	0.27236927941183475	No Hit
GAATCTGACTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	2276	0.27094076920512933	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	1653	0.19677728097367256	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	1060	0.12618506825897938	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0077377636196543954	0.0
2	0.0	0.0	0.0	0.05083115485526811	0.0
3	0.0	0.0	0.0	0.09047231309134371	0.0
4	0.0	0.0	0.0	0.1752305853558657	0.0
5	0.0	0.0	0.0	0.4637896471103619	0.0
6	0.0	0.0	0.0	0.6724711798065797	0.0
7	0.0	0.0	0.0	0.8380593212671837	0.0
8	0.0	0.0	0.0	1.0693589322362376	0.0
9	0.0	0.0	0.0	1.4602945588046226	0.0
10	0.0	0.0	0.0	2.209310077187168	0.0
11	0.0	0.0	0.0	2.473346380393221	0.0
12	0.0	0.0	0.0	2.8670199848577917	0.0
13	0.0	0.0	0.0	3.025822702836545	0.0
14	0.0	0.0	0.0	3.123199481926965	0.0
15	0.0	0.0	0.0	3.206291158950331	0.0
16	0.0	0.0	0.0	3.3112866591431795	0.0
17	0.0	0.0	0.0	3.4117585436814615	0.0
18	0.0	0.0	0.0	3.6097262498273883	0.0
19	0.0	0.0	0.0	3.710674304434572	0.0
20	0.0	0.0	0.0	3.8026941702498465	0.0
21	0.0	0.0	0.0	3.898642439133561	0.0
22	0.0	0.0	0.0	4.003399854291959	0.0
23	0.0	0.0	0.0	4.1062525891747494	0.0
24	0.0	0.0	0.0	4.191487031508173	0.0
25	0.0	0.0	0.0	4.263150626877896	0.0
26	0.0	0.0	0.0	4.337790285178254	0.0
27	0.0	0.0	0.0	4.433619511544744	0.0
28	0.0	0.0	0.0	4.527901185187302	0.0
29	0.0	0.0	0.0	4.620278178554252	0.0
30	0.0	0.0	0.0	4.750867819950574	0.0
31	0.0	0.0	0.0	4.847173216385964	0.0
32	0.0	0.0	0.0	4.965144350956388	0.0
33	0.0	0.0	0.0	5.080615592665076	0.0
34	0.0	0.0	0.0	5.197158217028794	0.0
35	0.0	0.0	0.0	5.325009880528929	0.0
36	0.0	0.0	0.0	5.43833835692756	0.0
37	0.0	0.0	0.0	5.542381516982606	0.0
38	0.0	0.0	0.0	5.6780899866196215	0.0
39	1.1904251722545224E-4	0.0	0.0	5.8493921689070465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGCGA	35	1.2114106E-7	45.000004	2
CGACGGT	110	0.0	45.0	28
CGCGCGA	20	7.032202E-4	45.0	2
TAAGTCG	20	7.032202E-4	45.0	1
TCGGCGA	20	7.032202E-4	45.0	8
CGAACCC	30	2.1648011E-6	44.999996	34
CGTTTTT	11810	0.0	44.028366	1
AGCGATA	145	0.0	43.44828	8
GTTTTTA	7780	0.0	43.17802	2
CGAGATC	80	0.0	42.1875	6
GAGCGAT	705	0.0	41.48936	7
CGAATGC	125	0.0	41.399998	45
CGCATCG	60	3.6379788E-12	41.249996	21
CACGACG	120	0.0	41.249996	26
CGGTCTA	120	0.0	41.249996	31
ATTGCGA	60	3.6379788E-12	41.249996	2
GTTACGA	50	1.0804797E-9	40.5	2
TATTTTT	8515	0.0	40.296535	7
ACCGCTC	95	0.0	40.26316	18
CCGACCG	95	0.0	40.26316	15
>>END_MODULE