##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546894_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2836129
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720391068248308	31.0	31.0	34.0	30.0	34.0
2	32.06795142251992	33.0	31.0	34.0	30.0	34.0
3	31.99087100763047	33.0	31.0	34.0	30.0	34.0
4	35.8064767152693	37.0	35.0	37.0	35.0	37.0
5	35.83382667008447	37.0	35.0	37.0	35.0	37.0
6	35.88760772165159	37.0	35.0	37.0	35.0	37.0
7	36.09554925040434	37.0	35.0	37.0	35.0	37.0
8	13.280738287997478	0.0	0.0	35.0	0.0	37.0
9	24.901417742281822	18.0	17.0	38.0	17.0	39.0
10	32.67169617460983	32.0	32.0	38.0	27.0	39.0
11	35.6021764172222	37.0	35.0	37.0	32.0	39.0
12	36.75761398723401	38.0	35.0	39.0	33.0	39.0
13	37.061767994333124	39.0	37.0	39.0	33.0	39.0
14	38.29441608615123	40.0	38.0	41.0	34.0	41.0
15	38.51889423929588	40.0	38.0	41.0	34.0	41.0
16	38.56148468564018	40.0	38.0	41.0	34.0	41.0
17	38.50764016728435	40.0	38.0	41.0	34.0	41.0
18	38.416281487901294	40.0	38.0	41.0	34.0	41.0
19	38.35522291122865	40.0	37.0	41.0	34.0	41.0
20	38.29071103606359	40.0	37.0	41.0	34.0	41.0
21	38.2325521864485	40.0	37.0	41.0	34.0	41.0
22	38.174661660312346	40.0	37.0	41.0	34.0	41.0
23	37.99249328926858	40.0	36.0	41.0	34.0	41.0
24	37.72889808608847	40.0	35.0	41.0	33.0	41.0
25	37.6097518836414	39.0	35.0	41.0	33.0	41.0
26	37.53443514029157	39.0	35.0	41.0	33.0	41.0
27	37.480308547319254	39.0	35.0	41.0	33.0	41.0
28	37.34126162808532	39.0	35.0	41.0	33.0	41.0
29	37.26979590843717	39.0	35.0	41.0	33.0	41.0
30	37.10676347937629	39.0	35.0	41.0	32.0	41.0
31	36.96217026799557	39.0	35.0	41.0	32.0	41.0
32	36.67272680473984	39.0	35.0	41.0	31.0	41.0
33	36.33576223084352	39.0	35.0	41.0	30.0	41.0
34	35.976171041585204	39.0	35.0	41.0	27.0	41.0
35	35.70228716676851	39.0	35.0	41.0	25.0	41.0
36	35.52904539955693	39.0	35.0	41.0	24.0	41.0
37	35.465449914302205	39.0	35.0	41.0	23.0	41.0
38	35.340821239090324	39.0	35.0	41.0	23.0	41.0
39	35.232686877077874	39.0	35.0	41.0	23.0	41.0
40	35.11179428016145	39.0	35.0	41.0	23.0	41.0
41	35.011516753998144	39.0	35.0	40.0	22.0	41.0
42	34.934274851390754	39.0	35.0	40.0	22.0	41.0
43	34.82081280505929	38.0	34.0	40.0	21.0	41.0
44	34.622618717272736	38.0	34.0	40.0	20.0	41.0
45	34.53988164854278	38.0	34.0	40.0	20.0	41.0
46	34.50255013082973	38.0	34.0	40.0	20.0	41.0
47	34.46997650671038	38.0	34.0	40.0	20.0	41.0
48	34.377304769987546	38.0	34.0	40.0	20.0	41.0
49	34.254097045656245	38.0	34.0	40.0	20.0	41.0
50	34.159724398995955	37.0	34.0	40.0	20.0	41.0
51	33.82324217269384	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	5.0
12	9.0
13	7.0
14	23.0
15	72.0
16	170.0
17	384.0
18	1027.0
19	2115.0
20	3987.0
21	6796.0
22	11358.0
23	18388.0
24	29326.0
25	45919.0
26	60087.0
27	63437.0
28	59534.0
29	57886.0
30	62172.0
31	73208.0
32	93404.0
33	125877.0
34	186112.0
35	273349.0
36	303775.0
37	493821.0
38	599516.0
39	264293.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.216049058417305	21.27628891351557	24.785332402016973	20.722329626050154
2	39.252234295407575	22.740820322347822	25.551447060412276	12.455498321832328
3	29.37733086188957	22.579896753638497	35.377551585276976	12.665220799194959
4	25.91553487165076	24.743232765505375	34.73925198748012	14.601980375363743
5	21.876261622796424	29.64177581485186	33.708234004870725	14.77372855748099
6	20.475620114599867	37.69049292186639	32.03905746177272	9.794829501761026
7	78.74194015857529	4.475854236531554	14.601416226130759	2.1807893787623907
8	29.533423902791444	63.73786241740061	5.44968159064697	1.2790320891609654
9	73.14635547254727	6.144325593088325	16.542900552125804	4.166418382238608
10	38.27992309235581	26.30031990787443	22.092824409609012	13.326932590160744
11	32.004327024617005	23.000787340773286	28.968781039226354	16.026104595383355
12	28.94804855491411	20.59359782294811	33.91834433483103	16.54000928730675
13	23.264245032577854	24.05895500522014	34.347802938441795	18.328997023760202
14	18.874247257441393	28.115399546353498	35.23746627886108	17.772886917344028
15	18.939688568467794	22.620303942451137	40.77593085504926	17.664076634031808
16	21.90369337925038	20.53876956936726	39.175651037029695	18.38188601435266
17	21.28164833122894	20.749232492598185	34.27368783295823	23.695431343214644
18	23.273306679632697	22.953081471258887	34.71305430747332	19.060557541635095
19	25.568865167980725	24.522086266174774	31.23359339437663	18.67545517146787
20	27.15609903498748	23.268969782404113	30.46525739837645	19.10967378423196
21	24.969245051970486	24.711252555860472	31.840688487723934	18.478813904445108
22	22.626333287378678	22.486635833560463	32.80802812566001	22.07900275340085
23	22.5768644515112	24.544616976167163	32.11997761737918	20.758540954942458
24	22.396019363012048	23.09337833363715	34.899646666283516	19.610955637067285
25	22.435756624610516	22.980583746366968	33.41470010708258	21.16895952193994
26	19.947364876562386	25.239437275243827	32.81010842595665	22.00308942223714
27	20.47297566507024	24.149500957114434	34.040799977716105	21.33672340009922
28	19.36787783630434	25.25061448192237	35.615622561597164	19.765885120176126
29	21.22361147888548	24.67081010772077	34.623848210007374	19.481730203386377
30	22.033306665528965	23.95155509499039	34.73565553611983	19.279482703360813
31	25.924208666107923	23.23036081927162	30.7145761000293	20.130854414591155
32	25.79568841896825	24.537847185371326	30.669973051296328	18.996491344364095
33	24.2528460447321	24.896787134858815	30.50541777189966	20.34494904850943
34	21.233554609116865	26.258890198577006	31.826267422955727	20.681287769350405
35	21.73120475126484	26.287344475515745	30.875182334795063	21.106268438424344
36	24.17696797289545	28.21874463397116	28.217157964253385	19.387129428879998
37	22.686309402710524	29.287490096536512	27.8895635565237	20.136636944229263
38	22.43208965459611	30.069365674128363	26.596956626444	20.901588044831527
39	22.72082828390387	27.133250991051533	27.79492752269026	22.350993202354335
40	23.254160865038227	25.363091735248993	29.197049922623407	22.18569747708937
41	20.488948140229166	25.76409606192102	29.677352475857056	24.06960332199276
42	21.92678823847575	26.899446393305805	27.444661367659933	23.729104000558507
43	21.72514014701024	25.82107513445263	28.649507832683206	23.804276885853923
44	21.588439735992264	26.905510997560405	29.466466440701396	22.03958282574594
45	21.845762304888105	27.630760095891265	28.30960791980901	22.213869679411623
46	22.066979322872832	27.651563098857636	28.9946613852896	21.28679619297994
47	21.924743197506178	26.661163861023248	29.714692103215334	21.699400838255244
48	21.770624678919752	25.6613503828634	30.85416777586633	21.713857162350514
49	21.746189965266037	25.32603418250721	31.098585431057614	21.829190421169137
50	20.28796997597782	26.540682740453626	31.194349763357028	21.976997520211526
51	20.805647415896807	26.78344320727301	30.20356267292496	22.207346703905216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1784.0
1	3985.0
2	6186.0
3	50932.0
4	95678.0
5	64689.5
6	33701.0
7	32250.0
8	30799.0
9	30505.5
10	30212.0
11	29359.5
12	28507.0
13	27135.5
14	25764.0
15	24001.0
16	22238.0
17	20846.5
18	19455.0
19	18649.0
20	17843.0
21	17860.0
22	17877.0
23	18217.5
24	18558.0
25	20449.0
26	26599.5
27	30859.0
28	34799.0
29	38739.0
30	43017.5
31	47296.0
32	52470.5
33	57645.0
34	62702.5
35	67760.0
36	73858.0
37	79956.0
38	94191.0
39	108426.0
40	123950.0
41	139474.0
42	154135.0
43	168796.0
44	177980.5
45	187165.0
46	190357.0
47	193549.0
48	188954.5
49	184360.0
50	178211.0
51	172062.0
52	166528.0
53	160994.0
54	158259.0
55	155524.0
56	153600.5
57	151677.0
58	147757.0
59	143837.0
60	130911.0
61	117985.0
62	102833.0
63	87681.0
64	73637.0
65	59593.0
66	49843.5
67	40094.0
68	33816.0
69	27538.0
70	23657.0
71	19776.0
72	15950.5
73	12125.0
74	9271.5
75	4775.5
76	3133.0
77	2341.5
78	1550.0
79	1130.5
80	711.0
81	493.5
82	276.0
83	190.5
84	105.0
85	72.0
86	39.0
87	27.0
88	15.0
89	16.5
90	18.0
91	10.0
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2836129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.24994143731997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.83094925196669	19.846391019405146
2	6.394060170579816	3.4847553039000023
3	2.996963698198831	2.450012557970761
4	2.0124848801727504	2.1936038051279736
5	1.6236331021494428	2.2121953474633234
6	1.3175390424274964	2.1541717048519144
7	1.1606968543864795	2.2140244915959153
8	1.0200447483150152	2.223692772402396
9	0.8869087165767067	2.175138952826748
>10	9.428312826880928	50.697133652520506
>50	0.2783391908220161	4.775616878369032
>100	0.04419069560876359	2.260295207331784
>500	0.0041790733618032525	0.7970141812193247
>1k	0.0014365564681196557	0.5095040200266027
>5k	0.0	0.0
>10k+	2.611920851126647E-4	2.0064501049884673
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36204	1.2765286769395892	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20177	0.711427442122696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006875568777019663	0.0
2	0.0	0.0	0.0	0.025880346063243246	0.0
3	0.0	0.0	0.0	0.049504095194541575	0.0
4	0.0	0.0	0.0	0.0762659244343258	0.0
5	0.0	0.0	0.0	0.14614991067049488	0.0
6	0.0	0.0	0.0	0.2875750715147301	0.0
7	0.0	0.0	0.0	0.38048339832214967	0.0
8	0.0	0.0	0.0	0.5630209345202563	0.0
9	0.0	0.0	0.0	0.88680733492729	0.0
10	0.0	0.0	0.0	1.313797785643742	0.0
11	0.0	0.0	0.0	1.5515514280203757	0.0
12	0.0	0.0	0.0	1.756302340267315	0.0
13	0.0	0.0	0.0	1.8605641703885825	0.0
14	0.0	0.0	0.0	1.9137704949245962	0.0
15	0.0	0.0	0.0	1.9643676292580485	0.0
16	0.0	0.0	0.0	2.036472953099101	0.0
17	0.0	0.0	0.0	2.118979778423337	0.0
18	0.0	0.0	0.0	2.263930871973736	0.0
19	0.0	0.0	0.0	2.3270450674140704	0.0
20	0.0	0.0	0.0	2.396400163744315	0.0
21	0.0	0.0	0.0	2.4789422483956125	0.0
22	0.0	0.0	0.0	2.5670200473955873	0.0
23	0.0	0.0	0.0	2.6701535790508824	0.0
24	0.0	0.0	0.0	2.752871960337488	0.0
25	0.0	0.0	0.0	2.8243778756184925	0.0
26	0.0	0.0	0.0	2.894826011087648	0.0
27	0.0	0.0	0.0	2.9706688235972343	0.0
28	0.0	0.0	0.0	3.049191344963505	0.0
29	0.0	0.0	0.0	3.1383621831023905	0.0
30	0.0	0.0	0.0	3.2488296547865065	0.0
31	0.0	0.0	0.0	3.341526425631556	0.0
32	0.0	0.0	0.0	3.4429675095878927	0.0
33	0.0	0.0	0.0	3.5468767464385436	0.0
34	0.0	0.0	0.0	3.647330569237154	0.0
35	0.0	0.0	0.0	3.7639684231570567	0.0
36	0.0	0.0	0.0	3.874435894841173	0.0
37	0.0	0.0	0.0	3.9889229298103155	0.0
38	0.0	0.0	0.0	4.148682940726603	0.0
39	0.0	0.0	0.0	4.322581941794608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29410	0.0	43.95954	1
GTTTTTA	20330	0.0	42.399162	2
AGCGATA	390	0.0	40.384617	8
CGTACGA	95	0.0	40.26316	2
TAATGCG	95	0.0	40.26316	1
CGTAAGA	325	0.0	39.46154	2
AGACTTA	1955	0.0	39.130436	8
TATACGA	190	0.0	39.07895	2
TATTTTT	23170	0.0	38.794777	7
GAGCGAT	2280	0.0	38.78289	7
TGTACGA	175	0.0	38.571426	2
TATGCGA	205	0.0	38.414635	2
TTATTTT	23650	0.0	38.0074	6
TCACGAC	430	0.0	37.67442	25
CGGTCTA	430	0.0	37.67442	31
GCGTAAG	215	0.0	37.67442	1
TTTATTT	23925	0.0	37.56113	5
TTTTATT	24040	0.0	37.50312	4
TCGTAAG	90	0.0	37.5	1
GCGCGAC	1060	0.0	37.146225	9
>>END_MODULE