FastQCFastQC Report
Thu 26 May 2016
SRR1546888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546888_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1563555
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140560.8989770107223602No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.5007818720799716No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG25650.16404923395723206No Hit
GAATCTGACTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC23860.1526009638292225No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17330.11083716274771274No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC15920.10181925164129181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTA207.034068E-445.013
ACGCGTA207.034068E-445.021
CGTTTTT111250.043.2202261
GTTTTTA78400.041.900512
CGTTGAG2550.039.7058833
GCGCGAC5300.039.4811339
CGTTAGA1200.039.3750042
CGGTCTA2850.038.6842131
TCGTTAG700.038.571431
CTTGCGA1050.038.571432
TGAATCG356.249733E-638.571431
CGATGAA10700.038.2710319
GAGGGGA92100.038.2573287
AGGGGAA93450.038.1139648
AGACTTA13350.037.921358
CGTAAGA601.5643309E-1037.5000042
TAAGCGA1200.037.5000042
CACGACG3000.037.526
GAGCGAT13500.037.1666687
CGTGGCG5450.037.15596427