##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546895_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1138126
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.701654298381726	31.0	31.0	34.0	30.0	34.0
2	32.052010058640256	33.0	31.0	34.0	30.0	34.0
3	31.87891498832291	31.0	31.0	34.0	30.0	34.0
4	35.75332432437182	37.0	35.0	37.0	35.0	37.0
5	35.810347009030636	37.0	35.0	37.0	35.0	37.0
6	35.86709204429035	37.0	35.0	37.0	35.0	37.0
7	36.11022593280533	37.0	35.0	37.0	35.0	37.0
8	13.252113562118781	0.0	0.0	36.0	0.0	37.0
9	24.925739329388836	18.0	17.0	39.0	17.0	39.0
10	32.67050660471688	32.0	32.0	38.0	27.0	39.0
11	35.59972885251721	37.0	35.0	37.0	32.0	39.0
12	36.81410054774252	39.0	35.0	39.0	33.0	39.0
13	37.16048750314113	39.0	37.0	39.0	34.0	39.0
14	38.402189212793665	40.0	38.0	41.0	34.0	41.0
15	38.59460024636991	40.0	38.0	41.0	34.0	41.0
16	38.620695775335946	40.0	38.0	41.0	34.0	41.0
17	38.56475908642804	40.0	38.0	41.0	34.0	41.0
18	38.44032910240167	40.0	38.0	41.0	34.0	41.0
19	38.34245856785628	40.0	37.0	41.0	34.0	41.0
20	38.24671521430843	40.0	37.0	41.0	34.0	41.0
21	38.190275066205324	40.0	37.0	41.0	34.0	41.0
22	38.147788557681665	40.0	36.0	41.0	34.0	41.0
23	37.88920295292437	39.0	36.0	41.0	33.0	41.0
24	37.45869438005985	39.0	35.0	41.0	33.0	41.0
25	37.33749426689136	39.0	35.0	41.0	33.0	41.0
26	37.27088125567819	39.0	35.0	41.0	33.0	41.0
27	37.22336191247718	39.0	35.0	41.0	33.0	41.0
28	37.059851018252814	39.0	35.0	41.0	33.0	41.0
29	36.925177001491924	39.0	35.0	41.0	33.0	41.0
30	36.720660102659984	39.0	35.0	41.0	32.0	41.0
31	36.48729666135384	39.0	35.0	41.0	31.0	41.0
32	36.06189736461516	39.0	35.0	41.0	30.0	41.0
33	35.553153165818195	39.0	35.0	41.0	25.0	41.0
34	35.02090014638098	39.0	35.0	41.0	21.0	41.0
35	34.61626304996108	39.0	34.0	41.0	18.0	41.0
36	34.38645545396555	39.0	34.0	41.0	16.0	41.0
37	34.30711274498606	39.0	34.0	41.0	15.0	41.0
38	34.152397889161655	38.0	34.0	40.0	15.0	41.0
39	34.01694276380647	38.0	34.0	40.0	15.0	41.0
40	33.87859077114485	38.0	33.0	40.0	15.0	41.0
41	33.751726961689656	38.0	33.0	40.0	12.0	41.0
42	33.65469640444028	38.0	33.0	40.0	12.0	41.0
43	33.53386268304212	38.0	33.0	40.0	12.0	41.0
44	33.278241600666355	38.0	33.0	40.0	10.0	41.0
45	33.212646930129004	37.0	33.0	40.0	10.0	41.0
46	33.169802816208396	37.0	33.0	40.0	10.0	41.0
47	33.14148609204956	37.0	33.0	40.0	10.0	41.0
48	33.04717228145214	37.0	32.0	40.0	10.0	41.0
49	32.93926595122157	37.0	32.0	40.0	10.0	41.0
50	32.848299748885445	36.0	32.0	40.0	10.0	41.0
51	32.52924280791406	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	13.0
15	28.0
16	61.0
17	199.0
18	501.0
19	1089.0
20	2073.0
21	3475.0
22	5837.0
23	9579.0
24	16003.0
25	25556.0
26	34022.0
27	35346.0
28	31840.0
29	28303.0
30	28117.0
31	31025.0
32	38429.0
33	50081.0
34	71378.0
35	103950.0
36	118177.0
37	189547.0
38	215957.0
39	97506.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.489089960162584	19.42904388442053	24.07387231290736	27.00799384250953
2	43.97948908996016	20.485868875678086	24.498693466277018	11.035948568084729
3	26.364479855481733	21.746274138364292	40.260744416699026	11.628501589454947
4	23.288106940707795	23.383351228247136	39.77916329123489	13.549378539810178
5	20.784605570912184	27.200503283467736	38.92389770552646	13.090993440093628
6	18.570263749356396	35.01536736705778	36.604646585703165	9.80972229788266
7	74.78196614434606	3.3374160681681997	20.055424443339316	1.82519334414643
8	28.16902522216345	63.50729181127572	7.338642645893337	0.9850403206674833
9	71.00672509019212	4.2194800927138125	21.522309480672615	3.251485336421451
10	34.15895955280874	23.342758183188856	28.14872869963431	14.349553564368092
11	27.97449491532572	23.233455698226734	33.251678636636015	15.540370749811533
12	24.493773097179048	20.19003168366244	38.0342773998661	17.281917819292417
13	22.02436285613368	20.4903499261066	38.71645142980654	18.76883578795318
14	18.76699064954144	23.665745268977247	39.828630573416305	17.738633508065014
15	18.744146078729422	21.722990248882812	41.85274741109508	17.680116261292685
16	21.87156782289483	20.27332650339242	40.564840799700555	17.29026487401219
17	21.090195637389886	20.4223433960739	38.544941421248616	19.942519545287603
18	22.679386992301378	21.367669309022023	37.962141274340446	17.990802424336145
19	23.47059991600227	23.575509214269776	35.30953514812947	17.644355721598487
20	24.789434561726907	22.62587797836092	34.928557997972106	17.65612946194007
21	23.45610239991003	22.92224235277992	36.748391654351096	16.87326359295895
22	21.798553060030258	19.918181290999414	37.99869258763968	20.284573061330644
23	21.234028569771713	23.283450162811498	36.828611243394846	18.653910024021943
24	21.167339995747394	22.532654556701104	37.836144679938776	18.463860767612726
25	22.119782871140806	21.712007282146264	36.46661265975823	19.701597186954693
26	20.030383279179986	22.944823332390264	36.370226143678295	20.65456724475146
27	20.104891725520723	21.469503376603292	37.82472239453277	20.600882503343215
28	19.278006125859527	23.679979193867815	38.15632012624262	18.885694554030046
29	21.1730511384504	23.69930921532414	36.908040058833556	18.219599587391905
30	21.790645323979945	21.556752064358427	38.27036725283492	18.382235358826705
31	24.06842476140603	21.695664627642284	35.35750874683471	18.878401864116977
32	23.79938600822756	23.099463504040855	35.051040043018084	18.050110444713503
33	22.90642688067929	23.108601332365662	33.944835633313005	20.04013615364204
34	21.574412674870796	25.16654570759301	34.92952449904492	18.329517118491275
35	21.40738371674138	25.294211712938637	33.59935543164816	19.699049138671818
36	23.10051786884756	27.35558277378779	30.69238379581874	18.85151556154591
37	23.178101545874537	26.83279355712812	29.839402667191507	20.14970222980584
38	22.91679480127859	27.746224934673315	29.446388185490886	19.890592078557205
39	23.265174506161884	25.213464941491537	29.118568594338413	22.402791958008166
40	23.234246471831767	24.66739183535039	31.256029648738366	20.842332044079477
41	21.276203162040055	25.631520587351485	30.894294656303433	22.197981594305023
42	22.438288906500688	27.135571984121263	29.129112242405498	21.297026866972548
43	21.92885497739266	26.052651463897668	29.74653070046726	22.271962858242407
44	22.29041424235981	26.08964209586636	30.638084008273246	20.981859653500578
45	22.48274795585023	25.315474736540594	30.548550863436912	21.65322644417226
46	22.520968680093418	25.678703412451696	31.074415310782815	20.72591259667207
47	22.29305015437658	26.32827999711807	31.08056577215528	20.29810407635007
48	21.769645891579668	25.84959837487238	31.572954136888182	20.807801596659772
49	22.381704661873993	24.856474590686794	31.658533413699363	21.10328733373985
50	20.52039932309779	24.805513625029217	33.103803972495136	21.570283079377855
51	20.84936114279087	25.44885188458923	32.52566060348327	21.176126369136632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	769.0
1	1846.5
2	2924.0
3	30545.0
4	58166.0
5	39276.5
6	20387.0
7	19617.5
8	18848.0
9	18633.5
10	18419.0
11	17807.5
12	17196.0
13	16273.0
14	15350.0
15	14442.5
16	13535.0
17	12394.5
18	11254.0
19	10330.0
20	9406.0
21	8944.5
22	8483.0
23	8267.5
24	8052.0
25	8560.0
26	9668.5
27	10269.0
28	11388.5
29	12508.0
30	14000.0
31	15492.0
32	17500.0
33	19508.0
34	21619.0
35	23730.0
36	26637.5
37	29545.0
38	33088.5
39	36632.0
40	40734.0
41	44836.0
42	48349.5
43	51863.0
44	56979.0
45	62095.0
46	67458.0
47	72821.0
48	74292.5
49	75764.0
50	74579.5
51	73395.0
52	70287.0
53	67179.0
54	64855.0
55	62531.0
56	61265.0
57	59999.0
58	57235.0
59	54471.0
60	50242.5
61	46014.0
62	40493.5
63	34973.0
64	29536.0
65	24099.0
66	20343.5
67	16588.0
68	14362.0
69	12136.0
70	10351.5
71	8567.0
72	7079.5
73	5592.0
74	4312.0
75	2241.5
76	1451.0
77	1031.0
78	611.0
79	466.0
80	321.0
81	236.0
82	151.0
83	106.5
84	62.0
85	43.5
86	25.0
87	14.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1138126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.16862561716146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80815972582994	31.30609540170528
2	11.45910803519762	10.58102537567291
3	6.696936296007393	9.275650338970358
4	4.413576027392737	8.15074956966296
5	2.8105005289275016	6.487847335844403
6	1.9492375281220904	5.399617060487401
7	1.368377878711619	4.422328818952962
8	0.9505624869143603	3.5108930867253627
9	0.6490298898879304	2.6968336200525043
>10	1.8392068918792588	12.035886877591265
>50	0.03342359028025973	1.0369518789692365
>100	0.02111336272633625	1.9409535324619895
>500	3.838790614942871E-4	0.09145155034956873
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.838790614942871E-4	3.063715552553915
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22082	1.9402069718115569	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12491	1.097505900049731	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.786373389238098E-5	0.0	0.0	0.004744641630188573	0.0
2	8.786373389238098E-5	0.0	0.0	0.019505748924108578	0.0
3	8.786373389238098E-5	0.0	0.0	0.0411202274616343	0.0
4	1.7572746778476197E-4	0.0	0.0	0.06510702681425432	0.0
5	1.7572746778476197E-4	0.0	0.0	0.12494222959496576	0.0
6	1.7572746778476197E-4	0.0	0.0	0.2846784978113144	0.0
7	1.7572746778476197E-4	0.0	0.0	0.3878305214009697	0.0
8	1.7572746778476197E-4	0.0	0.0	0.5597798486283593	0.0
9	1.7572746778476197E-4	0.0	0.0	0.9060508238982327	0.0
10	1.7572746778476197E-4	0.0	0.0	1.3494990888530796	0.0
11	1.7572746778476197E-4	0.0	0.0	1.5896306735809567	0.0
12	1.7572746778476197E-4	0.0	0.0	1.778801292651253	0.0
13	1.7572746778476197E-4	0.0	0.0	1.8990867443499226	0.0
14	1.7572746778476197E-4	0.0	0.0	1.9735951906906617	0.0
15	1.7572746778476197E-4	0.0	0.0	2.028334296905615	0.0
16	1.7572746778476197E-4	0.0	0.0	2.0905418205014206	0.0
17	1.7572746778476197E-4	0.0	0.0	2.1525736166294416	0.0
18	1.7572746778476197E-4	0.0	0.0	2.2563406863563436	0.0
19	1.7572746778476197E-4	0.0	0.0	2.316615207806517	0.0
20	1.7572746778476197E-4	0.0	0.0	2.381722234620771	0.0
21	1.7572746778476197E-4	0.0	0.0	2.4505195382585057	0.0
22	1.7572746778476197E-4	0.0	0.0	2.5231828461875048	0.0
23	1.7572746778476197E-4	0.0	0.0	2.5996242946738763	0.0
24	1.7572746778476197E-4	0.0	0.0	2.6723754663367676	0.0
25	1.7572746778476197E-4	0.0	0.0	2.739327631562762	0.0
26	1.7572746778476197E-4	0.0	0.0	2.807861343998819	0.0
27	1.7572746778476197E-4	0.0	0.0	2.882721245275128	0.0
28	1.7572746778476197E-4	0.0	0.0	2.9594262849631763	0.0
29	1.7572746778476197E-4	0.0	0.0	3.0396458740069203	0.0
30	1.7572746778476197E-4	0.0	0.0	3.1453459458794546	0.0
31	1.7572746778476197E-4	0.0	0.0	3.233561134707405	0.0
32	1.7572746778476197E-4	0.0	0.0	3.353231540268828	0.0
33	1.7572746778476197E-4	0.0	0.0	3.4441705048474422	0.0
34	1.7572746778476197E-4	0.0	0.0	3.538975473717321	0.0
35	1.7572746778476197E-4	0.0	0.0	3.6388765391529585	0.0
36	1.7572746778476197E-4	0.0	0.0	3.7420285627426138	0.0
37	1.7572746778476197E-4	0.0	0.0	3.849837364228565	0.0
38	1.7572746778476197E-4	0.0	0.0	3.9980634833050117	0.0
39	1.7572746778476197E-4	0.0	0.0	4.181786550873981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18655	0.0	44.191906	1
GTTTTTA	12715	0.0	43.035786	2
TCACGAC	180	0.0	41.250004	25
TAGCCGT	105	0.0	40.714287	44
GTAGCCG	105	0.0	40.714287	43
CACGACC	490	0.0	40.408165	27
TTATTTT	14010	0.0	40.230194	6
TATTTTT	14000	0.0	40.194645	7
CACGACG	185	0.0	40.135136	26
CGGTCTA	185	0.0	40.135136	31
AGACTTA	890	0.0	39.69101	8
TTTATTT	14230	0.0	39.560787	5
TTTGCGA	195	0.0	39.23077	2
TTTTATT	14395	0.0	39.185482	4
CGCCGTT	75	0.0	39.000004	26
ACACGAC	495	0.0	38.636364	26
ACGACCA	510	0.0	38.382355	28
CGACGGT	195	0.0	38.076923	28
TTTTTAT	14845	0.0	37.861233	3
TAGAGGC	500	0.0	37.35	5
>>END_MODULE