##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546896_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1114118
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.694815091399654	31.0	31.0	34.0	30.0	34.0
2	32.04368029239273	31.0	31.0	34.0	30.0	34.0
3	32.027352578452195	33.0	31.0	34.0	30.0	34.0
4	35.81272540251571	37.0	35.0	37.0	35.0	37.0
5	35.813287281957564	37.0	35.0	37.0	35.0	37.0
6	35.86012253639202	37.0	35.0	37.0	35.0	37.0
7	36.067531446399755	37.0	35.0	37.0	35.0	37.0
8	13.29995117213796	0.0	0.0	35.0	0.0	37.0
9	24.93222261914806	18.0	17.0	39.0	17.0	39.0
10	32.59916543849036	32.0	32.0	37.0	27.0	39.0
11	35.509260239938676	37.0	34.0	37.0	32.0	39.0
12	36.62839932574467	37.0	35.0	39.0	33.0	39.0
13	36.904965183221165	39.0	37.0	39.0	33.0	39.0
14	38.07723329126717	40.0	37.0	41.0	33.0	41.0
15	38.34546609964115	40.0	37.0	41.0	34.0	41.0
16	38.42720789000806	40.0	38.0	41.0	34.0	41.0
17	38.38670320378991	40.0	37.0	41.0	34.0	41.0
18	38.323489971439294	40.0	37.0	41.0	34.0	41.0
19	38.29723332717001	40.0	37.0	41.0	34.0	41.0
20	38.2331934319345	40.0	37.0	41.0	34.0	41.0
21	38.16472043356269	40.0	37.0	41.0	34.0	41.0
22	38.12363591648281	40.0	37.0	41.0	33.0	41.0
23	37.98256378588265	40.0	36.0	41.0	33.0	41.0
24	37.807496153908296	40.0	35.0	41.0	33.0	41.0
25	37.69412575687674	40.0	35.0	41.0	33.0	41.0
26	37.62010576976586	40.0	35.0	41.0	33.0	41.0
27	37.56259480593618	40.0	35.0	41.0	33.0	41.0
28	37.44376807483588	40.0	35.0	41.0	33.0	41.0
29	37.381407534928975	40.0	35.0	41.0	33.0	41.0
30	37.25868893600139	40.0	35.0	41.0	33.0	41.0
31	37.14794124141249	40.0	35.0	41.0	32.0	41.0
32	36.956265853347674	40.0	35.0	41.0	31.0	41.0
33	36.72674348677609	40.0	35.0	41.0	31.0	41.0
34	36.46824483582529	40.0	35.0	41.0	30.0	41.0
35	36.270413905887885	40.0	35.0	41.0	30.0	41.0
36	36.12940101497328	39.0	35.0	41.0	29.0	41.0
37	36.05951075200293	39.0	35.0	41.0	29.0	41.0
38	35.95272044792383	39.0	35.0	41.0	28.0	41.0
39	35.859699780454136	39.0	35.0	41.0	27.0	41.0
40	35.74676919320934	39.0	35.0	41.0	27.0	41.0
41	35.64049499245143	39.0	35.0	41.0	26.0	41.0
42	35.588349708020154	39.0	35.0	40.0	26.0	41.0
43	35.480193300889134	39.0	35.0	40.0	26.0	41.0
44	35.299280686605904	38.0	35.0	40.0	25.0	41.0
45	35.21270906672363	38.0	35.0	40.0	24.0	41.0
46	35.18609249648601	38.0	35.0	40.0	25.0	41.0
47	35.13524330456917	38.0	34.0	40.0	24.0	41.0
48	35.05009792499538	38.0	34.0	40.0	24.0	41.0
49	34.92833793188872	38.0	34.0	40.0	24.0	41.0
50	34.81408432499968	38.0	34.0	40.0	24.0	41.0
51	34.4712382350882	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	5.0
14	7.0
15	21.0
16	66.0
17	140.0
18	374.0
19	793.0
20	1415.0
21	2521.0
22	4062.0
23	6234.0
24	9819.0
25	14298.0
26	18064.0
27	19496.0
28	19408.0
29	20107.0
30	23549.0
31	28562.0
32	37244.0
33	50684.0
34	77022.0
35	111709.0
36	120671.0
37	200242.0
38	242749.0
39	104835.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.164154963836864	21.918234872787263	26.42951644260303	17.48809372077284
2	36.09375308540029	23.233266135184962	27.875593070033872	12.797387709380873
3	29.54417754672306	23.702067465026147	33.56700098194267	13.18675400630813
4	26.382573479649373	25.888729919092953	32.331045724061546	15.397650877196131
5	22.833936800231214	30.45251939202131	31.76279352815411	14.950750279593365
6	21.15323511513143	38.80127598692418	29.46599911319986	10.579489784744524
7	82.40608265910792	4.596999599683337	10.862224647658508	2.134693093550234
8	31.26473138392881	63.507994664838016	4.08843587483552	1.1388380763976527
9	77.97692883518621	5.595188301418701	12.794694996400741	3.63318786699434
10	41.907679437905145	26.883328336854806	19.001577929806356	12.207414295433697
11	35.09457705557221	22.340811296469496	26.597182704165984	15.967428943792308
12	30.73031761447172	21.369459967436125	30.72197020423331	17.178252213858855
13	23.995483422761325	27.014283944788613	31.731827328882577	17.258405303567486
14	18.850516731620885	30.11736638309407	33.69427654880363	17.337840336481413
15	18.42084949709097	23.219982084482975	41.06315489023604	17.296013528190013
16	22.13463923929063	21.068683927555252	38.38202057591745	18.41465625723667
17	21.882242275952816	20.77212647134325	31.725454574829598	25.620176677874333
18	23.747933342787746	22.354005590072145	34.023595346273915	19.87446572086619
19	26.675181623490513	23.849897407635456	29.23263065492165	20.24229031395238
20	26.898766557940903	24.65546737419196	28.356332094086984	20.089433973780157
21	25.002827348629143	24.965578152403964	31.76476818433954	18.266826314627355
22	23.677563776906936	21.934391150668063	31.33088236614075	23.057162706284252
23	21.341635266641415	26.07192415884134	30.826178196564456	21.760262377952785
24	21.80702582670776	24.270858203529606	34.18686350996932	19.73525245979331
25	22.321513520111875	23.786349381304316	32.68486820965104	21.20726888893277
26	19.838832152429095	26.98134308933165	30.136574402352355	23.043250355886897
27	20.265896431078218	25.861533517993607	32.5317425981808	21.340827452747373
28	19.378288475726986	25.469833536483566	34.99934477317483	20.15253321461461
29	20.43131876515773	25.28637002543716	33.91480974187653	20.367501467528573
30	22.235167190548935	25.003096619927152	33.06669491023392	19.695041279289985
31	26.585155252854726	23.82754788990035	28.987055231133507	20.60024162611142
32	26.704891223371312	24.980388073794696	28.943074252458	19.371646450375994
33	25.507172489808084	24.647568749450237	29.067387835040815	20.777870925700867
34	21.454639454707667	26.312383427967234	30.639662944140568	21.593314173184528
35	21.751735453515696	26.971828836801848	30.257656729359006	21.01877898032345
36	26.15468020443077	26.727420255305095	27.272963905080072	19.844935635184065
37	22.118393204310493	29.503966366219736	27.678665994086803	20.698974435382965
38	22.932669609502764	30.29418786878948	25.57332347202002	21.199819049687736
39	22.19351989645621	27.473032479503967	27.13931558416613	23.194132039873693
40	23.72854580933079	24.97697730401986	28.06156977986174	23.23290710678761
41	20.601857253899496	24.6070882976489	29.36008573598129	25.430968712470314
42	22.416117502813883	26.33482270280168	26.928745429119715	24.32031436526472
43	22.106186238800557	25.62924214490745	27.875323798735863	24.38924781755613
44	21.751376425118345	26.836116102603135	28.636284486921493	22.776222985357027
45	21.10494579568771	28.645619225252624	27.42644854494766	22.822986434112007
46	22.705853419476213	27.427525630139716	28.572108160894988	21.294512789489083
47	21.687379613290513	27.038338847411136	28.68277866437846	22.591502874919893
48	22.023160921913117	26.186992760192368	29.77934114698802	22.010505170906494
49	22.575436354138432	24.59344521854956	30.864145449584335	21.96697297772767
50	21.398271996323547	27.81895634035174	28.94702356482886	21.83574809849585
51	20.7145024135684	28.723079601981116	28.298528522113457	22.263889462337026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	856.0
1	1643.5
2	2431.0
3	13735.0
4	25039.0
5	16706.5
6	8374.0
7	8293.0
8	8212.0
9	8208.5
10	8205.0
11	8026.5
12	7848.0
13	7594.5
14	7341.0
15	6916.5
16	6492.0
17	6283.0
18	6074.0
19	5987.0
20	5900.0
21	6112.5
22	6325.0
23	6557.5
24	6790.0
25	7699.0
26	10193.0
27	11778.0
28	13368.5
29	14959.0
30	17093.0
31	19227.0
32	21376.0
33	23525.0
34	25595.5
35	27666.0
36	31473.0
37	35280.0
38	41670.0
39	48060.0
40	55985.5
41	63911.0
42	69955.5
43	76000.0
44	78534.0
45	81068.0
46	82019.5
47	82971.0
48	80858.0
49	78745.0
50	75813.5
51	72882.0
52	69582.0
53	66282.0
54	64130.0
55	61978.0
56	59511.5
57	57045.0
58	53165.5
59	49286.0
60	44310.5
61	39335.0
62	34929.0
63	30523.0
64	26115.5
65	21708.0
66	18185.0
67	14662.0
68	12832.5
69	11003.0
70	9292.0
71	7581.0
72	6222.5
73	4864.0
74	3764.5
75	2025.0
76	1385.0
77	1039.5
78	694.0
79	495.5
80	297.0
81	220.5
82	144.0
83	103.0
84	62.0
85	38.5
86	15.0
87	10.5
88	6.0
89	6.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1114118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.1505491881501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.09728019844917	21.835687747773306
2	7.047160144246653	4.390458174202527
3	3.6705839763658625	3.430221201150612
4	2.6073327507748716	3.2487938841154933
5	2.109961934140503	3.2863236507284026
6	1.7563077915265173	3.282597134968683
7	1.5326036613807208	3.3419012017845353
8	1.30932725756838	3.2629010512215597
9	1.1306001891135762	3.169693512280283
>10	8.650162300423963	46.356398760926496
>50	0.062394607890409216	1.2715340891699705
>100	0.023974402357977296	1.354255974478417
>500	0.0014442411009015189	0.24896859170990263
>1k	2.8884822018030375E-4	0.11526157064704924
>5k	2.8884822018030375E-4	0.5016262735029661
>10k+	2.8884822018030375E-4	0.9033771813398708
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10040	0.9011612773512321	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5575	0.5003958288080795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1281	0.1149788442516861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7951419867554425E-4	0.0	0.0	0.00421858366887529	0.0
2	1.7951419867554425E-4	0.0	0.0	0.0164255491788123	0.0
3	1.7951419867554425E-4	0.0	0.0	0.03276134125828682	0.0
4	1.7951419867554425E-4	0.0	0.0	0.052328388913921144	0.0
5	1.7951419867554425E-4	0.0	0.0	0.09972013736426483	0.0
6	1.7951419867554425E-4	0.0	0.0	0.1983631895364764	0.0
7	1.7951419867554425E-4	0.0	0.0	0.2616419445696057	0.0
8	1.7951419867554425E-4	0.0	0.0	0.4160241554305738	0.0
9	1.7951419867554425E-4	0.0	0.0	0.6407759321723552	0.0
10	1.7951419867554425E-4	0.0	0.0	0.9477452119075358	0.0
11	1.7951419867554425E-4	0.0	0.0	1.1363248776161952	0.0
12	1.7951419867554425E-4	0.0	0.0	1.3122487923182284	0.0
13	1.7951419867554425E-4	0.0	0.0	1.4237271096957413	0.0
14	1.7951419867554425E-4	0.0	0.0	1.48341558075536	0.0
15	1.7951419867554425E-4	0.0	0.0	1.5246140893513973	0.0
16	1.7951419867554425E-4	0.0	0.0	1.5901367718679709	0.0
17	1.7951419867554425E-4	0.0	0.0	1.659429252556731	0.0
18	1.7951419867554425E-4	0.0	0.0	1.78356332094087	0.0
19	1.7951419867554425E-4	0.0	0.0	1.8406488361196929	0.0
20	1.7951419867554425E-4	0.0	0.0	1.9113774303978572	0.0
21	1.7951419867554425E-4	0.0	0.0	1.9856963086495327	0.0
22	1.7951419867554425E-4	0.0	0.0	2.0680035687422698	0.0
23	1.7951419867554425E-4	0.0	0.0	2.1636846366363347	0.0
24	1.7951419867554425E-4	0.0	0.0	2.2337849312191347	0.0
25	1.7951419867554425E-4	0.0	0.0	2.2982305285436553	0.0
26	1.7951419867554425E-4	0.0	0.0	2.3615990406761225	0.0
27	1.7951419867554425E-4	0.0	0.0	2.434033019841704	0.0
28	1.7951419867554425E-4	0.0	0.0	2.512211453364904	0.0
29	1.7951419867554425E-4	0.0	0.0	2.6012504959079736	0.0
30	1.7951419867554425E-4	0.0	0.0	2.7129083274841626	0.0
31	1.7951419867554425E-4	0.0	0.0	2.803203969417961	0.0
32	1.7951419867554425E-4	0.0	0.0	2.905706576861697	0.0
33	1.7951419867554425E-4	0.0	0.0	3.014312667060401	0.0
34	1.7951419867554425E-4	0.0	0.0	3.131445681696194	0.0
35	1.7951419867554425E-4	0.0	0.0	3.2576441633651014	0.0
36	1.7951419867554425E-4	0.0	0.0	3.3635575405836726	0.0
37	1.7951419867554425E-4	0.0	0.0	3.4829344827029094	0.0
38	1.7951419867554425E-4	0.0	0.0	3.6403684349413616	0.0
39	1.7951419867554425E-4	0.0	0.0	3.9178076289944155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGC	40	6.8139343E-9	45.0	21
ATACGCG	20	7.033193E-4	45.0	1
GTTGCGA	30	2.1654414E-6	44.999996	2
CGTTTTT	7555	0.0	43.242886	1
CGGTCTA	320	0.0	42.1875	31
GTTTTTA	5360	0.0	41.893654	2
TAGAGTA	360	0.0	41.25	5
CACGACG	320	0.0	40.078125	26
CGACGGT	335	0.0	39.62686	28
TCACGAC	330	0.0	39.545456	25
GCGATAC	40	3.458099E-7	39.375	9
ACTCGAT	40	3.458099E-7	39.375	14
CTTGCGA	75	0.0	39.0	2
GAGCGAT	970	0.0	38.273197	7
CGCATGA	65	9.094947E-12	38.076927	2
AGGGGAA	6110	0.0	37.67185	8
TATTTTT	6310	0.0	37.547543	7
TTTACGA	60	1.5643309E-10	37.499996	2
TTTTATT	6430	0.0	37.476673	4
GCGCGAC	475	0.0	37.42105	9
>>END_MODULE