FastQCFastQC Report
Thu 26 May 2016
SRR1546892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546892_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4249277
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214730.5053330248887047No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118570.2790357041915601No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67950.15990955637864981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT201850.043.2276461
CGACGGT9300.040.6451628
ATACGCG501.0822987E-940.51
TATAGCG2350.040.2127651
TCACGAC9550.040.05235725
GTTTTTA146500.039.9931762
CCAGTCG4650.039.6774228
AGGGGAA290700.039.4969068
GAGGGGA288550.039.2453657
CACGACG9700.038.73711426
CGTCGTC356.2522686E-638.5714317
CGCATCG4800.038.43750421
GAGCGAT33400.038.3308377
CGGTCTA9850.038.1472131
TATCACG659.094947E-1238.0769271
AGACTTA38400.037.9687548
AGCGATC3750.037.88
TTAGTCG601.5643309E-1037.5000041
TTTGCGA6000.037.52
GACGGTC10050.037.38805829
Produced by FastQC (version 0.11.5)