FastQCFastQC Report
Thu 26 May 2016
SRR1546886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546886_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2971965
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347371.1688226476422166No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188970.6358419429569325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG302.1666692E-644.9999968
CGTTTTT338800.043.7979661
GTTTTTA230400.042.675782
GAGCGAT31350.039.9760787
CGGTCTA4400.039.37531
TATTTTT262950.038.736457
TTATTTT264100.038.6018566
CGACGGT4600.038.15217228
ACGGTCT4500.038.030
TTTTATT268800.037.92694
TAATGCG950.037.8947371
CCGCTCG1800.037.519
TTTATTT272250.037.4297525
GTTGCGA2000.037.1252
CACGACG4700.036.86170226
TTTTTAT276650.036.7693863
TTAGCGA3400.036.3970572
TCGCCGT1300.036.34615325
AGCGATA6200.036.290328
TCACGAC4850.036.1855725
Produced by FastQC (version 0.11.5)