FastQCFastQC Report
Thu 26 May 2016
SRR1546894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546894_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2836129
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362041.2765286769395892No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201770.711427442122696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT294100.043.959541
GTTTTTA203300.042.3991622
AGCGATA3900.040.3846178
CGTACGA950.040.263162
TAATGCG950.040.263161
CGTAAGA3250.039.461542
AGACTTA19550.039.1304368
TATACGA1900.039.078952
TATTTTT231700.038.7947777
GAGCGAT22800.038.782897
TGTACGA1750.038.5714262
TATGCGA2050.038.4146352
TTATTTT236500.038.00746
TCACGAC4300.037.6744225
CGGTCTA4300.037.6744231
GCGTAAG2150.037.674421
TTTATTT239250.037.561135
TTTTATT240400.037.503124
TCGTAAG900.037.51
GCGCGAC10600.037.1462259