FastQCFastQC Report
Thu 26 May 2016
SRR1546890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546890_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2997565
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT549601.8334881812404402No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303501.0124884698079941No Hit
CGTTTCTATCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT36140.12056452487268833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT331900.044.064481
GTTTTTA224250.043.314382
TCACGAC11150.043.18385725
CACGACG11150.042.7802726
CGACGGT11450.041.85589228
CTATCTC23150.041.598276
TCGTTAG1250.041.41
GACGGTC11800.040.80508429
CGGTCTA11750.040.78723531
TATCTCT23700.040.632917
TTATTTT244850.040.3594066
AGCGATA3850.040.3246738
AGACTTA33600.040.2455378
CGCATCG5650.040.2212421
TCTCACG12050.040.1452323
TATTTTT246550.040.035497
CTCACGA12100.039.97933624
ATAACGC6550.039.8473311
ACGACGG12000.039.7527
ACGGTCT12000.039.7530
Produced by FastQC (version 0.11.5)