##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546887_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2513833
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68233251771299	31.0	31.0	34.0	30.0	34.0
2	32.02597030112979	31.0	31.0	34.0	30.0	34.0
3	31.944866663775993	31.0	31.0	34.0	30.0	34.0
4	35.77119084680645	37.0	35.0	37.0	35.0	37.0
5	35.79112017385403	37.0	35.0	37.0	35.0	37.0
6	35.84242787806509	37.0	35.0	37.0	35.0	37.0
7	36.07985534440832	37.0	35.0	37.0	35.0	37.0
8	13.264465857517186	0.0	0.0	35.0	0.0	37.0
9	24.891141137856014	18.0	17.0	38.0	17.0	39.0
10	32.6022361071718	32.0	32.0	37.0	27.0	39.0
11	35.52624020768285	37.0	35.0	37.0	32.0	39.0
12	36.68466123246851	38.0	35.0	39.0	33.0	39.0
13	36.99039037199368	39.0	37.0	39.0	33.0	39.0
14	38.201064668973636	40.0	37.0	41.0	33.0	41.0
15	38.42291194363349	40.0	38.0	41.0	34.0	41.0
16	38.47323867575929	40.0	38.0	41.0	34.0	41.0
17	38.426248680799404	40.0	38.0	41.0	34.0	41.0
18	38.33512767156768	40.0	37.0	41.0	34.0	41.0
19	38.28794474414172	40.0	37.0	41.0	34.0	41.0
20	38.225581412926	40.0	37.0	41.0	34.0	41.0
21	38.17184872662583	40.0	37.0	41.0	34.0	41.0
22	38.11631202231811	40.0	37.0	41.0	33.0	41.0
23	37.93280579895323	39.0	36.0	41.0	33.0	41.0
24	37.678356915515074	39.0	35.0	41.0	33.0	41.0
25	37.566220986040044	39.0	35.0	41.0	33.0	41.0
26	37.49371696528767	39.0	35.0	41.0	33.0	41.0
27	37.44682045306908	39.0	35.0	41.0	33.0	41.0
28	37.29888898745462	39.0	35.0	41.0	33.0	41.0
29	37.24044874898213	39.0	35.0	41.0	33.0	41.0
30	37.07097090379512	39.0	35.0	41.0	32.0	41.0
31	36.92143193282927	39.0	35.0	41.0	32.0	41.0
32	36.63718114926489	39.0	35.0	41.0	31.0	41.0
33	36.324052950215865	39.0	35.0	41.0	30.0	41.0
34	35.950651455367165	39.0	35.0	41.0	27.0	41.0
35	35.69835903976119	39.0	35.0	41.0	25.0	41.0
36	35.54161155494418	39.0	35.0	41.0	24.0	41.0
37	35.49207524923096	39.0	35.0	41.0	24.0	41.0
38	35.38025954786973	39.0	35.0	41.0	23.0	41.0
39	35.27566349872883	39.0	35.0	41.0	23.0	41.0
40	35.17052922767741	39.0	35.0	41.0	23.0	41.0
41	35.07389313450814	39.0	35.0	40.0	23.0	41.0
42	35.002343831113684	39.0	35.0	40.0	22.0	41.0
43	34.8832273265567	38.0	34.0	40.0	22.0	41.0
44	34.6939617707302	38.0	34.0	40.0	21.0	41.0
45	34.60630280531762	38.0	34.0	40.0	20.0	41.0
46	34.55707200915892	38.0	34.0	40.0	20.0	41.0
47	34.51566671294394	38.0	34.0	40.0	20.0	41.0
48	34.419356417073054	38.0	34.0	40.0	20.0	41.0
49	34.30868717213912	38.0	34.0	40.0	20.0	41.0
50	34.189030456677116	38.0	34.0	40.0	20.0	41.0
51	33.820832967026845	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	2.0
13	10.0
14	25.0
15	57.0
16	153.0
17	399.0
18	997.0
19	1938.0
20	3768.0
21	6452.0
22	10252.0
23	16236.0
24	26134.0
25	39943.0
26	52599.0
27	55605.0
28	52539.0
29	52142.0
30	55998.0
31	67429.0
32	84687.0
33	113829.0
34	167926.0
35	242655.0
36	264130.0
37	436319.0
38	526580.0
39	234983.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.326557492084795	20.508204005596234	25.364692085751123	20.800546416567848
2	38.90624397086043	22.032808066406957	26.499373665633318	12.56157429709929
3	27.933796716010967	23.3663493159649	35.8291899262998	12.87066404172433
4	25.09367169577295	25.14486841409115	34.93999004707155	14.821469843064355
5	21.645192819093392	29.351551992515017	34.45952853670073	14.543726651690864
6	20.01087582190225	37.40013755885932	32.428844716415135	10.160141902823298
7	79.24846240780514	4.265518035605388	14.363921549283504	2.1220980073059748
8	29.808901386846305	63.6531145863707	5.354333402417742	1.183650624365262
9	73.95972604385415	5.71812049567334	16.281789601775458	4.040363858697058
10	38.02189723820158	26.945743810348578	22.09204032248761	12.940318628962228
11	31.808875132118956	22.50495557978593	30.015478355165197	15.670690932929912
12	28.311427210956335	21.054859252782503	34.256094179684965	16.377619356576194
13	23.261489526153888	24.958897428747257	34.7296339892109	17.04997905588796
14	18.711624837449424	28.39134500979182	35.796331737231554	17.100698415527205
15	18.249899655227694	22.99321394857972	42.23693459350721	16.51995180268538
16	21.65378527531463	21.265812008991848	39.903724710432236	17.17667800526129
17	20.818964505597627	21.049051388855187	34.55380687579485	23.578177229752335
18	22.684044644174854	22.60921867124825	36.311680211056185	18.395056473520714
19	24.671487724124873	24.49876344212205	32.41889974393685	18.410849089816228
20	25.806726222465855	23.957398920294228	31.52114718837727	18.71472766886265
21	24.205506093682434	24.8036365184163	33.419682214371434	17.571175173529824
22	22.299452668494684	22.372767005604587	33.79468723658255	21.533093089318182
23	20.605426056543934	25.338198679068974	33.234228367596415	20.822146896790677
24	21.196833679882474	23.477454548492283	36.24918600400265	19.07652576762259
25	21.14547784200462	23.430991637073745	34.42432333412761	20.99920718679403
26	19.17211684308385	25.76109868873549	33.14054672685099	21.926237741329675
27	19.72223294069256	24.596144612629402	34.85792413418075	20.823698312497292
28	18.845961525686075	24.84488826425622	36.861358729875846	19.44779148018186
29	20.686656591746548	24.793293747038884	35.22000069217008	19.300048969044482
30	22.176532808663104	24.085887964713645	34.93700655532806	18.80057267129519
31	25.972886822633008	23.010319301242365	31.388481255516975	19.628312620607655
32	25.8262581484132	24.42525020556258	31.554084937225348	18.194406708798873
33	24.853639840037108	24.04340304228642	31.038736463400713	20.06422065427576
34	21.227185735886195	25.95335489668566	32.9948727699891	19.82458659743905
35	21.167237441787105	26.07170006917723	32.33571999412849	20.425342494907177
36	23.878157379587268	27.097862109376397	29.759415203794365	19.26456530724197
37	22.224268676558864	28.531489561955787	29.585298625644583	19.658943135840765
38	21.429347136424735	29.606660426527938	28.08877916711253	20.875213269934797
39	21.36438657619659	27.690025550623293	29.002125439518057	21.943462433662063
40	22.094148656653008	25.193757898794388	30.201489120399007	22.510604324153594
41	19.938794661379657	25.82995767817512	29.911095924033138	24.320151736412086
42	21.4970922889468	26.867894565788582	28.56892243836404	23.066090706900578
43	21.208648307186674	26.172502310217105	29.210293603433477	23.408555779162736
44	21.05111994313067	26.88392586142357	30.351021726582474	21.713932468863288
45	21.21393903254512	28.100355115077253	29.072058485985348	21.61364736639228
46	22.0795892169448	27.221060428437372	30.196874653169086	20.502475701448745
47	21.452499032354176	26.45458150959113	30.338968419938794	21.753951038115897
48	21.198663554818477	25.77796536205866	31.625927418408462	21.3974436647144
49	21.77801787151334	24.521716438601928	31.683608258782503	22.016657431102225
50	20.218646186918544	26.406050043897107	31.716267548401188	21.65903622078316
51	20.25989793275846	26.754402539866412	31.232583866947405	21.75311566042772
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2079.0
1	3891.0
2	5703.0
3	46177.5
4	86652.0
5	57571.0
6	28490.0
7	27695.0
8	26900.0
9	26702.5
10	26505.0
11	25732.5
12	24960.0
13	23783.0
14	22606.0
15	21321.0
16	20036.0
17	18952.0
18	17868.0
19	16988.5
20	16109.0
21	16484.0
22	16859.0
23	17619.5
24	18380.0
25	19970.5
26	24397.5
27	27234.0
28	30710.0
29	34186.0
30	39253.5
31	44321.0
32	49702.5
33	55084.0
34	60354.0
35	65624.0
36	72776.0
37	79928.0
38	91426.0
39	102924.0
40	118043.0
41	133162.0
42	143262.0
43	153362.0
44	159997.0
45	166632.0
46	177066.0
47	187500.0
48	187638.0
49	187776.0
50	179144.5
51	170513.0
52	158663.0
53	146813.0
54	137664.0
55	128515.0
56	122871.5
57	117228.0
58	111828.5
59	106429.0
60	96765.0
61	87101.0
62	76412.0
63	65723.0
64	55070.0
65	44417.0
66	36213.0
67	28009.0
68	23038.0
69	18067.0
70	15138.5
71	12210.0
72	10074.5
73	7939.0
74	6110.5
75	3260.0
76	2238.0
77	1652.0
78	1066.0
79	750.5
80	435.0
81	325.5
82	216.0
83	159.5
84	103.0
85	69.5
86	36.0
87	30.0
88	24.0
89	17.5
90	11.0
91	9.5
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2513833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.2751833847273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45504609713038	20.769548989299587
2	6.5003605667750275	3.675977741848276
3	3.001455834038486	2.5460014238579345
4	2.0495938716375504	2.3181057033905987
5	1.5081298289851512	2.1321323741266283
6	1.3105318971444564	2.223331783397645
7	1.0488704841178724	2.0759903699682383
8	0.9310540755459882	2.106057978172845
9	0.878766336503018	2.2362551385253036
>10	9.03259954574349	50.515390765676436
>50	0.24410556810432515	4.2917319594509555
>100	0.03548157945406276	1.9917664300264821
>500	0.0028602248715416945	0.5966838180357392
>1k	8.580674614625072E-4	0.3545489100391269
>5k	0.0	0.0
>10k+	2.8602248715416903E-4	2.166476614184113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34409	1.3687862320209816	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19168	0.7625009298549267	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2760	0.10979249616024613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.95597798262653E-5	0.0	0.0	0.010581450716893285	0.0
2	7.95597798262653E-5	0.0	0.0	0.033812906426162755	0.0
3	7.95597798262653E-5	0.0	0.0	0.06336936463162032	0.0
4	1.1933966973939796E-4	0.0	0.0	0.09137440713046571	0.0
5	1.1933966973939796E-4	0.0	0.0	0.1722469233238644	0.0
6	1.1933966973939796E-4	0.0	0.0	0.32352984466350787	0.0
7	1.1933966973939796E-4	0.0	0.0	0.429423911612267	0.0
8	1.1933966973939796E-4	0.0	0.0	0.6406153471610883	0.0
9	1.1933966973939796E-4	0.0	0.0	1.01295511674801	0.0
10	1.1933966973939796E-4	0.0	0.0	1.5087716646252953	0.0
11	1.1933966973939796E-4	0.0	0.0	1.7875889130264422	0.0
12	1.1933966973939796E-4	0.0	0.0	2.028137907331155	0.0
13	1.1933966973939796E-4	0.0	0.0	2.149745030795602	0.0
14	1.1933966973939796E-4	0.0	0.0	2.2111254009315653	0.0
15	1.1933966973939796E-4	0.0	0.0	2.264430453415163	0.0
16	1.1933966973939796E-4	0.0	0.0	2.355247942086845	0.0
17	1.1933966973939796E-4	0.0	0.0	2.4459858709787006	0.0
18	1.1933966973939796E-4	0.0	0.0	2.6250351554777107	0.0
19	1.1933966973939796E-4	0.0	0.0	2.694093044366909	0.0
20	1.1933966973939796E-4	0.0	0.0	2.7788639897717946	0.0
21	1.1933966973939796E-4	0.0	0.0	2.870039497452695	0.0
22	1.1933966973939796E-4	0.0	0.0	2.971239537391704	0.0
23	1.1933966973939796E-4	0.0	0.0	3.0894653702135346	0.0
24	1.1933966973939796E-4	0.0	0.0	3.1778165057106023	0.0
25	1.1933966973939796E-4	0.0	0.0	3.256859146967997	0.0
26	1.1933966973939796E-4	0.0	0.0	3.3370951849227852	0.0
27	1.1933966973939796E-4	0.0	0.0	3.423457325924196	0.0
28	1.1933966973939796E-4	0.0	0.0	3.508546510448387	0.0
29	1.1933966973939796E-4	0.0	0.0	3.602665729982859	0.0
30	1.1933966973939796E-4	0.0	0.0	3.7323481710996713	0.0
31	1.1933966973939796E-4	0.0	0.0	3.8286950644692785	0.0
32	1.591195596525306E-4	0.0	0.0	3.937333943822044	0.0
33	1.591195596525306E-4	0.0	0.0	4.04903587469812	0.0
34	1.591195596525306E-4	0.0	0.0	4.16141406370272	0.0
35	1.591195596525306E-4	0.0	0.0	4.282901847497428	0.0
36	1.591195596525306E-4	0.0	0.0	4.405264788870223	0.0
37	1.591195596525306E-4	0.0	0.0	4.52014911093935	0.0
38	1.591195596525306E-4	0.0	0.0	4.685713012757809	0.0
39	1.9889944956566328E-4	0.0	0.0	4.878009000597892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25945	0.0	43.439007	1
CGGTCTA	575	0.0	43.043476	31
GTTTTTA	17970	0.0	42.082638	2
TATGCGA	215	0.0	40.813953	2
CGACGGT	615	0.0	40.609756	28
ACGGTCT	625	0.0	39.96	30
GAGCGAT	2250	0.0	39.3	7
CACGACG	645	0.0	39.069767	26
TAACGCC	255	0.0	38.82353	12
TTATTTT	20315	0.0	38.74231	6
TATTTTT	20415	0.0	38.475388	7
ACGACGG	650	0.0	38.423077	27
TTTTATT	20725	0.0	38.20386	4
TTTATTT	20815	0.0	37.88734	5
AGACTTA	1840	0.0	37.785324	8
TTTTTAT	20985	0.0	37.612583	3
TCACGAC	680	0.0	37.058823	25
ATACGAG	425	0.0	37.058823	3
ATAACGC	275	0.0	36.818184	11
GACGGTC	680	0.0	36.72794	29
>>END_MODULE