##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546888_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1563555
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678261397904134	31.0	31.0	34.0	30.0	34.0
2	32.023802168775646	31.0	31.0	34.0	30.0	34.0
3	31.995709776758734	33.0	31.0	34.0	30.0	34.0
4	35.788097636475854	37.0	35.0	37.0	35.0	37.0
5	35.78489595824899	37.0	35.0	37.0	35.0	37.0
6	35.83637479973522	37.0	35.0	37.0	35.0	37.0
7	36.02277822014576	37.0	35.0	37.0	35.0	37.0
8	13.304596896175703	0.0	0.0	35.0	0.0	37.0
9	24.890104921157235	18.0	17.0	38.0	17.0	39.0
10	32.56544477169015	32.0	32.0	37.0	27.0	39.0
11	35.508191269255	37.0	34.0	37.0	32.0	39.0
12	36.624184630537464	37.0	35.0	39.0	33.0	39.0
13	36.90407628769055	39.0	37.0	39.0	33.0	39.0
14	38.12209036458583	40.0	37.0	41.0	33.0	41.0
15	38.36490817400092	40.0	37.0	41.0	34.0	41.0
16	38.432490702277825	40.0	38.0	41.0	34.0	41.0
17	38.378616678019	40.0	37.0	41.0	34.0	41.0
18	38.31003450470242	40.0	37.0	41.0	34.0	41.0
19	38.267533281528316	40.0	37.0	41.0	34.0	41.0
20	38.188363057263736	40.0	37.0	41.0	34.0	41.0
21	38.165181909174926	40.0	37.0	41.0	34.0	41.0
22	38.08842669429601	40.0	37.0	41.0	33.0	41.0
23	37.92443821931432	40.0	36.0	41.0	33.0	41.0
24	37.78265683010831	40.0	35.0	41.0	33.0	41.0
25	37.678489084170366	40.0	35.0	41.0	33.0	41.0
26	37.58814112711097	40.0	35.0	41.0	33.0	41.0
27	37.509465928605	40.0	35.0	41.0	33.0	41.0
28	37.401565662864435	40.0	35.0	41.0	33.0	41.0
29	37.35970081001308	40.0	35.0	41.0	33.0	41.0
30	37.24807250144702	40.0	35.0	41.0	32.0	41.0
31	37.14703288339713	40.0	35.0	41.0	32.0	41.0
32	36.92127171733645	40.0	35.0	41.0	31.0	41.0
33	36.68983374425588	40.0	35.0	41.0	31.0	41.0
34	36.41972300302835	40.0	35.0	41.0	30.0	41.0
35	36.21255088564202	40.0	35.0	41.0	29.0	41.0
36	36.07847373453444	39.0	35.0	41.0	28.0	41.0
37	35.98961277345536	39.0	35.0	41.0	28.0	41.0
38	35.885551195832576	39.0	35.0	41.0	27.0	41.0
39	35.78677053253643	39.0	35.0	41.0	27.0	41.0
40	35.671504999824116	39.0	35.0	41.0	26.0	41.0
41	35.60164432974855	39.0	35.0	41.0	26.0	41.0
42	35.546144523217926	39.0	35.0	41.0	26.0	41.0
43	35.424615059911545	39.0	35.0	40.0	25.0	41.0
44	35.20513445321719	38.0	35.0	40.0	24.0	41.0
45	35.14860686064769	38.0	35.0	40.0	24.0	41.0
46	35.119400980457996	38.0	34.0	40.0	24.0	41.0
47	35.0631209007678	38.0	34.0	40.0	24.0	41.0
48	34.993787874427184	38.0	34.0	40.0	24.0	41.0
49	34.89428513867437	38.0	34.0	40.0	24.0	41.0
50	34.774621295701145	38.0	34.0	40.0	24.0	41.0
51	34.419107738455	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	3.0
13	8.0
14	13.0
15	37.0
16	94.0
17	274.0
18	569.0
19	1150.0
20	2239.0
21	4040.0
22	6541.0
23	9558.0
24	14175.0
25	20283.0
26	25909.0
27	27418.0
28	27048.0
29	28169.0
30	32186.0
31	40647.0
32	52470.0
33	72017.0
34	107030.0
35	157463.0
36	169858.0
37	276964.0
38	340780.0
39	146574.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.08508175280051	21.964369657607183	26.242185276501306	17.708363313091002
2	36.36309563782534	24.835710927981427	26.27621030280355	12.524983131389686
3	29.575038933711955	24.61435638656779	32.58120117296801	13.229403506752242
4	26.437317523208332	25.247528868507985	33.01143867660555	15.303714931678131
5	24.07417711561154	29.766077944172093	30.64983323260135	15.509911707615018
6	21.2841249588278	39.15557815363067	29.305972607295555	10.254324280245978
7	80.41840549261138	4.860014518197313	12.354474258980336	2.3671057302109615
8	30.18531487539613	64.11958645522543	4.315678054177819	1.3794206152006165
9	75.40943554911723	6.155267963071334	13.16448733814928	5.270809149662147
10	41.717304476017794	25.220858876086865	19.498002948409233	13.563833699486109
11	35.51259789390204	22.478774331571323	25.97535743865742	16.033270335869222
12	30.383581006104677	21.281055031642634	31.158929490807807	17.17643447144488
13	23.281752160940933	27.704685796150443	31.926283373466234	17.087278669442394
14	18.394172254893494	31.998490619133964	32.83402246802959	16.773314657942954
15	17.99271531861687	22.86584098416749	40.99542388978961	18.146019807426025
16	20.08218450901951	21.112784647805803	39.138821467744975	19.66620937542971
17	20.836619114773704	20.956346274995123	31.46259645487367	26.744438155357503
18	22.744642817169847	23.063659417161535	33.615702677552115	20.575995088116503
19	26.390756960900003	23.881155443844314	29.15279603211911	20.57529156313657
20	27.74299592914864	23.794238130414342	28.395739196894258	20.06702674354276
21	24.61889732052918	25.476430314251818	30.557095848882838	19.347576516336172
22	22.779627195717453	22.75314907374541	30.784973985564946	23.682249744972196
23	21.989120945537575	26.39523393804503	29.486394786240332	22.129250330177065
24	21.828461422847294	24.763183898231915	33.258567815011304	20.149786863909487
25	21.475483753369726	24.804052303884415	32.74013386161664	20.98033008112922
26	19.574111559874773	27.216695287341985	30.556840021617404	22.652353131165835
27	21.269350934249196	25.67680701990016	32.09161174375062	20.96223030210002
28	19.054782211051098	26.22901017233164	35.01942688296862	19.69678073364864
29	20.473792095577068	25.462295857836786	34.180697193255114	19.88321485333103
30	21.616956231152727	25.782847421421057	32.24350918259991	20.356687164826308
31	26.836855754994225	24.305189136295173	27.814563606652786	21.04339150205781
32	27.22302701216139	25.53987547607855	28.30082728141959	18.936270230340472
33	25.59238402230814	25.55490532792259	28.233033056080536	20.61967759368874
34	22.15515284080189	26.27358807333289	30.34226490273767	21.228994183127554
35	22.820303730920884	25.72202448906498	30.01250355759791	21.445168222416225
36	26.32161964241744	26.538049508971547	27.41553702939775	19.724793819213268
37	23.27753101106133	28.80736526697174	27.824540870004572	20.090562851962353
38	22.699489304821384	30.155063301258988	25.466836791798176	21.67861060212145
39	23.4305157157887	27.332009427234734	26.732158446616843	22.505316410359725
40	22.560191358794544	26.064641154292623	28.35685345254884	23.018314034364
41	20.84397414865483	25.31244503711094	28.53510109973746	25.308479714496773
42	22.544969636501435	26.762282107121273	26.637438401591247	24.055309854786046
43	22.48843181084132	25.97529348184106	27.632158766400927	23.904115940916693
44	21.59898436575624	27.795824259460012	28.78478851079751	21.820402863986235
45	21.123017738423016	28.5977787797679	27.163419259316107	23.115784222492973
46	23.48212886658928	28.17009954878466	28.290210449904222	20.057561134721837
47	22.73127584255111	26.881433655995473	28.978321837095596	21.408968664357825
48	22.986399583001557	25.859979341948318	29.321130372772302	21.83249070227782
49	21.837159549871927	25.12415616975418	30.781072619767137	22.257611660606756
50	21.13165190863129	27.568905475023264	29.354963528625472	21.94447908771997
51	21.033350281889668	28.5578057695444	27.907684731269445	22.501159217296482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1256.0
1	2499.5
2	3743.0
3	19843.5
4	35944.0
5	24117.0
6	12290.0
7	11893.0
8	11496.0
9	11589.0
10	11682.0
11	11451.0
12	11220.0
13	10878.5
14	10537.0
15	10020.0
16	9503.0
17	9056.5
18	8610.0
19	8503.0
20	8396.0
21	8331.0
22	8266.0
23	8956.0
24	9646.0
25	11020.5
26	14280.0
27	16165.0
28	18422.0
29	20679.0
30	23140.0
31	25601.0
32	28240.0
33	30879.0
34	34451.0
35	38023.0
36	41872.0
37	45721.0
38	55483.0
39	65245.0
40	77802.5
41	90360.0
42	97055.5
43	103751.0
44	108067.0
45	112383.0
46	118441.0
47	124499.0
48	118803.0
49	113107.0
50	109389.0
51	105671.0
52	100077.5
53	94484.0
54	90456.5
55	86429.0
56	82337.0
57	78245.0
58	73785.0
59	69325.0
60	63498.5
61	57672.0
62	50431.5
63	43191.0
64	36530.0
65	29869.0
66	25049.0
67	20229.0
68	17305.0
69	14381.0
70	12059.0
71	9737.0
72	8109.0
73	6481.0
74	4989.5
75	2550.5
76	1603.0
77	1183.0
78	763.0
79	533.0
80	303.0
81	225.0
82	147.0
83	104.5
84	62.0
85	49.0
86	36.0
87	28.0
88	20.0
89	14.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1563555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.952806697587196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81390612274073	19.625391366224914
2	6.130122381395553	3.3044800715661444
3	2.9762489218484336	2.4065478562344937
4	1.9392842113955606	2.090766099257095
5	1.5632020623516256	2.106634150791651
6	1.3472810836309208	2.178780396865201
7	1.0889338221676945	2.054487597074546
8	0.998234498338405	2.1524177178062365
9	0.8756177162851727	2.1240319543215302
>10	9.97868450687812	53.502104255802095
>50	0.24660939466175832	4.108773471253854
>100	0.03826534176728896	1.9527048413403298
>500	0.0019252994873125158	0.35027251818636124
>1k	0.0012033121795703222	0.622966160122558
>5k	2.4066243591406447E-4	0.5078951513702238
>10k+	2.4066243591406447E-4	0.9117463917828691
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14056	0.8989770107223602	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7830	0.5007818720799716	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	2565	0.16404923395723206	No Hit
GAATCTGACTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	2386	0.1526009638292225	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1733	0.11083716274771274	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	1592	0.10181925164129181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.395681635759536E-5	0.0	0.0	0.005820070288541177	0.0
2	6.395681635759536E-5	0.0	0.0	0.029356178708136265	0.0
3	6.395681635759536E-5	0.0	0.0	0.04911883496263323	0.0
4	6.395681635759536E-5	0.0	0.0	0.10501709245917157	0.0
5	6.395681635759536E-5	0.0	0.0	0.26337416976057765	0.0
6	6.395681635759536E-5	0.0	0.0	0.3744032029573632	0.0
7	6.395681635759536E-5	0.0	0.0	0.459145984631177	0.0
8	6.395681635759536E-5	0.0	0.0	0.6061187486209312	0.0
9	6.395681635759536E-5	0.0	0.0	0.8176878971318565	0.0
10	1.279136327151907E-4	0.0	0.0	1.2980035879773977	0.0
11	1.279136327151907E-4	0.0	0.0	1.4815596509236963	0.0
12	1.279136327151907E-4	0.0	0.0	1.7542715158724829	0.0
13	1.279136327151907E-4	0.0	0.0	1.8521254448996038	0.0
14	1.279136327151907E-4	0.0	0.0	1.8944648573283318	0.0
15	1.279136327151907E-4	0.0	0.0	1.948956064865003	0.0
16	1.279136327151907E-4	0.0	0.0	2.0249367626978265	0.0
17	1.279136327151907E-4	0.0	0.0	2.1030280354704503	0.0
18	1.279136327151907E-4	0.0	0.0	2.241686413333717	0.0
19	1.279136327151907E-4	0.0	0.0	2.312166824959787	0.0
20	1.279136327151907E-4	0.0	0.0	2.3789377412371167	0.0
21	1.279136327151907E-4	0.0	0.0	2.454023043640934	0.0
22	1.279136327151907E-4	0.0	0.0	2.536975034456735	0.0
23	1.279136327151907E-4	0.0	0.0	2.619415370741675	0.0
24	1.279136327151907E-4	0.0	0.0	2.6922621845729764	0.0
25	1.279136327151907E-4	0.0	0.0	2.7566666986450747	0.0
26	1.279136327151907E-4	0.0	0.0	2.815890710592208	0.0
27	1.279136327151907E-4	0.0	0.0	2.8876502585454302	0.0
28	1.279136327151907E-4	0.0	0.0	2.95947376331501	0.0
29	1.279136327151907E-4	0.0	0.0	3.0353265475151177	0.0
30	1.279136327151907E-4	0.0	0.0	3.135482921931112	0.0
31	1.279136327151907E-4	0.0	0.0	3.2242549830354545	0.0
32	1.279136327151907E-4	0.0	0.0	3.3120676918944327	0.0
33	1.279136327151907E-4	0.0	0.0	3.4028863711222184	0.0
34	1.279136327151907E-4	0.0	0.0	3.4949841866771556	0.0
35	1.279136327151907E-4	0.0	0.0	3.5975709201147383	0.0
36	1.279136327151907E-4	0.0	0.0	3.6901803902005366	0.0
37	1.279136327151907E-4	0.0	0.0	3.7815107239591828	0.0
38	1.279136327151907E-4	0.0	0.0	3.890876879930671	0.0
39	1.279136327151907E-4	0.0	0.0	4.033628494040824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATTA	20	7.034068E-4	45.0	13
ACGCGTA	20	7.034068E-4	45.0	21
CGTTTTT	11125	0.0	43.220226	1
GTTTTTA	7840	0.0	41.90051	2
CGTTGAG	255	0.0	39.705883	3
GCGCGAC	530	0.0	39.481133	9
CGTTAGA	120	0.0	39.375004	2
CGGTCTA	285	0.0	38.68421	31
TCGTTAG	70	0.0	38.57143	1
CTTGCGA	105	0.0	38.57143	2
TGAATCG	35	6.249733E-6	38.57143	1
CGATGAA	1070	0.0	38.27103	19
GAGGGGA	9210	0.0	38.257328	7
AGGGGAA	9345	0.0	38.113964	8
AGACTTA	1335	0.0	37.92135	8
CGTAAGA	60	1.5643309E-10	37.500004	2
TAAGCGA	120	0.0	37.500004	2
CACGACG	300	0.0	37.5	26
GAGCGAT	1350	0.0	37.166668	7
CGTGGCG	545	0.0	37.155964	27
>>END_MODULE