##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546886_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2971965 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.660958658665226 31.0 31.0 34.0 30.0 34.0 2 32.00629482514094 31.0 31.0 34.0 30.0 34.0 3 31.91924602073039 31.0 31.0 34.0 30.0 34.0 4 35.74867032417946 37.0 35.0 37.0 35.0 37.0 5 35.77327929501189 37.0 35.0 37.0 35.0 37.0 6 35.82422269441262 37.0 35.0 37.0 35.0 37.0 7 36.05467762911071 37.0 35.0 37.0 35.0 37.0 8 13.3412819464563 0.0 0.0 35.0 0.0 37.0 9 24.93420447414421 18.0 17.0 38.0 17.0 39.0 10 32.5339749963408 32.0 32.0 37.0 27.0 39.0 11 35.49398697494755 37.0 34.0 37.0 32.0 39.0 12 36.7058821352203 38.0 35.0 39.0 33.0 39.0 13 37.03506703477329 39.0 37.0 39.0 33.0 39.0 14 38.268203023925246 40.0 38.0 41.0 34.0 41.0 15 38.477447076260994 40.0 38.0 41.0 34.0 41.0 16 38.50837610806318 40.0 38.0 41.0 34.0 41.0 17 38.45893474519384 40.0 38.0 41.0 34.0 41.0 18 38.36373577750747 40.0 38.0 41.0 34.0 41.0 19 38.31197238190894 40.0 37.0 41.0 34.0 41.0 20 38.23106564175554 40.0 37.0 41.0 34.0 41.0 21 38.18878587062768 40.0 37.0 41.0 34.0 41.0 22 38.12306638873607 40.0 37.0 41.0 33.0 41.0 23 37.9207426736183 39.0 36.0 41.0 33.0 41.0 24 37.6414032466735 39.0 35.0 41.0 33.0 41.0 25 37.53237067058327 39.0 35.0 41.0 33.0 41.0 26 37.45823217971948 39.0 35.0 41.0 33.0 41.0 27 37.40197714306864 39.0 35.0 41.0 33.0 41.0 28 37.266909267101056 39.0 35.0 41.0 33.0 41.0 29 37.19502955115555 39.0 35.0 41.0 33.0 41.0 30 37.02816116609718 39.0 35.0 41.0 32.0 41.0 31 36.86617103498863 39.0 35.0 41.0 31.0 41.0 32 36.53894477222982 39.0 35.0 41.0 30.0 41.0 33 36.1752826833425 39.0 35.0 41.0 29.0 41.0 34 35.78573805546162 39.0 35.0 41.0 25.0 41.0 35 35.488320690183095 39.0 35.0 41.0 23.0 41.0 36 35.307692048863295 39.0 35.0 41.0 23.0 41.0 37 35.23907784916713 39.0 35.0 41.0 22.0 41.0 38 35.1158115926668 39.0 35.0 41.0 21.0 41.0 39 35.00410839293195 39.0 35.0 41.0 21.0 41.0 40 34.90034438494397 39.0 35.0 40.0 20.0 41.0 41 34.80242263956675 39.0 35.0 40.0 20.0 41.0 42 34.72238502135792 39.0 34.0 40.0 18.0 41.0 43 34.61248365980084 38.0 34.0 40.0 18.0 41.0 44 34.43070190934281 38.0 34.0 40.0 18.0 41.0 45 34.34186741768493 38.0 34.0 40.0 18.0 41.0 46 34.29443617270055 38.0 34.0 40.0 18.0 41.0 47 34.23735239143126 38.0 34.0 40.0 18.0 41.0 48 34.15993156043224 38.0 34.0 40.0 18.0 41.0 49 34.06054108981768 38.0 33.0 40.0 18.0 41.0 50 33.95425450838082 38.0 33.0 40.0 18.0 41.0 51 33.61014985035153 37.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 6.0 12 6.0 13 10.0 14 32.0 15 56.0 16 187.0 17 486.0 18 1235.0 19 2578.0 20 4859.0 21 8501.0 22 13941.0 23 22048.0 24 35182.0 25 53522.0 26 68847.0 27 71088.0 28 65580.0 29 61986.0 30 66435.0 31 78886.0 32 98991.0 33 131323.0 34 188637.0 35 270501.0 36 309690.0 37 511328.0 38 620851.0 39 285123.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.5987806047514 21.0236661602677 24.51230078416132 20.865252450819575 2 40.27789694696943 22.627520849000575 24.91254102925169 12.182041174778304 3 28.551009180794523 23.129680194753302 35.791740481465965 12.527570142986205 4 24.70032453275863 24.672800655458595 35.81455367071954 14.812321141063236 5 22.36392420502933 28.53199818974988 34.575205293467455 14.528872311753336 6 19.716349284059536 37.55279755986359 33.0441307350524 9.686722421024474 7 78.45735060809935 3.9368565915143683 15.573198203881944 2.032594596504333 8 29.55092001419936 63.59233705645928 5.645524089280998 1.211218840060364 9 73.01815465525334 5.432365455178644 17.1397375137325 4.409742375835516 10 34.47301701063101 26.242435560311105 24.70762609923064 14.576921329827236 11 29.5337932983733 23.6773649756979 30.559983041523033 16.228858684405772 12 26.378439853766782 22.033805916287708 34.8700943651759 16.717659864769605 13 22.744413208096326 25.37526518650119 35.34691693879302 16.533404666609467 14 19.018864623237487 29.069824173568666 35.42646027123469 16.48485093195916 15 18.981448301039887 23.7086574034351 40.907345813291876 16.402548482233133 16 20.763636180103063 22.166041659306217 39.29955433526303 17.770767825327688 17 20.876827284305165 21.748775641705066 35.14472747828457 22.229669595705197 18 22.637480589441665 23.081765767766445 35.957590348473154 18.323163294318743 19 24.268455382213453 24.411593003282338 32.7476602180712 18.572291396433 20 25.6968032934439 24.189113936402347 31.838430129560745 18.275652640593005 21 23.946547149781374 25.061331475976335 33.71079403694189 17.281327337300407 22 21.87434912591501 22.396528895865195 34.37792840763603 21.35119357058377 23 20.50549047515701 26.01645712516803 33.513281616708134 19.964770782966827 24 20.690721458698203 25.141244933907362 35.86341023531569 18.304623372078744 25 20.791597478435982 24.796994581026357 34.874098450015396 19.537309490522265 26 19.44471082263755 26.35791471299292 33.775027633232554 20.422346831136977 27 20.106596140937057 25.093364154692267 35.05408038116195 19.74595932320872 28 18.78820914781971 25.629541397694794 37.06931272743791 18.512936727047595 29 19.82186869630026 25.108741186386784 35.81317411207736 19.256216005235594 30 21.20684462973151 24.896491042121962 35.45596936706859 18.44069496107794 31 24.48114294751116 23.94382168026878 31.839876983746446 19.73515838847362 32 23.894763229042066 24.957999168900038 32.288368133541276 18.85886946851662 33 23.384831248012677 24.74995499610527 31.474024761395235 20.39118899448681 34 20.3297145154805 27.00442299959791 32.57185061062294 20.094011874298655 35 21.07868026709601 26.691431426682342 31.93042313755377 20.29946516866787 36 23.70566275174842 27.291943209290825 30.4057416557732 18.596652383187553 37 21.755808026003 28.546735913780953 29.871818813478622 19.82563724673743 38 21.1937556465167 29.72050478387195 28.796705210189216 20.289034359422136 39 21.76765204166267 27.81516606016558 28.607672028439097 21.80950986973265 40 22.183740387252204 26.05696904236759 30.306211546905836 21.453079023474366 41 20.585774058577407 26.419591078629796 29.863339574994995 23.131295287797805 42 21.81257181696285 27.55257884934715 28.82715644363241 21.807692890057588 43 21.918091229203572 27.03204782021323 29.028336470988048 22.02152447959515 44 21.557891832508123 27.848847479697774 29.816905649965598 20.776355037828505 45 21.13581418354523 28.397810875969263 29.135134498555672 21.331240441929832 46 22.45352822122737 27.663044484036657 29.940426620098147 19.943000674637823 47 21.712402400432037 27.448001574715718 30.147259473109543 20.692336551742702 48 21.520340919223475 26.866971851956535 30.51866357780122 21.09402365101877 49 21.491706665455347 25.733041943629885 31.524260884633566 21.250990506281198 50 20.45279806458017 26.997256024212934 31.71803840220191 20.831907509004985 51 20.684631212009563 27.777110430304532 30.131243133751575 21.407015223934334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1767.0 1 3641.5 2 5516.0 3 49016.0 4 92516.0 5 63984.0 6 35452.0 7 34698.0 8 33944.0 9 33932.0 10 33920.0 11 33573.0 12 33226.0 13 32007.0 14 30788.0 15 29373.0 16 27958.0 17 26003.0 18 24048.0 19 23008.0 20 21968.0 21 21656.0 22 21344.0 23 22693.5 24 24043.0 25 25928.5 26 31354.0 27 34894.0 28 38473.0 29 42052.0 30 47429.0 31 52806.0 32 59106.5 33 65407.0 34 73728.5 35 82050.0 36 90813.0 37 99576.0 38 113329.0 39 127082.0 40 142940.0 41 158798.0 42 173139.0 43 187480.0 44 199882.0 45 212284.0 46 223864.5 47 235445.0 48 228411.0 49 221377.0 50 213463.0 51 205549.0 52 190115.5 53 174682.0 54 161554.0 55 148426.0 56 138194.0 57 127962.0 58 118662.5 59 109363.0 60 98713.5 61 88064.0 62 75890.5 63 63717.0 64 53228.0 65 42739.0 66 35454.5 67 28170.0 68 23608.0 69 19046.0 70 16229.0 71 13412.0 72 10950.0 73 8488.0 74 6550.5 75 3420.0 76 2227.0 77 1654.5 78 1082.0 79 783.5 80 485.0 81 355.5 82 226.0 83 153.5 84 81.0 85 55.5 86 30.0 87 25.5 88 21.0 89 18.0 90 15.0 91 10.0 92 5.0 93 2.5 94 0.0 95 2.5 96 5.0 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2971965.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.209040552946675 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37043408552837 20.69709550505971 2 6.4007240064765405 3.611165661338321 3 3.118322381439759 2.638946475455862 4 2.047966190691585 2.310846452971306 5 1.5903100438218378 2.243056025896432 6 1.2599965032871152 2.1325975474678343 7 1.1286112411635965 2.2285928189346764 8 0.9669773387024334 2.182200236898992 9 0.8725895639665131 2.2153422956408484 >10 8.92270168719377 49.79936957563124 >50 0.28112990485093353 4.939793993124915 >100 0.034808711518239964 1.8157545969955398 >500 0.0037395240475269977 0.705989527122373 >1k 0.001447557695812971 0.6541641041775687 >5k 0.0 0.0 >10k+ 2.4125961596882848E-4 1.8250851832844215 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34737 1.1688226476422166 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18897 0.6358419429569325 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006292133319201269 0.0 2 0.0 0.0 0.0 0.03257104306410069 0.0 3 0.0 0.0 0.0 0.05706662090569707 0.0 4 0.0 0.0 0.0 0.10410620582678463 0.0 5 0.0 0.0 0.0 0.24408093635019254 0.0 6 0.0 0.0 0.0 0.39327515633595955 0.0 7 0.0 0.0 0.0 0.49519425699831593 0.0 8 0.0 0.0 0.0 0.6959705110928291 0.0 9 0.0 0.0 0.0 0.9926092669328206 0.0 10 0.0 0.0 0.0 1.4906972323025338 0.0 11 0.0 0.0 0.0 1.7073552346679721 0.0 12 0.0 0.0 0.0 1.9553729603141357 0.0 13 0.0 0.0 0.0 2.067891109081029 0.0 14 0.0 0.0 0.0 2.1245203089538403 0.0 15 0.0 0.0 0.0 2.1810822132831307 0.0 16 3.3647771760434597E-5 0.0 0.0 2.267792521109771 0.0 17 3.3647771760434597E-5 0.0 0.0 2.3555122620892237 0.0 18 3.3647771760434597E-5 0.0 0.0 2.5159111900712157 0.0 19 3.3647771760434597E-5 0.0 0.0 2.5848218266365857 0.0 20 3.3647771760434597E-5 0.0 0.0 2.658577742335458 0.0 21 3.3647771760434597E-5 0.0 0.0 2.739870758908668 0.0 22 3.3647771760434597E-5 0.0 0.0 2.8261436457024223 0.0 23 3.3647771760434597E-5 0.0 0.0 2.9243278436993707 0.0 24 3.3647771760434597E-5 0.0 0.0 3.0057554513596223 0.0 25 3.3647771760434597E-5 0.0 0.0 3.080991869015954 0.0 26 3.3647771760434597E-5 0.0 0.0 3.155387092378275 0.0 27 3.3647771760434597E-5 0.0 0.0 3.237891428734861 0.0 28 3.3647771760434597E-5 0.0 0.0 3.3206312994937695 0.0 29 3.3647771760434597E-5 0.0 0.0 3.4121868864539118 0.0 30 3.3647771760434597E-5 0.0 0.0 3.5351021966947793 0.0 31 3.3647771760434597E-5 0.0 0.0 3.6318395405060286 0.0 32 6.729554352086919E-5 0.0 0.0 3.7350372564952816 0.0 33 6.729554352086919E-5 0.0 0.0 3.842239057324026 0.0 34 6.729554352086919E-5 0.0 0.0 3.949037084891646 0.0 35 6.729554352086919E-5 0.0 0.0 4.0710775530667425 0.0 36 6.729554352086919E-5 0.0 0.0 4.180432811288155 0.0 37 6.729554352086919E-5 0.0 0.0 4.295575486252361 0.0 38 6.729554352086919E-5 0.0 0.0 4.4301665732941 0.0 39 6.729554352086919E-5 0.0 0.0 4.581076829639649 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATCG 30 2.1666692E-6 44.999996 8 CGTTTTT 33880 0.0 43.797966 1 GTTTTTA 23040 0.0 42.67578 2 GAGCGAT 3135 0.0 39.976078 7 CGGTCTA 440 0.0 39.375 31 TATTTTT 26295 0.0 38.73645 7 TTATTTT 26410 0.0 38.601856 6 CGACGGT 460 0.0 38.152172 28 ACGGTCT 450 0.0 38.0 30 TTTTATT 26880 0.0 37.9269 4 TAATGCG 95 0.0 37.894737 1 CCGCTCG 180 0.0 37.5 19 TTTATTT 27225 0.0 37.429752 5 GTTGCGA 200 0.0 37.125 2 CACGACG 470 0.0 36.861702 26 TTTTTAT 27665 0.0 36.769386 3 TTAGCGA 340 0.0 36.397057 2 TCGCCGT 130 0.0 36.346153 25 AGCGATA 620 0.0 36.29032 8 TCACGAC 485 0.0 36.18557 25 >>END_MODULE