FastQCFastQC Report
Thu 26 May 2016
SRR1546883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546883_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2056930
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180150.8758197896865718No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99210.4823207401321387No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23470.11410208417398744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153550.043.549331
ACGGTCT5900.043.4745730
CGGTCTA6150.041.7073231
GTTTTTA106150.041.396612
ACGACGG6150.041.34146527
CGACGGT6400.041.13281628
TCACGAC6150.040.97561325
TATCGCG501.0822987E-940.51
CACGACG6350.040.39370326
GACGGTC6700.039.62686529
GGTCTAA6550.039.50381532
GAGCGAT15400.039.448057
TACGACG403.4599907E-739.3750041
CGCATCG1050.038.5714321
CACGACC3200.037.96875427
AGGGGAA115250.037.8937078
TAGAGTA8500.037.5882385
CTATACG601.5643309E-1037.5000041
CTAACCT7200.037.515
TTATTTT123600.037.4271856
Produced by FastQC (version 0.11.5)