##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546890_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2997565
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58987745053068	31.0	31.0	33.0	30.0	34.0
2	31.925044494447995	31.0	31.0	34.0	30.0	34.0
3	31.8410029473923	31.0	31.0	34.0	30.0	34.0
4	35.684650708158124	37.0	35.0	37.0	33.0	37.0
5	35.707011190749824	37.0	35.0	37.0	33.0	37.0
6	35.7623954776627	37.0	35.0	37.0	35.0	37.0
7	36.01965328524986	37.0	35.0	37.0	35.0	37.0
8	13.313032077703069	0.0	0.0	35.0	0.0	37.0
9	24.898655075035904	18.0	17.0	38.0	17.0	39.0
10	32.485997801548926	32.0	32.0	37.0	27.0	39.0
11	35.41987579919034	37.0	34.0	37.0	32.0	39.0
12	36.58299586497707	37.0	35.0	39.0	33.0	39.0
13	36.87887768905762	39.0	37.0	39.0	33.0	39.0
14	38.09537474583537	40.0	37.0	41.0	33.0	41.0
15	38.33821184861713	40.0	37.0	41.0	33.0	41.0
16	38.39903054646021	40.0	38.0	41.0	34.0	41.0
17	38.37859495957552	40.0	37.0	41.0	34.0	41.0
18	38.28525853484412	40.0	37.0	41.0	33.0	41.0
19	38.233033478840326	40.0	37.0	41.0	34.0	41.0
20	38.151616061703415	40.0	37.0	41.0	34.0	41.0
21	38.08142509003141	40.0	36.0	41.0	33.0	41.0
22	38.02472840455503	40.0	36.0	41.0	33.0	41.0
23	37.82253028708302	39.0	36.0	41.0	33.0	41.0
24	37.542481981208084	39.0	35.0	41.0	33.0	41.0
25	37.42157284329114	39.0	35.0	41.0	33.0	41.0
26	37.337303778233334	39.0	35.0	41.0	33.0	41.0
27	37.27873890974841	39.0	35.0	41.0	33.0	41.0
28	37.13874761681565	39.0	35.0	41.0	33.0	41.0
29	37.05012201570275	39.0	35.0	41.0	32.0	41.0
30	36.87233104202912	39.0	35.0	41.0	32.0	41.0
31	36.72411740863001	39.0	35.0	41.0	31.0	41.0
32	36.42915833351404	39.0	35.0	41.0	30.0	41.0
33	36.11999105940989	39.0	35.0	41.0	30.0	41.0
34	35.773370719233775	39.0	35.0	41.0	27.0	41.0
35	35.48936553502593	39.0	35.0	41.0	25.0	41.0
36	35.336787359073114	39.0	35.0	41.0	24.0	41.0
37	35.26680922682244	39.0	35.0	41.0	24.0	41.0
38	35.156138399000525	38.0	35.0	40.0	23.0	41.0
39	35.05054902896184	38.0	35.0	40.0	23.0	41.0
40	34.91540733895679	38.0	35.0	40.0	23.0	41.0
41	34.80572698173351	38.0	34.0	40.0	22.0	41.0
42	34.70111640614966	38.0	34.0	40.0	21.0	41.0
43	34.55826278996452	38.0	34.0	40.0	21.0	41.0
44	34.3419555539246	38.0	34.0	40.0	20.0	41.0
45	34.23863002136734	38.0	34.0	40.0	20.0	41.0
46	34.19067409714218	38.0	34.0	40.0	20.0	41.0
47	34.131833338059394	37.0	33.0	40.0	20.0	41.0
48	34.040877178643335	37.0	33.0	40.0	20.0	41.0
49	33.930186668179005	37.0	33.0	40.0	20.0	41.0
50	33.79698588687818	37.0	33.0	40.0	20.0	41.0
51	33.46414706603527	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	12.0
14	32.0
15	74.0
16	162.0
17	488.0
18	1124.0
19	2448.0
20	4737.0
21	7825.0
22	12912.0
23	20502.0
24	32672.0
25	49511.0
26	63636.0
27	67211.0
28	64430.0
29	64013.0
30	72053.0
31	86684.0
32	110202.0
33	146101.0
34	213032.0
35	328151.0
36	325818.0
37	493844.0
38	583352.0
39	246483.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.6946254710073	21.506255911047802	25.112249442464137	21.686869175480766
2	38.76763306216879	22.631702732050847	26.57767221061095	12.022991995169413
3	27.865751034589742	22.691951634076325	36.892677890220895	12.549619441113036
4	24.378654007502757	24.86244668589338	36.01860176509934	14.740297541504521
5	21.07610677333102	29.199233377758283	35.19666796216262	14.527991886748076
6	18.63799450554033	37.97038596327352	32.85456695684664	10.537052574339507
7	77.56926038301087	4.229366168873735	15.663847155941573	2.537526292173814
8	29.268222707430862	63.44773174226413	5.730517937058913	1.5535276132460847
9	72.16360612697305	5.503500341110201	17.997674779362583	4.335218752554156
10	38.59612718990247	24.05128829566665	23.648394613628064	13.704189900802819
11	31.480384912420583	21.435531840010142	30.429231726417942	16.654851521151333
12	28.279987256323047	19.867992854199994	34.76708595143057	17.084933938046383
13	22.072749047977275	26.204736177530762	35.52867077110922	16.193844003382747
14	17.61876723273724	30.37692260217877	34.94089369204671	17.063416473037282
15	16.015499246888727	23.746641023630847	43.39021839392974	16.84764133555069
16	18.005914800846686	20.64092021357335	42.72991578164277	18.623249203937196
17	17.88868631706068	21.892169143955176	34.4964996588898	25.722644880094343
18	20.15882891613693	24.449411438951284	36.192776470235	19.19898317467678
19	23.119031613993357	27.05205725313713	30.48777924748921	19.3411318853803
20	23.619070812476124	24.222460563824306	30.613981681798393	21.544486941901177
21	22.145908429008212	27.19383899932112	32.28860758649104	18.37164498517964
22	20.826304016760268	23.09361098091284	31.983960314455235	24.096124687871658
23	18.791619197582037	28.017841147731577	31.47935073968371	21.711188915002676
24	19.327854441855305	25.742060639218835	36.216695884826514	18.713389034099347
25	20.396254960276092	24.658347692210178	34.041930700418504	20.903466647095225
26	17.781732839821654	27.26256144570677	32.34175072100188	22.6139549934697
27	19.16151943327334	25.79026643292139	33.70986117065018	21.338352963155096
28	17.765252796853446	25.519413257093674	36.81835089480962	19.896983051243257
29	18.38945944458252	25.69582310975742	34.78977103082001	21.124946414840046
30	20.420341176921937	25.78299386335242	34.14561485739258	19.65105010233306
31	24.956689846592152	24.50018598429058	29.1164661983977	21.426657970719567
32	23.421410378090215	25.726181083646228	29.615804828252262	21.23660371001129
33	22.009064023632515	25.289660107453884	29.6737852223388	23.0274906465748
34	18.501950750025436	25.540630478404974	32.526033630630195	23.431385140939394
35	18.748951232083375	24.85050365880306	32.99958466288471	23.400960446228854
36	23.13727975873751	25.47264196105839	31.067716629998017	20.322361650206084
37	19.078452010214956	28.368759309639657	30.940346581308496	21.61244209883689
38	19.04632593455021	29.755985274714643	29.795450640770092	21.402238149965054
39	19.4129234895657	26.817400123099915	29.201668687751557	24.56800769958283
40	21.077441189765693	24.753458223591483	31.212000406997014	22.957100179645813
41	18.90984849369405	24.055324905381532	31.10771576262733	25.927110838297086
42	20.34664802931713	27.471497698965656	28.370360609361267	23.811493662355947
43	20.169037201862178	26.762222003526198	29.151561350629596	23.917179443982032
44	20.489397227416255	27.93010326715184	30.033277009839654	21.547222495592255
45	20.054244028069448	29.091946296410587	28.299202852982337	22.554606822537625
46	22.399247389130846	27.804267797362193	29.49884322775319	20.29764158575377
47	20.96498324473364	26.832812632920387	30.24838493910891	21.953819183237062
48	20.306782338331278	26.45687416286219	30.95866144687438	22.277682051932153
49	20.29627380890823	25.336698286776098	31.127431765449625	23.239596138866045
50	19.224303726524695	26.148690687274506	32.88716007826352	21.739845507937275
51	19.013299127792056	27.406444897775362	31.18240972255814	22.397846251874437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2584.0
1	4358.5
2	6133.0
3	62571.5
4	119010.0
5	76496.0
6	33982.0
7	34048.0
8	34114.0
9	35744.5
10	37375.0
11	37288.5
12	37202.0
13	36215.0
14	35228.0
15	32970.5
16	30713.0
17	28592.0
18	26471.0
19	24246.0
20	22021.0
21	21465.5
22	20910.0
23	20771.5
24	20633.0
25	22070.5
26	25117.5
27	26727.0
28	29401.0
29	32075.0
30	36696.0
31	41317.0
32	46174.5
33	51032.0
34	56703.5
35	62375.0
36	70817.5
37	79260.0
38	94756.5
39	110253.0
40	131009.0
41	151765.0
42	169591.5
43	187418.0
44	205774.5
45	224131.0
46	243553.5
47	262976.0
48	258824.0
49	254672.0
50	235349.0
51	216026.0
52	192324.0
53	168622.0
54	152636.0
55	136650.0
56	129891.5
57	123133.0
58	116874.5
59	110616.0
60	101281.5
61	91947.0
62	82674.0
63	73401.0
64	61921.0
65	50441.0
66	41747.0
67	33053.0
68	28316.5
69	23580.0
70	19920.0
71	16260.0
72	12964.5
73	9669.0
74	7231.5
75	3766.0
76	2738.0
77	2149.0
78	1560.0
79	1091.5
80	623.0
81	446.0
82	269.0
83	211.5
84	154.0
85	117.0
86	80.0
87	56.0
88	32.0
89	22.5
90	13.0
91	10.0
92	7.0
93	7.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2997565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.411361598909345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91384863790536	21.568158040164008
2	7.7041770654879524	4.37772320859205
3	3.1889712701995943	2.7180904765852167
4	1.8912367699741113	2.149304469635512
5	1.3238920636062885	1.8806788068522275
6	0.9681664963444426	1.6504157049354662
7	0.7609900474837885	1.5134534388563172
8	0.6425129398146546	1.4603733972041943
9	0.5286393794499971	1.351742810847922
>10	6.326259822201533	39.7911103555597
>50	0.6671797997641761	12.39228122547992
>100	0.07444418183102336	3.3356921482858493
>500	0.005378625520602575	1.0546208666390502
>1k	0.004063850393343944	1.8593397833296468
>5k	0.0	0.0
>10k+	2.390500231378791E-4	2.8970152670328964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54960	1.8334881812404402	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30350	1.0124884698079941	No Hit
CGTTTCTATCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	3614	0.12056452487268833	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6680205433410118E-4	0.0	0.0	0.023419008428507807	0.0
2	1.6680205433410118E-4	0.0	0.0	0.08610322044726303	0.0
3	1.6680205433410118E-4	0.0	0.0	0.1569607331283892	0.0
4	1.6680205433410118E-4	0.0	0.0	0.24056192276064073	0.0
5	1.6680205433410118E-4	0.0	0.0	0.4658781377551446	0.0
6	1.6680205433410118E-4	0.0	0.0	0.8811818926361897	0.0
7	1.6680205433410118E-4	0.0	0.0	1.1764215288075488	0.0
8	1.6680205433410118E-4	0.0	0.0	1.6916730746455872	0.0
9	1.6680205433410118E-4	0.0	0.0	2.42216599139635	0.0
10	1.6680205433410118E-4	0.0	0.0	3.3637635881123513	0.0
11	1.6680205433410118E-4	0.0	0.0	3.876212859437577	0.0
12	2.0016246520092142E-4	0.0	0.0	4.353099932778772	0.0
13	2.0016246520092142E-4	0.0	0.0	4.587556900350784	0.0
14	2.0016246520092142E-4	0.0	0.0	4.696011596078817	0.0
15	2.0016246520092142E-4	0.0	0.0	4.799829194696362	0.0
16	2.0016246520092142E-4	0.0	0.0	4.96666460944133	0.0
17	2.0016246520092142E-4	0.0	0.0	5.148979254828502	0.0
18	2.0016246520092142E-4	0.0	0.0	5.479580926518691	0.0
19	2.0016246520092142E-4	0.0	0.0	5.602847644671591	0.0
20	2.0016246520092142E-4	0.0	0.0	5.739425166760354	0.0
21	2.0016246520092142E-4	0.0	0.0	5.891248396615253	0.0
22	2.0016246520092142E-4	0.0	0.0	6.045139971943894	0.0
23	2.0016246520092142E-4	0.0	0.0	6.231991633208954	0.0
24	2.0016246520092142E-4	0.0	0.0	6.378977603488164	0.0
25	2.0016246520092142E-4	0.0	0.0	6.5034786568431375	0.0
26	2.0016246520092142E-4	0.0	0.0	6.630515101423989	0.0
27	2.0016246520092142E-4	0.0	0.0	6.768226877482223	0.0
28	2.0016246520092142E-4	0.0	0.0	6.911443121333482	0.0
29	2.0016246520092142E-4	0.0	0.0	7.053958796556539	0.0
30	2.0016246520092142E-4	0.0	0.0	7.251318987244647	0.0
31	2.0016246520092142E-4	0.0	0.0	7.401240673680137	0.0
32	2.0016246520092142E-4	0.0	0.0	7.55039507066569	0.0
33	2.0016246520092142E-4	0.0	0.0	7.707155641328878	0.0
34	2.0016246520092142E-4	0.0	0.0	7.8595793585793805	0.0
35	2.0016246520092142E-4	0.0	0.0	8.043428582866426	0.0
36	2.0016246520092142E-4	0.0	0.0	8.191882411223776	0.0
37	2.0016246520092142E-4	0.0	0.0	8.352179185438848	0.0
38	2.0016246520092142E-4	0.0	0.0	8.52925624631993	0.0
39	2.0016246520092142E-4	0.0	0.0	8.72671651824064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33190	0.0	44.06448	1
GTTTTTA	22425	0.0	43.31438	2
TCACGAC	1115	0.0	43.183857	25
CACGACG	1115	0.0	42.78027	26
CGACGGT	1145	0.0	41.855892	28
CTATCTC	2315	0.0	41.59827	6
TCGTTAG	125	0.0	41.4	1
GACGGTC	1180	0.0	40.805084	29
CGGTCTA	1175	0.0	40.787235	31
TATCTCT	2370	0.0	40.63291	7
TTATTTT	24485	0.0	40.359406	6
AGCGATA	385	0.0	40.324673	8
AGACTTA	3360	0.0	40.245537	8
CGCATCG	565	0.0	40.22124	21
TCTCACG	1205	0.0	40.14523	23
TATTTTT	24655	0.0	40.03549	7
CTCACGA	1210	0.0	39.979336	24
ATAACGC	655	0.0	39.84733	11
ACGACGG	1200	0.0	39.75	27
ACGGTCT	1200	0.0	39.75	30
>>END_MODULE