FastQCFastQC Report
Thu 26 May 2016
SRR1546889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546889_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3309248
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376191.1367839460808014No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209360.632651285125805No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39710.11999705068946177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT341350.044.0771941
TTCGCGA1450.043.4482732
ACGTGCG1100.042.9545481
GTTTTTA227550.042.4192472
AGACTTA32200.041.296588
AATCGCG1200.041.2500041
CGCATCG3650.040.68493321
TGCGAGT5700.040.2631574
TTAACGA1850.040.1351362
CGACGGT5500.040.09090828
CTACGCG451.9301297E-840.01
GAGGGGA203800.039.9435737
CGAGGAT4250.039.7058836
TATGCGA2100.039.6428572
CTTGCGA2100.039.6428572
AGCGATC3300.039.5454528
AGGGGAA207300.039.4428338
TAACGCG403.4608456E-739.3751
TTATTTT252000.039.2946436
GCGCGAC9850.039.2893379
Produced by FastQC (version 0.11.5)