FastQCFastQC Report
Thu 26 May 2016
SRR1546885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546885_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences611320
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73611.204115684093437No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.6531767323169535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA551.8189894E-1245.02
GCGTTAG253.8886563E-545.01
TGTTCGG253.8886563E-545.014
TCGTAGA207.030693E-445.02
ATACGAT207.030693E-445.010
ATTGCGA406.8066583E-945.02
TATGCGA302.163828E-644.9999962
TCGTAAG302.163828E-644.9999961
CGTTTTT59400.043.5984841
AGCGATA1100.042.9545448
GTTTTTA42950.042.537832
TGCGCGA650.041.5384642
TGTGCGA556.002665E-1140.9090922
ATACGAG501.0804797E-940.53
GCACGAG850.039.7058833
CGTAAGA403.4546792E-739.3752
TAAGCGA403.4546792E-739.3752
TTATTTT48900.039.156446
TATTTTT48900.038.9723937
GAGCGAT5150.038.8834957
Produced by FastQC (version 0.11.5)