FastQCFastQC Report
Thu 26 May 2016
SRR1546896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546896_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1114118
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100400.9011612773512321No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.5003958288080795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12810.1149788442516861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGC406.8139343E-945.021
ATACGCG207.033193E-445.01
GTTGCGA302.1654414E-644.9999962
CGTTTTT75550.043.2428861
CGGTCTA3200.042.187531
GTTTTTA53600.041.8936542
TAGAGTA3600.041.255
CACGACG3200.040.07812526
CGACGGT3350.039.6268628
TCACGAC3300.039.54545625
GCGATAC403.458099E-739.3759
ACTCGAT403.458099E-739.37514
CTTGCGA750.039.02
GAGCGAT9700.038.2731977
CGCATGA659.094947E-1238.0769272
AGGGGAA61100.037.671858
TATTTTT63100.037.5475437
TTTACGA601.5643309E-1037.4999962
TTTTATT64300.037.4766734
GCGCGAC4750.037.421059
Produced by FastQC (version 0.11.5)