FastQCFastQC Report
Thu 26 May 2016
SRR1546891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546891_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1265650
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228201.8030261130644334No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126851.0022518073717064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT199900.044.1670841
GTTTTTA132850.043.0692522
CGTTAGA750.042.0000042
AGCGATA1800.041.258
ATTGCGA1100.040.9090922
TTATTTT149350.040.0284586
TATTTTT150200.039.7869537
TAAGCGA850.039.7058832
TATGCGA800.039.3752
TTTATTT152150.039.2326665
CACGACG1950.039.2307726
TTTTATT153650.038.9521644
TCTAGCG356.2487907E-638.5714261
TATTACG700.038.5714261
TCACGAC2050.038.41463525
CGGTCTA2050.038.41463531
ACCCGCT4350.038.2758634
CGACGGT2000.038.25000428
GCGCGAC4650.038.2258039
TTTTTAT157800.037.7423973
Produced by FastQC (version 0.11.5)