##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546889_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3309248
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70449721507726	31.0	31.0	34.0	30.0	34.0
2	32.056141153518865	33.0	31.0	34.0	30.0	34.0
3	31.991029986268785	33.0	31.0	34.0	30.0	34.0
4	35.803018389676446	37.0	35.0	37.0	35.0	37.0
5	35.8225022724196	37.0	35.0	37.0	35.0	37.0
6	35.87531457297851	37.0	35.0	37.0	35.0	37.0
7	36.11967311002379	37.0	35.0	37.0	35.0	37.0
8	13.296638692536794	0.0	0.0	36.0	0.0	37.0
9	24.986928752393293	18.0	17.0	39.0	17.0	39.0
10	32.48101502214401	32.0	32.0	37.0	27.0	39.0
11	35.5443520703193	37.0	34.0	37.0	32.0	39.0
12	36.73618145270466	38.0	35.0	39.0	33.0	39.0
13	36.99488131442551	39.0	37.0	39.0	33.0	39.0
14	38.18646562602742	40.0	37.0	41.0	33.0	41.0
15	38.443841319840644	40.0	38.0	41.0	34.0	41.0
16	38.51258926499313	40.0	38.0	41.0	34.0	41.0
17	38.50801813584234	40.0	38.0	41.0	34.0	41.0
18	38.43863409451332	40.0	38.0	41.0	34.0	41.0
19	38.382063538302354	40.0	37.0	41.0	34.0	41.0
20	38.306054426866766	40.0	37.0	41.0	34.0	41.0
21	38.25355246871797	40.0	37.0	41.0	34.0	41.0
22	38.20489791034096	40.0	37.0	41.0	34.0	41.0
23	38.02127824810954	40.0	36.0	41.0	34.0	41.0
24	37.76429237095557	40.0	35.0	41.0	34.0	41.0
25	37.64960196395072	39.0	35.0	41.0	33.0	41.0
26	37.59503669715899	40.0	35.0	41.0	33.0	41.0
27	37.54171823930996	39.0	35.0	41.0	33.0	41.0
28	37.45226196404742	39.0	35.0	41.0	33.0	41.0
29	37.39200265437948	40.0	35.0	41.0	33.0	41.0
30	37.24462596940453	39.0	35.0	41.0	33.0	41.0
31	37.085984187344074	40.0	35.0	41.0	32.0	41.0
32	36.80326164735916	40.0	35.0	41.0	31.0	41.0
33	36.47532823167076	40.0	35.0	41.0	30.0	41.0
34	36.11722995677568	40.0	35.0	41.0	29.0	41.0
35	35.83745974916356	40.0	35.0	41.0	25.0	41.0
36	35.66490725385344	39.0	35.0	41.0	24.0	41.0
37	35.57888589794418	39.0	35.0	41.0	24.0	41.0
38	35.468339937049144	39.0	35.0	41.0	23.0	41.0
39	35.3816870177152	39.0	35.0	41.0	23.0	41.0
40	35.28944038041271	39.0	35.0	41.0	23.0	41.0
41	35.21153869398727	39.0	35.0	41.0	23.0	41.0
42	35.154288527182004	39.0	35.0	41.0	22.0	41.0
43	35.057141380760825	39.0	35.0	41.0	22.0	41.0
44	34.82756112566964	39.0	35.0	40.0	20.0	41.0
45	34.74903029328718	38.0	34.0	40.0	20.0	41.0
46	34.73872311775968	38.0	34.0	40.0	20.0	41.0
47	34.702972095170864	38.0	34.0	40.0	20.0	41.0
48	34.6186317858317	38.0	34.0	40.0	20.0	41.0
49	34.53436641798983	38.0	34.0	40.0	20.0	41.0
50	34.42895274092483	38.0	34.0	40.0	20.0	41.0
51	34.093402791208156	38.0	34.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	0.0
12	3.0
13	5.0
14	22.0
15	43.0
16	164.0
17	440.0
18	1052.0
19	2356.0
20	4589.0
21	8251.0
22	13624.0
23	22657.0
24	36606.0
25	56873.0
26	70607.0
27	70275.0
28	63326.0
29	60755.0
30	67534.0
31	82058.0
32	104711.0
33	142890.0
34	211028.0
35	318224.0
36	331503.0
37	564518.0
38	745136.0
39	329914.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.872844827586203	22.671041880209643	26.698119935405263	19.757993356798885
2	36.681082832111706	23.202219960546927	28.730787175817586	11.385910031523778
3	27.459592028158664	23.204788519929604	37.05947695669832	12.276142495213413
4	23.722035942909084	25.703211122285186	36.44072611058464	14.134026824221092
5	21.59717252983155	29.999081362291374	34.8530844469801	13.550661660896976
6	19.31082227744793	38.557128386872186	31.896491287446576	10.235558048233315
7	81.5287340205388	3.6454505676214053	13.295800133444214	1.530015278395575
8	31.032971841336764	63.37258494981337	4.74503573017193	0.8494074786779352
9	77.97883386195292	3.996466871023266	14.59443353897925	3.430265728044559
10	35.902839557506724	20.673155955673312	26.007540081613705	17.41646440520626
11	33.27728837488154	20.616194374069273	27.62899607403253	18.47752117701665
12	30.73538157309455	21.519964656622896	30.934308942696347	16.810344827586206
13	23.229023633163788	28.649681136016405	31.796589436633337	16.32470579418647
14	19.342581758756065	32.086141624925055	32.549419082522675	16.021857533796197
15	17.695198425745062	24.35423395284971	42.31323853636839	15.637329085036841
16	19.567587560678437	20.88317345813913	41.2886250894463	18.26061389173613
17	19.480045013247725	20.391581410640725	33.689980321813294	26.43839325429826
18	21.74010530489102	23.740287823698917	36.03368499429478	18.485921877115285
19	24.169448769025472	25.113681416442645	31.726241127893708	18.99062868663817
20	25.114648403504365	24.92451457249502	30.76227590074845	19.19856112325217
21	22.718605556307654	25.48548794167134	33.73026137660278	18.06564512541822
22	20.848180613843386	22.299597975129092	33.63150782292533	23.22071358810219
23	18.902451553948207	27.560672394453363	32.54518851412768	20.99168753747075
24	19.518905805790318	26.209670595857425	36.463903581720075	17.80752001663218
25	19.68676871603458	25.060829529850892	35.291522424429964	19.96087932968457
26	18.456489208424394	28.191268832073025	31.928462297174466	21.42377966232812
27	18.704612044790842	27.25604125166805	34.45492752431972	19.58441917922138
28	16.966815421509658	26.882300752315935	38.09258175875607	18.05830206741834
29	17.93410466667956	26.642790144467867	36.61674797416211	18.80635721469047
30	19.855961233488696	26.16506831763591	35.79704512928617	18.181925319589222
31	24.923547585433305	24.50191100818071	31.005019871584118	19.569521534801865
32	23.94169309764635	26.82032292532926	31.209567853482124	18.028416123542268
33	22.86746112712012	25.88062302976386	30.672859815885662	20.579056027230354
34	19.45599121008761	27.55807361672501	32.033078209913555	20.95285696327383
35	19.694353520799893	26.685216701800528	31.95306003055679	21.667369746842787
36	25.511777902411666	26.821743187576153	28.865409905815458	18.801069004196723
37	20.865374852534472	29.351381341017657	29.59297701471754	20.190266791730327
38	20.81342951631307	31.26686183688862	26.760369727503047	21.15933891929526
39	20.325312578567697	28.554493347129018	28.34486868315702	22.775325391146268
40	21.953446825381477	26.403370191656833	29.442610526621156	22.200572456340534
41	19.560576904481017	25.95586671050341	30.070215348018642	24.413341036996925
42	21.348838165045354	28.274369282688998	27.80677060165935	22.5700219506063
43	21.07025523623494	27.745699325043034	28.034949329877968	23.149096108844063
44	20.838117904732435	28.983019707196316	29.38347322412826	20.795389163942986
45	20.61688939601988	30.15280208675808	27.94553324501518	21.284775272206858
46	22.458576691743865	28.500130543253334	29.255377656796956	19.785915108205852
47	21.966470932368924	28.18812612412246	28.874022134333842	20.97138080917477
48	21.39195974432862	27.974331328446826	29.299254694722183	21.33445423250237
49	21.16713525248032	25.957045225984878	30.64406173245402	22.231757789080785
50	20.324224718123272	28.30860666834278	30.210216943547298	21.156951669986658
51	19.851791101785057	29.672753447308875	28.903877859864235	21.571577591041834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2241.0
1	3618.0
2	4995.0
3	47857.5
4	90720.0
5	61756.0
6	32792.0
7	32741.0
8	32690.0
9	33538.5
10	34387.0
11	34345.5
12	34304.0
13	33205.5
14	32107.0
15	30607.0
16	29107.0
17	27670.5
18	26234.0
19	24691.5
20	23149.0
21	22272.0
22	21395.0
23	21762.0
24	22129.0
25	23727.5
26	28032.0
27	30738.0
28	35477.5
29	40217.0
30	45776.5
31	51336.0
32	60056.5
33	68777.0
34	76759.0
35	84741.0
36	97270.5
37	109800.0
38	132026.0
39	154252.0
40	182462.5
41	210673.0
42	227695.5
43	244718.0
44	255147.5
45	265577.0
46	275619.5
47	285662.0
48	281242.0
49	276822.0
50	263515.0
51	250208.0
52	227685.5
53	205163.0
54	187599.0
55	170035.0
56	153083.5
57	136132.0
58	120440.0
59	104748.0
60	89724.5
61	74701.0
62	63248.5
63	51796.0
64	41255.0
65	30714.0
66	24197.0
67	17680.0
68	14935.5
69	12191.0
70	10380.5
71	8570.0
72	7145.0
73	5720.0
74	4510.5
75	2488.5
76	1676.0
77	1307.0
78	938.0
79	712.5
80	487.0
81	337.0
82	187.0
83	123.0
84	59.0
85	43.5
86	28.0
87	21.5
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3309248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.45787367277369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66629533284744	19.263029878704156
2	7.056913625608797	3.5930803120084827
3	3.0432191040716696	2.3242166253008434
4	1.8155749417265106	1.8488271003970786
5	1.2920628563023542	1.644658648651424
6	1.048384454157649	1.6013783396667136
7	0.8493231210079493	1.5135372505390379
8	0.6830278387281099	1.3910749146662291
9	0.6105051028027979	1.3987945605363612
>10	6.768158907951694	38.714575736060816
>50	0.9899140115795781	16.87307094620757
>100	0.16830824147150805	6.1114020859068345
>500	0.005662111622675422	0.9722141958372452
>1k	0.0024094092011374218	0.9543033727968908
>5k	0.0	0.0
>10k+	2.4094092011374218E-4	1.7958360327202068
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37619	1.1367839460808014	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20936	0.632651285125805	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3971	0.11999705068946177	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007917206567776123	0.0
2	0.0	0.0	0.0	0.03284734175256735	0.0
3	0.0	0.0	0.0	0.05729398340650202	0.0
4	0.0	0.0	0.0	0.08923477478871333	0.0
5	0.0	0.0	0.0	0.17726081575028527	0.0
6	0.0	0.0	0.0	0.2995846790569942	0.0
7	0.0	0.0	0.0	0.3805698454754675	0.0
8	0.0	0.0	0.0	0.5430236718432707	0.0
9	0.0	0.0	0.0	0.7961627535923569	0.0
10	0.0	0.0	0.0	1.1977041309687277	0.0
11	0.0	0.0	0.0	1.4582467074090548	0.0
12	0.0	0.0	0.0	1.700809368170654	0.0
13	0.0	0.0	0.0	1.8021919179221382	0.0
14	0.0	0.0	0.0	1.8499066857485447	0.0
15	0.0	0.0	0.0	1.9037859960933723	0.0
16	0.0	0.0	0.0	2.0130253157212756	0.0
17	0.0	0.0	0.0	2.1377364283365887	0.0
18	0.0	0.0	0.0	2.3418915717407702	0.0
19	0.0	0.0	0.0	2.4262914112209177	0.0
20	0.0	0.0	0.0	2.52483343647862	0.0
21	0.0	0.0	0.0	2.6447398321310462	0.0
22	0.0	0.0	0.0	2.7727447444253195	0.0
23	0.0	0.0	0.0	2.9192432842748564	0.0
24	0.0	0.0	0.0	3.028663913976831	0.0
25	0.0	0.0	0.0	3.130439302222136	0.0
26	0.0	0.0	0.0	3.2323960005415127	0.0
27	0.0	0.0	0.0	3.343085800761986	0.0
28	0.0	0.0	0.0	3.4616021525131995	0.0
29	0.0	0.0	0.0	3.5881263658692246	0.0
30	0.0	0.0	0.0	3.74641006053339	0.0
31	0.0	0.0	0.0	3.881153663913977	0.0
32	0.0	0.0	0.0	4.008070715763823	0.0
33	0.0	0.0	0.0	4.145443315218443	0.0
34	0.0	0.0	0.0	4.2869860463766996	0.0
35	3.0218345678534822E-5	0.0	0.0	4.4488052874852535	0.0
36	3.0218345678534822E-5	0.0	0.0	4.592886359680508	0.0
37	3.0218345678534822E-5	0.0	0.0	4.743434157850968	0.0
38	3.0218345678534822E-5	0.0	0.0	4.908698290366875	0.0
39	3.0218345678534822E-5	0.0	0.0	5.144310731622411	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34135	0.0	44.077194	1
TTCGCGA	145	0.0	43.448273	2
ACGTGCG	110	0.0	42.954548	1
GTTTTTA	22755	0.0	42.419247	2
AGACTTA	3220	0.0	41.29658	8
AATCGCG	120	0.0	41.250004	1
CGCATCG	365	0.0	40.684933	21
TGCGAGT	570	0.0	40.263157	4
TTAACGA	185	0.0	40.135136	2
CGACGGT	550	0.0	40.090908	28
CTACGCG	45	1.9301297E-8	40.0	1
GAGGGGA	20380	0.0	39.943573	7
CGAGGAT	425	0.0	39.705883	6
TATGCGA	210	0.0	39.642857	2
CTTGCGA	210	0.0	39.642857	2
AGCGATC	330	0.0	39.545452	8
AGGGGAA	20730	0.0	39.442833	8
TAACGCG	40	3.4608456E-7	39.375	1
TTATTTT	25200	0.0	39.294643	6
GCGCGAC	985	0.0	39.289337	9
>>END_MODULE