##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546892_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4249277
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680212657353238	31.0	31.0	33.0	30.0	34.0
2	32.0345743522957	31.0	31.0	34.0	30.0	34.0
3	32.054425964699405	33.0	31.0	34.0	30.0	34.0
4	35.81443054900869	37.0	35.0	37.0	35.0	37.0
5	35.80035121268865	37.0	35.0	37.0	35.0	37.0
6	35.84916116318141	37.0	35.0	37.0	35.0	37.0
7	36.09349119862038	37.0	35.0	37.0	35.0	37.0
8	13.3515941653133	0.0	0.0	36.0	0.0	37.0
9	25.000428072822743	18.0	17.0	39.0	17.0	39.0
10	32.4558241319641	32.0	32.0	37.0	27.0	39.0
11	35.46411613081472	37.0	34.0	37.0	32.0	39.0
12	36.60387755375797	37.0	35.0	39.0	33.0	39.0
13	36.873235846945256	39.0	35.0	39.0	33.0	39.0
14	38.0429736635197	40.0	37.0	41.0	33.0	41.0
15	38.313876219413324	40.0	37.0	41.0	33.0	41.0
16	38.39266421087635	40.0	37.0	41.0	34.0	41.0
17	38.36742321105449	40.0	37.0	41.0	34.0	41.0
18	38.31324787722711	40.0	37.0	41.0	34.0	41.0
19	38.298770355521654	40.0	37.0	41.0	34.0	41.0
20	38.26663547704704	40.0	37.0	41.0	34.0	41.0
21	38.208691972775604	40.0	37.0	41.0	34.0	41.0
22	38.1552125220361	40.0	37.0	41.0	34.0	41.0
23	38.03162279135957	40.0	37.0	41.0	34.0	41.0
24	37.93750160321391	40.0	36.0	41.0	34.0	41.0
25	37.83003885131518	40.0	36.0	41.0	33.0	41.0
26	37.76687492954684	40.0	36.0	41.0	33.0	41.0
27	37.706813182572	40.0	36.0	41.0	33.0	41.0
28	37.63249230398489	40.0	36.0	41.0	33.0	41.0
29	37.620339413034266	40.0	36.0	41.0	33.0	41.0
30	37.52171251721175	40.0	36.0	41.0	33.0	41.0
31	37.45911339740855	40.0	36.0	41.0	33.0	41.0
32	37.30867133397046	40.0	35.0	41.0	33.0	41.0
33	37.16218782630551	40.0	35.0	41.0	32.0	41.0
34	36.97726483823013	40.0	35.0	41.0	31.0	41.0
35	36.850785674833624	40.0	35.0	41.0	31.0	41.0
36	36.73928082353775	40.0	35.0	41.0	31.0	41.0
37	36.682542230125264	40.0	35.0	41.0	31.0	41.0
38	36.58571822924229	40.0	35.0	41.0	30.0	41.0
39	36.51708514177824	40.0	35.0	41.0	30.0	41.0
40	36.41946076944384	39.0	35.0	41.0	30.0	41.0
41	36.36412006089507	39.0	35.0	41.0	30.0	41.0
42	36.31361946985334	39.0	35.0	41.0	30.0	41.0
43	36.2170849299775	39.0	35.0	41.0	30.0	41.0
44	35.99235022805056	39.0	35.0	41.0	29.0	41.0
45	35.90737765506932	39.0	35.0	40.0	29.0	41.0
46	35.89622752294096	39.0	35.0	40.0	29.0	41.0
47	35.862892910958735	39.0	35.0	40.0	29.0	41.0
48	35.792054742489135	38.0	35.0	40.0	28.0	41.0
49	35.69259711710957	38.0	35.0	40.0	28.0	41.0
50	35.59282202595877	38.0	35.0	40.0	28.0	41.0
51	35.23644563533985	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	8.0
13	12.0
14	19.0
15	58.0
16	144.0
17	398.0
18	1040.0
19	2162.0
20	4322.0
21	7366.0
22	12256.0
23	18765.0
24	29015.0
25	42364.0
26	53553.0
27	58398.0
28	60678.0
29	67197.0
30	82018.0
31	105253.0
32	139307.0
33	191698.0
34	289886.0
35	432056.0
36	450667.0
37	771367.0
38	999960.0
39	429218.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46849358137867	22.80199666908041	28.627928939440757	15.101580810100165
2	32.89397231576101	24.723829489110734	29.595411172300608	12.786787022827648
3	29.58893948311678	24.52158331876223	32.795979174810206	13.09349802331079
4	26.067069762691393	26.787615869711484	31.795103025761794	15.35021134183533
5	23.00381923795507	31.60295269054006	30.79159113420942	14.601636937295451
6	20.56382768174445	40.561912061746035	28.27339803924291	10.600862217266608
7	86.18096678564376	4.066644749212631	8.062924586935614	1.689463878207987
8	32.88670049045991	63.27544662303728	2.841448086345042	0.996404800157768
9	82.66410968265896	4.231755190353558	9.68501229738612	3.4191228296013647
10	41.12744356275197	23.13883044103738	20.04955195907445	15.6841740371362
11	37.05804540395931	22.640510373882428	22.768249751663635	17.533194470494628
12	32.99855481297171	22.900813479563702	26.662771102001585	17.437860605462998
13	24.259609340600765	30.356811288132075	27.584433775439916	17.79914559582724
14	19.318133414225525	34.162352795546155	29.404155106857	17.115358683371312
15	18.575136429091348	24.617293718437278	39.81326705696052	16.99430279551086
16	21.260534439152824	20.840863045642823	38.63332515154931	19.265277363655038
17	20.55886213113431	21.136183873162423	29.762498420319506	28.542455575383762
18	23.31100090674249	23.860435551742096	32.50270575441422	20.325857787101196
19	26.746055858443686	25.432585355108646	27.486040566430475	20.335318220017193
20	27.715962033070568	25.49765524817516	25.835053822097265	20.951328896657007
21	24.723711822034666	26.660182426328056	29.148182149575092	19.467923602062186
22	23.11910943908811	23.621853788303284	28.736559184068255	24.522477588540355
23	20.662385624660384	28.466395577412346	28.351105376279307	22.520113421647963
24	21.554725662742154	26.065351823380777	33.46253962732955	18.917382886547525
25	21.36739967763928	24.882515307898263	32.215904023202064	21.534180991260396
26	19.661674209518466	29.035292356793875	28.894256599416796	22.408776834270867
27	20.365982260040944	28.13175041307027	30.60861883092112	20.893648495967668
28	17.953807200613188	27.41939393454463	34.71482325110837	19.91197561373382
29	19.530098885057388	26.696000284283656	33.4574093428129	20.31649148784605
30	21.388085549612324	26.979201402968084	31.668846253139066	19.963866794280534
31	26.612386060028566	25.39441415563165	27.13155673306306	20.861643051276722
32	25.658011939442876	26.86435833672411	28.032274666960994	19.445355056872028
33	24.67928073411077	25.690511585853308	27.73928364754757	21.89092403248835
34	20.2658946451361	28.078517827856363	29.545167330818867	22.110420196188667
35	19.89898516853573	26.126915237580416	30.16870870032714	23.805390893556716
36	25.30277503678861	27.472979520986744	27.31775311423567	19.90649232798897
37	21.357798044231995	29.783466693275113	27.634677616921653	21.224057645571236
38	21.511730113146307	30.757655949470937	25.000676585687398	22.72993735169536
39	21.02094073885981	28.100945172555235	26.890715761763705	23.987398326821243
40	22.82002326513428	25.727223713587037	27.723139724710816	23.729613296567862
41	19.99260109425674	24.96987134517237	28.62143371684171	26.416093843729183
42	21.82827808118887	27.556970279885263	26.174405669482127	24.440345969443744
43	21.902761340340955	26.731935809315328	26.870100490036304	24.49520236030741
44	21.521590614120946	28.407326705225383	28.159049174718433	21.912033505935245
45	21.04878076905789	29.96796866855232	26.250724535020897	22.73252602736889
46	23.285231817083236	28.575967158648403	27.27487523171589	20.86392579255247
47	22.468128107440393	27.42090007311832	27.811978367143396	22.298993452297886
48	22.33963566037234	26.436191380321876	28.593593686643633	22.63057927266215
49	21.674322478859345	25.2600148213449	29.920219369083256	23.145443330712496
50	20.560744804351422	28.11772449760277	28.855826532372447	22.465704165673362
51	20.461292591657358	28.971446201318486	27.317188312270535	23.250072894753625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2582.0
1	4576.5
2	6571.0
3	31703.5
4	56836.0
5	39024.5
6	21213.0
7	21087.5
8	20962.0
9	21539.0
10	22116.0
11	21971.5
12	21827.0
13	20872.5
14	19918.0
15	18979.5
16	18041.0
17	17029.0
18	16017.0
19	16059.5
20	16102.0
21	16452.5
22	16803.0
23	18681.5
24	20560.0
25	23386.5
26	30578.5
27	34944.0
28	42160.5
29	49377.0
30	56770.5
31	64164.0
32	73020.0
33	81876.0
34	93949.5
35	106023.0
36	123835.0
37	141647.0
38	173943.0
39	206239.0
40	248871.5
41	291504.0
42	318469.5
43	345435.0
44	360183.0
45	374931.0
46	379116.5
47	383302.0
48	370170.0
49	357038.0
50	340540.0
51	324042.0
52	300150.5
53	276259.0
54	253733.0
55	231207.0
56	212971.0
57	194735.0
58	178236.0
59	161737.0
60	141305.0
61	120873.0
62	101754.5
63	82636.0
64	67315.5
65	51995.0
66	41905.5
67	31816.0
68	26297.0
69	20778.0
70	17337.0
71	13896.0
72	11150.0
73	8404.0
74	6439.0
75	3358.0
76	2242.0
77	1670.0
78	1098.0
79	765.5
80	433.0
81	336.5
82	240.0
83	179.5
84	119.0
85	72.5
86	26.0
87	18.5
88	11.0
89	7.0
90	3.0
91	3.5
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4249277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.4825286381496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60309492236219	15.895076141563253
2	6.840178097790547	2.802082875560771
3	2.571662478545163	1.5802245109336823
4	1.4480444159230017	1.1863844487382196
5	0.9598460080112006	0.9830036673651492
6	0.6590238853498435	0.8099085362941673
7	0.5807847923179744	0.8327158798878274
8	0.48545087499460876	0.7954609159593484
9	0.41628339498896627	0.767388290350288
>10	5.996232516027375	30.15931230716264
>50	1.9060992206162333	27.190108942383905
>100	0.5256121043388422	14.547950237246429
>500	0.004658962869160631	0.6589734990726268
>1k	0.002678903649736322	0.8341542391889171
>5k	1.1647407172766618E-4	0.1621071944884511
>10k+	2.3294814345533235E-4	0.7951483138042788
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21473	0.5053330248887047	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11857	0.2790357041915601	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6795	0.15990955637864981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3533415213929333E-5	0.0	0.0	0.009601633407283169	0.0
2	2.3533415213929333E-5	0.0	0.0	0.039324336822475915	0.0
3	2.3533415213929333E-5	0.0	0.0	0.06471689183830567	0.0
4	2.3533415213929333E-5	0.0	0.0	0.103900028169498	0.0
5	2.3533415213929333E-5	0.0	0.0	0.20379937575262805	0.0
6	2.3533415213929333E-5	0.0	0.0	0.33881057883494065	0.0
7	2.3533415213929333E-5	0.0	0.0	0.43134396745611076	0.0
8	2.3533415213929333E-5	0.0	0.0	0.6583002237792452	0.0
9	2.3533415213929333E-5	0.0	0.0	0.9340883166712831	0.0
10	2.3533415213929333E-5	0.0	0.0	1.3975318624791935	0.0
11	2.3533415213929333E-5	0.0	0.0	1.6956531664092502	0.0
12	2.3533415213929333E-5	0.0	0.0	1.9863849779621334	0.0
13	2.3533415213929333E-5	0.0	0.0	2.1093235390397003	0.0
14	2.3533415213929333E-5	0.0	0.0	2.1584848434215984	0.0
15	2.3533415213929333E-5	0.0	0.0	2.2234370694120433	0.0
16	2.3533415213929333E-5	0.0	0.0	2.3703091137621763	0.0
17	2.3533415213929333E-5	0.0	0.0	2.5288066652279904	0.0
18	2.3533415213929333E-5	0.0	0.0	2.784379554451263	0.0
19	2.3533415213929333E-5	0.0	0.0	2.88599684134501	0.0
20	2.3533415213929333E-5	0.0	0.0	3.000792840758557	0.0
21	2.3533415213929333E-5	0.0	0.0	3.1521597674145507	0.0
22	2.3533415213929333E-5	0.0	0.0	3.3120693237932004	0.0
23	2.3533415213929333E-5	0.0	0.0	3.4938649563208046	0.0
24	2.3533415213929333E-5	0.0	0.0	3.631653102398361	0.0
25	2.3533415213929333E-5	0.0	0.0	3.7531090583174502	0.0
26	2.3533415213929333E-5	0.0	0.0	3.872094005639077	0.0
27	2.3533415213929333E-5	0.0	0.0	3.9938323625407333	0.0
28	4.7066830427858666E-5	0.0	0.0	4.130208503705454	0.0
29	4.7066830427858666E-5	0.0	0.0	4.276374545599169	0.0
30	4.7066830427858666E-5	0.0	0.0	4.4524515582297886	0.0
31	4.7066830427858666E-5	0.0	0.0	4.611207977262955	0.0
32	4.7066830427858666E-5	0.0	0.0	4.76233956976681	0.0
33	4.7066830427858666E-5	0.0	0.0	4.928108946533728	0.0
34	7.0600245641788E-5	0.0	0.0	5.0972200682610245	0.0
35	7.0600245641788E-5	0.0	0.0	5.2793451686016235	0.0
36	7.0600245641788E-5	0.0	0.0	5.441019731121318	0.0
37	7.0600245641788E-5	0.0	0.0	5.605424169805828	0.0
38	7.0600245641788E-5	0.0	0.0	5.797527438197133	0.0
39	7.0600245641788E-5	0.0	0.0	6.0382272090052025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	20185	0.0	43.227646	1
CGACGGT	930	0.0	40.64516	28
ATACGCG	50	1.0822987E-9	40.5	1
TATAGCG	235	0.0	40.212765	1
TCACGAC	955	0.0	40.052357	25
GTTTTTA	14650	0.0	39.993176	2
CCAGTCG	465	0.0	39.67742	28
AGGGGAA	29070	0.0	39.496906	8
GAGGGGA	28855	0.0	39.245365	7
CACGACG	970	0.0	38.737114	26
CGTCGTC	35	6.2522686E-6	38.57143	17
CGCATCG	480	0.0	38.437504	21
GAGCGAT	3340	0.0	38.330837	7
CGGTCTA	985	0.0	38.14721	31
TATCACG	65	9.094947E-12	38.076927	1
AGACTTA	3840	0.0	37.968754	8
AGCGATC	375	0.0	37.8	8
TTAGTCG	60	1.5643309E-10	37.500004	1
TTTGCGA	600	0.0	37.5	2
GACGGTC	1005	0.0	37.388058	29
>>END_MODULE