FastQCFastQC Report
Thu 26 May 2016
SRR1546884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546884_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2577074
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149550.580309296512246No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83720.3248645556937829No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30990.12025265863533603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128100.043.17331
TATACGA1100.040.9090922
CGGTCTA3500.040.531
TCACGAC3600.040.025
AGGGGAA186800.039.6520358
AGCGATA3750.039.68
GTTTTTA96050.039.5887572
GAGGGGA186000.039.254037
ATATACG1150.039.1304361
GCGCGAC10150.039.014789
CACGACG3650.038.83561726
TATACGT356.25131E-638.5714316
GAGCGAT23450.038.475487
TATAGCG2050.038.4146351
ACGACGG3700.037.70270527
ATACGGT301.1403153E-437.50000421
TTCGCTA301.1403153E-437.50000445
CGACGGT3850.037.402628
CGTTAGA2300.037.1739162
ACGTTAC850.037.05882345
Produced by FastQC (version 0.11.5)