##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546891_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1265650
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754506380120887	31.0	31.0	34.0	30.0	34.0
2	32.10374669142338	33.0	31.0	34.0	30.0	34.0
3	31.938504325840476	33.0	31.0	34.0	30.0	34.0
4	35.811805791490535	37.0	35.0	37.0	35.0	37.0
5	35.872833721803026	37.0	35.0	37.0	35.0	37.0
6	35.93063959230435	37.0	35.0	37.0	35.0	37.0
7	36.13097459803263	37.0	35.0	37.0	35.0	37.0
8	13.128262157784537	0.0	0.0	36.0	0.0	37.0
9	24.8432591948801	18.0	17.0	39.0	17.0	39.0
10	32.73099593094457	32.0	32.0	38.0	27.0	39.0
11	35.69040809070438	37.0	35.0	37.0	32.0	39.0
12	36.881493303835974	39.0	37.0	39.0	33.0	39.0
13	37.193483190455495	39.0	37.0	39.0	34.0	39.0
14	38.43390036740015	40.0	38.0	41.0	34.0	41.0
15	38.642203610792876	40.0	38.0	41.0	35.0	41.0
16	38.67298305218662	40.0	38.0	41.0	34.0	41.0
17	38.636211432860584	40.0	38.0	41.0	34.0	41.0
18	38.497031564808594	40.0	38.0	41.0	34.0	41.0
19	38.38493501362936	40.0	37.0	41.0	34.0	41.0
20	38.29790305376684	40.0	37.0	41.0	34.0	41.0
21	38.25899103227591	40.0	37.0	41.0	34.0	41.0
22	38.204834669932445	40.0	37.0	41.0	34.0	41.0
23	37.955878007348005	39.0	36.0	41.0	34.0	41.0
24	37.52586655078418	39.0	35.0	41.0	33.0	41.0
25	37.41115316240667	39.0	35.0	41.0	33.0	41.0
26	37.33797969422826	39.0	35.0	41.0	33.0	41.0
27	37.28048433611188	39.0	35.0	41.0	33.0	41.0
28	37.10926006399873	39.0	35.0	41.0	33.0	41.0
29	36.9826839963655	39.0	35.0	41.0	33.0	41.0
30	36.74897088452574	39.0	35.0	41.0	32.0	41.0
31	36.49203571287481	39.0	35.0	41.0	31.0	41.0
32	36.03990755738158	39.0	35.0	41.0	30.0	41.0
33	35.522967645083554	39.0	35.0	41.0	24.0	41.0
34	34.975785564729584	39.0	35.0	41.0	21.0	41.0
35	34.53027614269348	39.0	34.0	41.0	18.0	41.0
36	34.32354126338245	39.0	34.0	41.0	16.0	41.0
37	34.228356180618654	38.0	34.0	41.0	15.0	41.0
38	34.09464701931814	38.0	34.0	40.0	15.0	41.0
39	33.95693517165093	38.0	34.0	40.0	15.0	41.0
40	33.8451167384348	38.0	33.0	40.0	14.0	41.0
41	33.72697981274444	38.0	33.0	40.0	12.0	41.0
42	33.64764429344606	38.0	33.0	40.0	12.0	41.0
43	33.5347513135543	38.0	33.0	40.0	11.0	41.0
44	33.33129696203532	38.0	33.0	40.0	10.0	41.0
45	33.255426065657964	37.0	33.0	40.0	10.0	41.0
46	33.21600600481966	37.0	33.0	40.0	10.0	41.0
47	33.191509501046895	37.0	33.0	40.0	11.0	41.0
48	33.10556868012484	37.0	33.0	40.0	11.0	41.0
49	33.00597084502034	37.0	32.0	40.0	10.0	41.0
50	32.92339983407735	36.0	32.0	40.0	10.0	41.0
51	32.58734879311026	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	4.0
14	12.0
15	30.0
16	66.0
17	165.0
18	491.0
19	1050.0
20	2097.0
21	3735.0
22	6564.0
23	10904.0
24	18211.0
25	28954.0
26	37802.0
27	39308.0
28	34123.0
29	29890.0
30	29491.0
31	33199.0
32	41134.0
33	55452.0
34	80400.0
35	119740.0
36	132378.0
37	212489.0
38	241624.0
39	106308.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.555840872279067	19.64697981274444	23.299016315727098	26.4981629992494
2	43.88480227551061	21.022083514399714	24.28491289060957	10.80820131948011
3	26.594793189270334	21.944692450519497	40.01611820013432	11.44439616007585
4	23.898234108955872	23.280132738118752	39.16043139888595	13.661201754039427
5	20.589736499032117	27.17749772843993	38.615256982578124	13.617508789949827
6	18.9358827479951	34.97483506498637	36.738592817919645	9.350689369098882
7	74.0133528226603	3.8532769723067197	20.15912772093391	1.9742424840990795
8	27.476395527989574	64.06320862797772	7.425670604037451	1.0347252399952593
9	69.46422786710386	4.971674633587485	21.810769169991705	3.7533283293169517
10	35.892861375577766	22.92885078813258	28.349148658791922	12.829139177497728
11	29.127641923122503	21.469679611266937	33.57033935132145	15.832339114289102
12	26.116619918618895	19.262671354639906	38.6587129143128	15.961995812428398
13	22.23900762454075	21.342472247461778	39.05819144313199	17.360328684865483
14	18.572986212618023	24.438114802670565	39.792991743372966	17.195907241338443
15	18.174850867143366	21.044917631256666	43.519061351874534	17.261170149725437
16	21.125350610358314	19.08300082961324	42.15027851301703	17.641370047011417
17	21.134673882985027	19.286848654841386	37.96484020068739	21.613637261486193
18	22.478489313791332	20.931221111681744	38.93430253229565	17.655987042231263
19	23.7131908505511	22.68020384782523	35.947457827993524	17.659147473630153
20	24.488523683482796	22.402480938648125	34.857108995377864	18.25188638249121
21	23.560067949275076	22.864377987595308	36.6817050527397	16.893849010389918
22	22.13716272271165	20.244380357918857	37.2581677398965	20.360289179473
23	20.900801959467465	22.904436455576185	36.727215264883654	19.46754632007269
24	21.158851183186506	22.11061509896101	38.708489708845256	18.022044009007228
25	21.682929719906767	21.469916643621854	37.07004306087781	19.777110575593568
26	19.482242326077507	22.848180776676017	36.74246434638328	20.927112550863193
27	19.01426144668747	21.844111721249952	38.655078418204084	20.48654841385849
28	18.743649508157866	22.713704420653418	39.5200094812942	19.02263658989452
29	20.1253901157508	23.225852328842887	38.165922648441516	18.4828349069648
30	21.14802670564532	21.846798087939003	38.71465255007308	18.290522656342592
31	24.63200726899222	21.11010152885869	35.36617548295342	18.89171571919567
32	24.545174416307827	22.39244656895666	34.57575159009205	18.486627424643466
33	24.024019278631535	22.660925216292025	33.59917828783629	19.715877217240152
34	21.561174100264687	23.98419784300557	35.02247856832458	19.432149488405166
35	22.01358985501521	24.922371903764866	33.48563978983131	19.578398451388615
36	24.448386204716943	26.52960929166831	30.533322798562	18.48868170505274
37	22.84502034527713	27.621775372338327	29.85620037135069	19.677003911033854
38	23.059613637261485	28.339825386165213	29.00161972109193	19.59894125548137
39	22.703037964682178	26.69268755185083	29.09177102674515	21.512503456721845
40	22.890372535851146	25.398727926361946	30.652787105439895	21.058112432347016
41	21.038833800813812	25.419428752024654	30.686524710622997	22.855212736538537
42	21.440050566902382	27.135543001619723	29.152135266463873	22.272271165014025
43	21.1909295618852	26.091731521352663	29.578872516098446	23.13846640066369
44	21.4402875992573	26.067080156441353	31.092719156164815	21.39991308813653
45	21.791095484533642	26.39039228854739	30.26444909730178	21.554063129617195
46	22.38170110220045	26.32505036937542	31.118476672065736	20.174771856358394
47	21.482795401572314	26.065657962311857	31.958203294749733	20.493343341366096
48	21.33014656500612	25.40173033619089	32.710623000039504	20.55750009876348
49	21.2836882234425	24.945917117686562	32.82558369217398	20.944810966696952
50	20.48330897167463	25.377632046774384	33.234148461264965	20.90491052028602
51	20.573144234187968	25.775293327539213	32.39884644253941	21.252715995733418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	964.0
1	2222.0
2	3480.0
3	33008.5
4	62537.0
5	42541.0
6	22545.0
7	21975.5
8	21406.0
9	21085.0
10	20764.0
11	20031.5
12	19299.0
13	18402.5
14	17506.0
15	16265.0
16	15024.0
17	13914.0
18	12804.0
19	11847.5
20	10891.0
21	10417.0
22	9943.0
23	9929.5
24	9916.0
25	10250.0
26	11419.0
27	12254.0
28	13168.5
29	14083.0
30	15999.5
31	17916.0
32	20675.5
33	23435.0
34	25745.0
35	28055.0
36	30452.0
37	32849.0
38	37957.5
39	43066.0
40	47746.0
41	52426.0
42	56397.0
43	60368.0
44	63574.5
45	66781.0
46	70821.5
47	74862.0
48	77723.0
49	80584.0
50	80956.5
51	81329.0
52	77875.5
53	74422.0
54	70770.0
55	67118.0
56	65546.5
57	63975.0
58	61055.0
59	58135.0
60	54663.0
61	51191.0
62	46505.5
63	41820.0
64	35942.5
65	30065.0
66	24869.0
67	19673.0
68	16436.0
69	13199.0
70	11150.0
71	9101.0
72	7373.0
73	5645.0
74	4363.0
75	2203.0
76	1325.0
77	1009.0
78	693.0
79	519.5
80	346.0
81	229.0
82	112.0
83	73.0
84	34.0
85	31.0
86	28.0
87	16.5
88	5.0
89	5.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1265650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.49048269838071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.08147055882753	27.280606178820747
2	8.676306144720302	6.852630353878626
3	5.128626627537992	6.07595823303731
4	3.640848525676157	5.751154628425567
5	2.7307197550134688	5.391872061974289
6	2.1736655996083063	5.15034622520383
7	1.7161810304546503	4.7440972103320975
8	1.3877457249268474	4.384219883198037
9	1.0654368967346954	3.7867155603046134
>10	4.333087080014148	24.380690196033168
>50	0.039073657598171566	1.046611111493262
>100	0.024601867274155476	1.8678444922399773
>500	0.0018298895020310202	0.43646984710822306
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.0664211156244887E-4	2.850784017950232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22820	1.8030261130644334	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12685	1.0022518073717064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.90107849721487E-5	0.0	0.0	0.005688776517994707	0.0
2	7.90107849721487E-5	0.0	0.0	0.027653774740252043	0.0
3	7.90107849721487E-5	0.0	0.0	0.05625567890016987	0.0
4	7.90107849721487E-5	0.0	0.0	0.07664046142298424	0.0
5	7.90107849721487E-5	0.0	0.0	0.13534547465729072	0.0
6	7.90107849721487E-5	0.0	0.0	0.2488839726622684	0.0
7	7.90107849721487E-5	0.0	0.0	0.31675423695334415	0.0
8	7.90107849721487E-5	0.0	0.0	0.43345316635720776	0.0
9	7.90107849721487E-5	0.0	0.0	0.7164697981274444	0.0
10	7.90107849721487E-5	0.0	0.0	1.1072571405996918	0.0
11	7.90107849721487E-5	0.0	0.0	1.3554300161972108	0.0
12	7.90107849721487E-5	0.0	0.0	1.5638604669537393	0.0
13	7.90107849721487E-5	0.0	0.0	1.6797692885078814	0.0
14	7.90107849721487E-5	0.0	0.0	1.7511160273377315	0.0
15	7.90107849721487E-5	0.0	0.0	1.8024730375696283	0.0
16	7.90107849721487E-5	0.0	0.0	1.864022439062932	0.0
17	7.90107849721487E-5	0.0	0.0	1.9172757081341603	0.0
18	7.90107849721487E-5	0.0	0.0	2.0146960060048196	0.0
19	7.90107849721487E-5	0.0	0.0	2.0692134476356023	0.0
20	7.90107849721487E-5	0.0	0.0	2.1284715363647138	0.0
21	7.90107849721487E-5	0.0	0.0	2.182593924070636	0.0
22	7.90107849721487E-5	0.0	0.0	2.244380357918856	0.0
23	7.90107849721487E-5	0.0	0.0	2.317307312448149	0.0
24	7.90107849721487E-5	0.0	0.0	2.3769604551021213	0.0
25	7.90107849721487E-5	0.0	0.0	2.429344605538656	0.0
26	7.90107849721487E-5	0.0	0.0	2.487259510923241	0.0
27	7.90107849721487E-5	0.0	0.0	2.549520009481294	0.0
28	7.90107849721487E-5	0.0	0.0	2.612570615889069	0.0
29	7.90107849721487E-5	0.0	0.0	2.679097696835618	0.0
30	7.90107849721487E-5	0.0	0.0	2.769328013273812	0.0
31	7.90107849721487E-5	0.0	0.0	2.834748943230751	0.0
32	7.90107849721487E-5	0.0	0.0	2.9162090625370363	0.0
33	7.90107849721487E-5	0.0	0.0	2.991664362185438	0.0
34	7.90107849721487E-5	0.0	0.0	3.068620866748311	0.0
35	7.90107849721487E-5	0.0	0.0	3.1537944929482875	0.0
36	7.90107849721487E-5	0.0	0.0	3.2347015367597676	0.0
37	7.90107849721487E-5	0.0	0.0	3.3216134002291313	0.0
38	7.90107849721487E-5	0.0	0.0	3.4402085884723266	0.0
39	7.90107849721487E-5	0.0	0.0	3.585114368111247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	19990	0.0	44.167084	1
GTTTTTA	13285	0.0	43.069252	2
CGTTAGA	75	0.0	42.000004	2
AGCGATA	180	0.0	41.25	8
ATTGCGA	110	0.0	40.909092	2
TTATTTT	14935	0.0	40.028458	6
TATTTTT	15020	0.0	39.786953	7
TAAGCGA	85	0.0	39.705883	2
TATGCGA	80	0.0	39.375	2
TTTATTT	15215	0.0	39.232666	5
CACGACG	195	0.0	39.23077	26
TTTTATT	15365	0.0	38.952164	4
TCTAGCG	35	6.2487907E-6	38.571426	1
TATTACG	70	0.0	38.571426	1
TCACGAC	205	0.0	38.414635	25
CGGTCTA	205	0.0	38.414635	31
ACCCGCT	435	0.0	38.27586	34
CGACGGT	200	0.0	38.250004	28
GCGCGAC	465	0.0	38.225803	9
TTTTTAT	15780	0.0	37.742397	3
>>END_MODULE