##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546885_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	611320
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632325132500164	31.0	31.0	34.0	30.0	34.0
2	31.96863835634365	31.0	31.0	34.0	30.0	34.0
3	31.908565072302558	31.0	31.0	34.0	30.0	34.0
4	35.73431917817182	37.0	35.0	37.0	35.0	37.0
5	35.74937839429431	37.0	35.0	37.0	35.0	37.0
6	35.7959219394098	37.0	35.0	37.0	35.0	37.0
7	36.00867139959432	37.0	35.0	37.0	35.0	37.0
8	13.28379408493097	0.0	0.0	35.0	0.0	37.0
9	24.86757181181705	18.0	17.0	38.0	17.0	39.0
10	32.532902571484655	32.0	32.0	37.0	27.0	39.0
11	35.47323333115226	37.0	34.0	37.0	32.0	39.0
12	36.6271461754891	37.0	35.0	39.0	33.0	39.0
13	36.9290780605902	39.0	37.0	39.0	33.0	39.0
14	38.13627069292678	40.0	37.0	41.0	33.0	41.0
15	38.376671792187395	40.0	38.0	41.0	34.0	41.0
16	38.43888470849964	40.0	38.0	41.0	34.0	41.0
17	38.3926650526729	40.0	38.0	41.0	34.0	41.0
18	38.30544559314271	40.0	37.0	41.0	33.0	41.0
19	38.24227409540012	40.0	37.0	41.0	34.0	41.0
20	38.160968069096384	40.0	37.0	41.0	34.0	41.0
21	38.10499083949486	40.0	37.0	41.0	33.0	41.0
22	38.04939148073022	40.0	36.0	41.0	33.0	41.0
23	37.87204900870248	39.0	36.0	41.0	33.0	41.0
24	37.624838055355625	39.0	35.0	41.0	33.0	41.0
25	37.49586141464372	39.0	35.0	41.0	33.0	41.0
26	37.412360138716224	39.0	35.0	41.0	33.0	41.0
27	37.3647974874043	39.0	35.0	41.0	33.0	41.0
28	37.21551724137931	39.0	35.0	41.0	33.0	41.0
29	37.14776221945953	39.0	35.0	41.0	32.0	41.0
30	36.998020676568736	39.0	35.0	41.0	32.0	41.0
31	36.87127036576588	39.0	35.0	41.0	31.0	41.0
32	36.59207943466597	39.0	35.0	41.0	31.0	41.0
33	36.283332788065174	39.0	35.0	41.0	30.0	41.0
34	35.93744683635412	39.0	35.0	41.0	27.0	41.0
35	35.67578355035006	39.0	35.0	41.0	25.0	41.0
36	35.49906104822352	39.0	35.0	41.0	24.0	41.0
37	35.4154174573055	39.0	35.0	41.0	24.0	41.0
38	35.296116600143954	39.0	35.0	41.0	23.0	41.0
39	35.18117679774913	39.0	35.0	41.0	23.0	41.0
40	35.056934175227376	39.0	35.0	40.0	23.0	41.0
41	34.95377052934633	38.0	35.0	40.0	22.0	41.0
42	34.86952823398548	38.0	34.0	40.0	22.0	41.0
43	34.76100732840411	38.0	34.0	40.0	21.0	41.0
44	34.557629392135055	38.0	34.0	40.0	21.0	41.0
45	34.47109369888111	38.0	34.0	40.0	20.0	41.0
46	34.42059968592554	38.0	34.0	40.0	20.0	41.0
47	34.35488287639861	38.0	34.0	40.0	20.0	41.0
48	34.263356343649804	38.0	34.0	40.0	20.0	41.0
49	34.163348164627365	37.0	33.0	40.0	20.0	41.0
50	34.04985932081398	37.0	33.0	40.0	20.0	41.0
51	33.69881077013675	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	6.0
15	22.0
16	42.0
17	118.0
18	273.0
19	567.0
20	962.0
21	1735.0
22	2668.0
23	4269.0
24	6619.0
25	10027.0
26	12790.0
27	13300.0
28	12748.0
29	12501.0
30	13697.0
31	16825.0
32	21511.0
33	28440.0
34	41528.0
35	60293.0
36	66920.0
37	105530.0
38	123921.0
39	53984.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.515474710462605	20.756559575999475	24.24344042400052	20.484525289537395
2	38.62118039651901	22.73817313354708	25.751161421186943	12.889485048746973
3	28.629522999411112	23.850193024929663	34.73107374206635	12.789210233592883
4	25.837531898187528	24.94798141726101	34.135804488647516	15.078682195903944
5	22.595858143034743	28.962899954197475	33.033763004645685	15.407478898122095
6	20.249787345416475	37.80524111758163	31.558430936334492	10.38654060066741
7	77.95688019367925	4.710299025060525	14.800759013282732	2.5320617679774915
8	29.202708892233197	63.84414054832166	5.412386311588039	1.5407642478570962
9	71.96558267355886	6.234050906235686	16.816070143296475	4.984296276908983
10	38.35928809788654	25.548975986390104	22.13259831184977	13.959137603873584
11	32.090558136491524	22.81701890989989	29.0718445331414	16.020578420467185
12	28.200124321141136	20.954328338676962	33.47395799254073	17.37158934764117
13	23.13092979127135	25.16603415559772	34.18455146240921	17.518484590721716
14	18.25705031734607	28.93901720866322	34.94143819930642	17.86249427468429
15	17.5371327618923	22.819799777530587	41.8762677484787	17.76679971209841
16	20.67984034548191	20.993260485506774	39.28875220833606	19.03814696067526
17	20.518059281554667	21.0506772230583	33.74648302034941	24.684780475037623
18	23.163318720146567	22.397598639010667	34.41650853889943	20.022574101943334
19	25.309657789700974	24.22642805731859	30.776843551658708	19.68707060132173
20	26.542890793692337	23.5079827259046	30.04841981286397	19.900706667539094
21	24.46361970817248	24.864882549237716	31.902604200745927	18.76889354184388
22	22.604200745926846	22.496728391022707	31.9106196427403	22.98845122031015
23	21.490872210953345	25.92570176012563	31.01239939802395	21.571026630897077
24	21.261532421644965	23.87980108617418	34.71389779493555	20.144768697245304
25	21.87757639206962	24.11306680625532	32.924654845252896	21.08470195642217
26	20.028299417653603	25.736112019891383	31.816233723745334	22.419354838709676
27	21.357717725577437	23.983183929856704	33.403454819080025	21.255643525485834
28	19.507132107570506	24.675129228554603	35.47650984754302	20.34122881633187
29	20.58185565661192	24.917555453772163	34.03487535169796	20.46571353791795
30	21.51949878950468	24.060721062618594	33.83318065824773	20.586599489629
31	25.08457109206308	22.918929529542627	30.870247987960482	21.126251390433815
32	25.17208663220572	23.884381338742394	31.05165870575149	19.8918733233004
33	23.845776352810315	24.32866583785906	30.621605705686054	21.20395210364457
34	20.856016488909248	25.602139632271154	32.22780213308905	21.31404174573055
35	21.278054046980305	25.301805928155467	32.15893476411699	21.261205260747236
36	24.12173657004515	26.638585356278217	29.000687037885232	20.2389910357914
37	21.623699535431527	27.725904599882224	29.05843093633449	21.591964928351764
38	21.699764444153637	28.67745207092848	27.50932408558529	22.11345939933259
39	21.889190603939017	26.326473859844274	28.12585879735654	23.65847673886017
40	23.444513511745075	24.781129359418962	29.50844075116142	22.26591637767454
41	20.48436170908853	24.913302362101682	29.71945953019695	24.882876398612837
42	22.368644899561605	26.418406072106258	28.097722960151806	23.11522606818033
43	22.510469148727346	26.04217103971733	28.310868285022572	23.136491526532748
44	22.236471896878886	27.047372897991234	29.211705816920762	21.50444938820912
45	21.46289995419747	27.47693515671007	28.238565726624355	22.8215991624681
46	22.89766407119021	27.004187659490935	28.610384086893937	21.487764182424918
47	21.994863573905647	26.496761107112476	29.838382516521627	21.66999280246025
48	22.17300268271936	25.49106850749198	29.895308512726558	22.440620297062093
49	21.700091605051362	24.893181966891316	30.47307465811686	22.933651769940457
50	20.11908656677354	26.877739972518484	31.18530393247399	21.817869528233985
51	20.840803507164825	26.84764116992737	29.187986651835374	23.123568671072434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	510.0
1	930.0
2	1350.0
3	9871.0
4	18392.0
5	12438.0
6	6484.0
7	6361.5
8	6239.0
9	6160.0
10	6081.0
11	5950.5
12	5820.0
13	5593.0
14	5366.0
15	5104.0
16	4842.0
17	4535.0
18	4228.0
19	4051.5
20	3875.0
21	3798.5
22	3722.0
23	3781.5
24	3841.0
25	4476.0
26	5510.5
27	5910.0
28	6720.5
29	7531.0
30	8793.0
31	10055.0
32	10758.5
33	11462.0
34	12432.5
35	13403.0
36	15362.0
37	17321.0
38	20557.5
39	23794.0
40	27581.0
41	31368.0
42	35152.5
43	38937.0
44	41826.5
45	44716.0
46	44410.5
47	44105.0
48	43106.0
49	42107.0
50	40403.0
51	38699.0
52	36943.5
53	35188.0
54	34082.5
55	32977.0
56	31737.0
57	30497.0
58	28865.0
59	27233.0
60	25074.0
61	22915.0
62	20735.5
63	18556.0
64	15936.0
65	13316.0
66	11197.5
67	9079.0
68	7712.5
69	6346.0
70	5322.0
71	4298.0
72	3507.0
73	2716.0
74	2080.5
75	1085.5
76	726.0
77	554.0
78	382.0
79	286.0
80	190.0
81	143.5
82	97.0
83	75.5
84	54.0
85	33.5
86	13.0
87	12.0
88	11.0
89	8.0
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	611320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.22181928003315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47115571915461	30.27919074107463
2	9.785502319860354	8.654654303064193
3	5.762108281822769	7.644327333322465
4	4.173397017198197	7.382208347134724
5	2.982809564157152	6.5952632746456015
6	2.2104678837787906	5.86505467684698
7	1.6711662051996072	5.173140693926507
8	1.27519731862431	4.5113236296469665
9	0.925698660159485	3.684247097160741
>10	2.6949195275212103	15.954830342778406
>50	0.029735940082982713	0.9237715813993342
>100	0.015983066352973675	1.1956430644389697
>500	0.0011150976525330263	0.27006345269084464
>1k	3.7169921751100874E-4	0.6563380198384314
>5k	3.7169921751100874E-4	1.209943442031228
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7361	1.204115684093437	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3993	0.6531767323169535	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6358044886475169E-4	0.0	0.0	0.0098148269318851	0.0
2	1.6358044886475169E-4	0.0	0.0	0.04940129555715501	0.0
3	1.6358044886475169E-4	0.0	0.0	0.08833344238696592	0.0
4	1.6358044886475169E-4	0.0	0.0	0.15049401295557155	0.0
5	1.6358044886475169E-4	0.0	0.0	0.3144016227180527	0.0
6	1.6358044886475169E-4	0.0	0.0	0.5394883203559511	0.0
7	1.6358044886475169E-4	0.0	0.0	0.6875286265785513	0.0
8	1.6358044886475169E-4	0.0	0.0	0.9827913367794281	0.0
9	1.6358044886475169E-4	0.0	0.0	1.4197147156971799	0.0
10	1.6358044886475169E-4	0.0	0.0	2.07518157429824	0.0
11	1.6358044886475169E-4	0.0	0.0	2.3686448995616045	0.0
12	1.6358044886475169E-4	0.0	0.0	2.6886082575410586	0.0
13	1.6358044886475169E-4	0.0	0.0	2.836812144212524	0.0
14	1.6358044886475169E-4	0.0	0.0	2.9251455865994895	0.0
15	1.6358044886475169E-4	0.0	0.0	3.005300006543218	0.0
16	1.6358044886475169E-4	0.0	0.0	3.1090100111234706	0.0
17	1.6358044886475169E-4	0.0	0.0	3.2197539750049073	0.0
18	1.6358044886475169E-4	0.0	0.0	3.4062356867107244	0.0
19	1.6358044886475169E-4	0.0	0.0	3.496695674932932	0.0
20	1.6358044886475169E-4	0.0	0.0	3.5860106000130862	0.0
21	1.6358044886475169E-4	0.0	0.0	3.6823594843944254	0.0
22	1.6358044886475169E-4	0.0	0.0	3.7947392527645096	0.0
23	1.6358044886475169E-4	0.0	0.0	3.9094091474187005	0.0
24	1.6358044886475169E-4	0.0	0.0	4.006412353595498	0.0
25	1.6358044886475169E-4	0.0	0.0	4.0872210953346855	0.0
26	1.6358044886475169E-4	0.0	0.0	4.180952692534189	0.0
27	1.6358044886475169E-4	0.0	0.0	4.280900346790552	0.0
28	1.6358044886475169E-4	0.0	0.0	4.369397369626382	0.0
29	1.6358044886475169E-4	0.0	0.0	4.474906759144147	0.0
30	1.6358044886475169E-4	0.0	0.0	4.617548910554211	0.0
31	1.6358044886475169E-4	0.0	0.0	4.735817575083426	0.0
32	1.6358044886475169E-4	0.0	0.0	4.872570830334358	0.0
33	1.6358044886475169E-4	0.0	0.0	5.002780867630701	0.0
34	1.6358044886475169E-4	0.0	0.0	5.129555715500883	0.0
35	1.6358044886475169E-4	0.0	0.0	5.26369168356998	0.0
36	1.6358044886475169E-4	0.0	0.0	5.385886278871949	0.0
37	1.6358044886475169E-4	0.0	0.0	5.512988287639861	0.0
38	1.6358044886475169E-4	0.0	0.0	5.6665903291238635	0.0
39	1.6358044886475169E-4	0.0	0.0	5.880553556238958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGA	55	1.8189894E-12	45.0	2
GCGTTAG	25	3.8886563E-5	45.0	1
TGTTCGG	25	3.8886563E-5	45.0	14
TCGTAGA	20	7.030693E-4	45.0	2
ATACGAT	20	7.030693E-4	45.0	10
ATTGCGA	40	6.8066583E-9	45.0	2
TATGCGA	30	2.163828E-6	44.999996	2
TCGTAAG	30	2.163828E-6	44.999996	1
CGTTTTT	5940	0.0	43.598484	1
AGCGATA	110	0.0	42.954544	8
GTTTTTA	4295	0.0	42.53783	2
TGCGCGA	65	0.0	41.538464	2
TGTGCGA	55	6.002665E-11	40.909092	2
ATACGAG	50	1.0804797E-9	40.5	3
GCACGAG	85	0.0	39.705883	3
CGTAAGA	40	3.4546792E-7	39.375	2
TAAGCGA	40	3.4546792E-7	39.375	2
TTATTTT	4890	0.0	39.15644	6
TATTTTT	4890	0.0	38.972393	7
GAGCGAT	515	0.0	38.883495	7
>>END_MODULE