FastQCFastQC Report
Thu 26 May 2016
SRR1546887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546887_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2513833
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344091.3687862320209816No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191680.7625009298549267No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27600.10979249616024613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT259450.043.4390071
CGGTCTA5750.043.04347631
GTTTTTA179700.042.0826382
TATGCGA2150.040.8139532
CGACGGT6150.040.60975628
ACGGTCT6250.039.9630
GAGCGAT22500.039.37
CACGACG6450.039.06976726
TAACGCC2550.038.8235312
TTATTTT203150.038.742316
TATTTTT204150.038.4753887
ACGACGG6500.038.42307727
TTTTATT207250.038.203864
TTTATTT208150.037.887345
AGACTTA18400.037.7853248
TTTTTAT209850.037.6125833
TCACGAC6800.037.05882325
ATACGAG4250.037.0588233
ATAACGC2750.036.81818411
GACGGTC6800.036.7279429