##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546884_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2577074
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72262728970918	31.0	31.0	34.0	30.0	34.0
2	32.088646270925864	33.0	31.0	34.0	30.0	34.0
3	32.0847391266219	33.0	31.0	34.0	30.0	34.0
4	35.83877684536804	37.0	35.0	37.0	35.0	37.0
5	35.83405521145299	37.0	35.0	37.0	35.0	37.0
6	35.877404374108	37.0	35.0	37.0	35.0	37.0
7	36.09116657108022	37.0	35.0	37.0	35.0	37.0
8	13.241937173709408	0.0	0.0	35.0	0.0	37.0
9	24.905889004351447	18.0	17.0	39.0	17.0	39.0
10	32.62773207133361	32.0	32.0	37.0	27.0	39.0
11	35.545514020940026	37.0	35.0	37.0	32.0	39.0
12	36.62571039869247	37.0	35.0	39.0	33.0	39.0
13	36.86788388691982	39.0	35.0	39.0	33.0	39.0
14	38.033396790313354	40.0	37.0	41.0	33.0	41.0
15	38.32185183661781	40.0	37.0	41.0	34.0	41.0
16	38.411739049790576	40.0	37.0	41.0	34.0	41.0
17	38.40162020958653	40.0	37.0	41.0	34.0	41.0
18	38.33617854978165	40.0	37.0	41.0	34.0	41.0
19	38.3135664711219	40.0	37.0	41.0	34.0	41.0
20	38.26607384964498	40.0	37.0	41.0	34.0	41.0
21	38.19788605216614	40.0	37.0	41.0	34.0	41.0
22	38.139542752749826	40.0	37.0	41.0	34.0	41.0
23	38.02060127105391	40.0	36.0	41.0	34.0	41.0
24	37.895284341854364	40.0	36.0	41.0	33.0	41.0
25	37.786575007159286	40.0	35.0	41.0	33.0	41.0
26	37.705517187321746	40.0	35.0	41.0	33.0	41.0
27	37.6564169286563	40.0	35.0	41.0	33.0	41.0
28	37.556752735854694	40.0	35.0	41.0	33.0	41.0
29	37.54348342344846	40.0	35.0	41.0	33.0	41.0
30	37.45603812696104	40.0	35.0	41.0	33.0	41.0
31	37.367274668868646	40.0	35.0	41.0	33.0	41.0
32	37.198924439111956	40.0	35.0	41.0	32.0	41.0
33	37.023158434720926	40.0	35.0	41.0	31.0	41.0
34	36.82211841801982	40.0	35.0	41.0	31.0	41.0
35	36.67362869673126	40.0	35.0	41.0	31.0	41.0
36	36.54956978340552	40.0	35.0	41.0	30.0	41.0
37	36.48875313630885	40.0	35.0	41.0	30.0	41.0
38	36.38874359059926	39.0	35.0	41.0	30.0	41.0
39	36.315733269591796	39.0	35.0	41.0	30.0	41.0
40	36.20772822200682	39.0	35.0	41.0	30.0	41.0
41	36.14089816590443	39.0	35.0	41.0	29.0	41.0
42	36.08858069267704	39.0	35.0	41.0	29.0	41.0
43	35.99298118719137	39.0	35.0	41.0	29.0	41.0
44	35.81040901425415	39.0	35.0	40.0	28.0	41.0
45	35.707944746639015	39.0	35.0	40.0	28.0	41.0
46	35.682947016655326	38.0	35.0	40.0	28.0	41.0
47	35.6365401226352	38.0	35.0	40.0	28.0	41.0
48	35.560974190108624	38.0	35.0	40.0	27.0	41.0
49	35.45274990163263	38.0	35.0	40.0	27.0	41.0
50	35.35018901281065	38.0	35.0	40.0	27.0	41.0
51	34.97636777213227	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	11.0
12	10.0
13	20.0
14	25.0
15	47.0
16	131.0
17	303.0
18	730.0
19	1504.0
20	2949.0
21	4813.0
22	7952.0
23	12371.0
24	18790.0
25	27591.0
26	34791.0
27	38003.0
28	38886.0
29	42648.0
30	50555.0
31	64262.0
32	84361.0
33	116153.0
34	176172.0
35	265612.0
36	276607.0
37	463577.0
38	597902.0
39	250248.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.50276321130088	22.61576501101637	27.985187852580097	15.896283925102656
2	35.27803237314877	23.385436351459056	28.134116443687684	13.202414831704484
3	29.907134991079033	24.36569535837931	31.891711297386106	13.835458353155555
4	26.63710859680397	26.5586087167074	31.173959304234184	15.63032338225445
5	23.228514198660964	30.86384015360056	30.1699912381251	15.737654409613382
6	21.246886973365918	38.91397763510089	29.090045532258678	10.74908985927451
7	83.68762402631822	4.9038172749404945	9.1186749002939	2.2898837984473865
8	31.62641041739585	63.84135651517961	3.3394850128479043	1.1927480545766245
9	79.76072864031067	5.43845461946378	11.085285094646098	3.7155316455794436
10	44.45797831183738	25.466866686792848	16.909409663828047	13.165745337541724
11	37.81754035778561	23.384893099693684	23.708942777739406	15.088623764781298
12	33.429734652555574	22.38045938921428	28.452578389289556	15.73722756894059
13	24.126160133546808	30.381510193343303	28.930833961306508	16.56149571180339
14	18.42174497123482	33.79708925704112	31.256378357780957	16.524787413943102
15	17.71641016129145	24.196084396489972	41.555151307257766	16.532354134960812
16	20.538409063922884	21.429807603506923	39.3078351649972	18.723948167572992
17	20.663706203236696	20.959041145112636	30.25625185772702	28.12100079392365
18	23.681741385773165	23.056458603827444	32.650789228404	20.611010781995397
19	26.929222831785193	25.088996280277552	28.009828200509567	19.97195268742768
20	28.15390632942632	24.333565896827178	26.70109589402555	20.811431879720953
21	25.434349188265454	25.513819160800193	29.593523507668003	19.458308143266358
22	23.401501082235125	23.490710782849074	29.599576884482172	23.508211250433632
23	21.146113770889	27.620976347594212	28.199423066625172	23.033486814891617
24	21.685135933232804	25.392441194936584	33.433615022308246	19.488807849522367
25	21.47210363381106	24.356537685763	32.38219003412397	21.789168646301967
26	19.3303335488232	28.582337953818943	29.441684639245903	22.645643858111953
27	20.318159276761165	27.231076794845627	31.34554925469738	21.105214673695826
28	18.708349081167245	26.8306614400673	35.07990069357729	19.381088785188165
29	19.553493613299423	25.7629388989218	34.30887122372116	20.374696264057608
30	21.518086015380234	25.714589491803498	32.590333067657355	20.17699142515892
31	26.653406149765196	24.546210159273656	28.041686036179016	20.75869765478213
32	26.839896720078666	26.11562570574225	27.950730169176364	19.093747405002727
33	25.50035427775842	25.3866594440051	28.319016062402554	20.793970215833927
34	21.04204225412231	26.61627101123212	30.191333271764798	22.15035346288077
35	20.841000297236324	25.85517528794284	30.72946294906549	22.57436146575535
36	26.157533699071116	26.20262359559718	27.859696694778652	19.780146010553054
37	22.076471222789877	28.992803466256696	28.348118835547602	20.58260647540583
38	21.734882273462077	31.135931680657986	25.013406677495485	22.115779368384455
39	21.819862371045613	28.293560836825023	26.81611005349478	23.07046673863459
40	22.68177010050934	25.741170024609307	27.537315575726577	24.039744299154776
41	20.212768434278566	25.407574636972008	27.939515900591132	26.440141028158294
42	22.320003228467634	26.860695501952993	26.379413241528958	24.439888028050415
43	22.259585871418516	26.603116557770555	26.84408751941155	24.293210051399377
44	21.286699567028343	28.87596553300371	28.04816625366598	21.789168646301967
45	20.67383396829117	30.430790889202253	26.471067575087094	22.424307567419486
46	22.74653347168145	28.43534954758769	28.117314442658614	20.700802538072246
47	22.244141999802878	27.58582019763499	28.095429157253538	22.074608645308594
48	22.714908458197165	26.119932916167716	29.001922335175472	22.163236290459647
49	21.57140229578196	25.149336029931618	30.506613314169478	22.77264836011694
50	20.531967650133446	28.81578099813975	28.73239185215481	21.919859499571995
51	20.33837600317259	29.734652555572715	27.08249743701578	22.844474004238915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1788.0
1	3695.5
2	5603.0
3	22767.0
4	39931.0
5	27362.0
6	14793.0
7	14703.0
8	14613.0
9	14849.0
10	15085.0
11	15113.0
12	15141.0
13	14337.0
14	13533.0
15	13161.0
16	12789.0
17	11869.5
18	10950.0
19	10801.0
20	10652.0
21	11030.5
22	11409.0
23	13210.0
24	15011.0
25	17243.5
26	22332.5
27	25189.0
28	28928.5
29	32668.0
30	37703.0
31	42738.0
32	47810.5
33	52883.0
34	59031.0
35	65179.0
36	74813.5
37	84448.0
38	104926.5
39	125405.0
40	150706.0
41	176007.0
42	189646.5
43	203286.0
44	207652.5
45	212019.0
46	210842.5
47	209666.0
48	200895.5
49	192125.0
50	183571.5
51	175018.0
52	164513.5
53	154009.0
54	146597.0
55	139185.0
56	131863.5
57	124542.0
58	114203.0
59	103864.0
60	91312.5
61	78761.0
62	69377.0
63	59993.0
64	51289.5
65	42586.0
66	35621.5
67	28657.0
68	24055.0
69	19453.0
70	16014.5
71	12576.0
72	10135.0
73	7694.0
74	6071.5
75	3318.0
76	2187.0
77	1579.5
78	972.0
79	712.0
80	452.0
81	302.0
82	152.0
83	98.0
84	44.0
85	40.0
86	36.0
87	23.5
88	11.0
89	12.5
90	14.0
91	15.0
92	16.0
93	12.5
94	9.0
95	7.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2577074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.21407616065424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64138014358669	17.247315317279426
2	5.975890381456946	2.654977681228114
3	2.269527475606478	1.5124636857545897
4	1.3106763563102852	1.1646185760418186
5	0.9581597249402352	1.064231655194695
6	0.740584694302267	0.9870842881587238
7	0.6162796875806736	0.9583058741326898
8	0.5179306979088636	0.9204281575430638
9	0.46588089591124826	0.931420233320967
>10	7.7826037529616325	42.90656921285967
>50	1.5597862074896969	23.088914283310473
>100	0.15673850668523104	4.77861956491196
>500	0.003284262187054198	0.5124002789917299
>1k	9.122950519594995E-4	0.3271709422576526
>5k	1.824590103918999E-4	0.3393304173167824
>10k+	1.824590103918999E-4	0.6061498316976207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14955	0.580309296512246	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8372	0.3248645556937829	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3099	0.12025265863533603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8803697526729926E-5	0.0	0.0	0.005316106561162	0.0
2	3.8803697526729926E-5	0.0	0.0	0.01963467094852534	0.0
3	3.8803697526729926E-5	0.0	0.0	0.035039738866637124	0.0
4	3.8803697526729926E-5	0.0	0.0	0.05968008679611063	0.0
5	3.8803697526729926E-5	0.0	0.0	0.12281370267210022	0.0
6	3.8803697526729926E-5	0.0	0.0	0.21082048866272368	0.0
7	3.8803697526729926E-5	0.0	0.0	0.26627097242842074	0.0
8	3.8803697526729926E-5	0.0	0.0	0.40860293495646616	0.0
9	7.760739505345985E-5	0.0	0.0	0.5944338424119757	0.0
10	7.760739505345985E-5	0.0	0.0	0.8932223133677962	0.0
11	7.760739505345985E-5	0.0	0.0	1.090655526383798	0.0
12	7.760739505345985E-5	0.0	0.0	1.278310207623064	0.0
13	7.760739505345985E-5	0.0	0.0	1.3625530349535946	0.0
14	7.760739505345985E-5	0.0	0.0	1.3972435405424912	0.0
15	7.760739505345985E-5	0.0	0.0	1.4405096632847951	0.0
16	7.760739505345985E-5	0.0	0.0	1.5332893040712063	0.0
17	7.760739505345985E-5	0.0	0.0	1.6385249317636978	0.0
18	7.760739505345985E-5	0.0	0.0	1.8141116630721508	0.0
19	7.760739505345985E-5	0.0	0.0	1.8881491179531515	0.0
20	1.1641109258018978E-4	0.0	0.0	1.968395164438429	0.0
21	1.1641109258018978E-4	0.0	0.0	2.0719234294397446	0.0
22	1.1641109258018978E-4	0.0	0.0	2.1819707156255506	0.0
23	1.1641109258018978E-4	0.0	0.0	2.295510334588762	0.0
24	1.1641109258018978E-4	0.0	0.0	2.385069268480455	0.0
25	1.552147901069197E-4	0.0	0.0	2.461939393280907	0.0
26	1.552147901069197E-4	0.0	0.0	2.542961513716719	0.0
27	1.552147901069197E-4	0.0	0.0	2.62541937096102	0.0
28	1.9401848763364964E-4	0.0	0.0	2.7178497784696907	0.0
29	1.9401848763364964E-4	0.0	0.0	2.8178469069960737	0.0
30	1.9401848763364964E-4	0.0	0.0	2.9502063192597494	0.0
31	2.3282218516037956E-4	0.0	0.0	3.065492104611664	0.0
32	2.3282218516037956E-4	0.0	0.0	3.175772212982631	0.0
33	2.3282218516037956E-4	0.0	0.0	3.28578069547091	0.0
34	2.3282218516037956E-4	0.0	0.0	3.404636420995284	0.0
35	2.3282218516037956E-4	0.0	0.0	3.544446143184092	0.0
36	2.3282218516037956E-4	0.0	0.0	3.669277638127582	0.0
37	2.3282218516037956E-4	0.0	0.0	3.788404989534643	0.0
38	2.3282218516037956E-4	0.0	0.0	3.9365575066916976	0.0
39	2.3282218516037956E-4	0.0	0.0	4.132554982899211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12810	0.0	43.1733	1
TATACGA	110	0.0	40.909092	2
CGGTCTA	350	0.0	40.5	31
TCACGAC	360	0.0	40.0	25
AGGGGAA	18680	0.0	39.652035	8
AGCGATA	375	0.0	39.6	8
GTTTTTA	9605	0.0	39.588757	2
GAGGGGA	18600	0.0	39.25403	7
ATATACG	115	0.0	39.130436	1
GCGCGAC	1015	0.0	39.01478	9
CACGACG	365	0.0	38.835617	26
TATACGT	35	6.25131E-6	38.57143	16
GAGCGAT	2345	0.0	38.47548	7
TATAGCG	205	0.0	38.414635	1
ACGACGG	370	0.0	37.702705	27
ATACGGT	30	1.1403153E-4	37.500004	21
TTCGCTA	30	1.1403153E-4	37.500004	45
CGACGGT	385	0.0	37.4026	28
CGTTAGA	230	0.0	37.173916	2
ACGTTAC	85	0.0	37.058823	45
>>END_MODULE