FastQCFastQC Report
Thu 26 May 2016
SRR1546895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546895_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1138126
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220821.9402069718115569No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124911.097505900049731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT186550.044.1919061
GTTTTTA127150.043.0357862
TCACGAC1800.041.25000425
TAGCCGT1050.040.71428744
GTAGCCG1050.040.71428743
CACGACC4900.040.40816527
TTATTTT140100.040.2301946
TATTTTT140000.040.1946457
CACGACG1850.040.13513626
CGGTCTA1850.040.13513631
AGACTTA8900.039.691018
TTTATTT142300.039.5607875
TTTGCGA1950.039.230772
TTTTATT143950.039.1854824
CGCCGTT750.039.00000426
ACACGAC4950.038.63636426
ACGACCA5100.038.38235528
CGACGGT1950.038.07692328
TTTTTAT148450.037.8612333
TAGAGGC5000.037.355