FastQCFastQC Report
Thu 26 May 2016
SRR1546893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546893_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1511371
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197671.3078853570698392No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109920.7272866820919549No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG21850.14457072419677233No Hit
GAATCTGACTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC19260.12743396558488948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT170400.044.1417241
GTTTTTA117900.042.6145022
TAACGCC1400.041.78571312
TCACGAC2600.041.53846425
CGGTCTA2750.040.90909231
TATTTTT131600.039.5630727
TTATTTT132100.039.3792576
GAGCGAT12400.039.0120967
TTTTATT134400.038.72214
TTTATTT134800.038.5738145
TATGCGA1050.038.571432
AGACTTA12400.038.286298
GCGATAA1000.038.259
CGCATCG950.037.89473721
CGACGGT3000.037.528
TTTTTAT139900.037.2158663
AGCGATA2000.037.1258
AGCGTAA250.002107318336.032
CCGTAAG250.002107318336.01
TTACGAG2500.036.03
Produced by FastQC (version 0.11.5)