##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546883_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2056930
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69406883073318	31.0	31.0	34.0	30.0	34.0
2	32.050549605480015	33.0	31.0	34.0	30.0	34.0
3	32.021298245443454	33.0	31.0	34.0	30.0	34.0
4	35.810082015430766	37.0	35.0	37.0	35.0	37.0
5	35.82545638402863	37.0	35.0	37.0	35.0	37.0
6	35.87005974923794	37.0	35.0	37.0	35.0	37.0
7	36.09340084494854	37.0	35.0	37.0	35.0	37.0
8	13.27476919486808	0.0	0.0	35.0	0.0	37.0
9	24.91577204863559	18.0	17.0	39.0	17.0	39.0
10	32.556040798665975	32.0	32.0	37.0	27.0	39.0
11	35.518015197405845	37.0	34.0	37.0	32.0	39.0
12	36.673365160700655	37.0	35.0	39.0	33.0	39.0
13	36.96169193895757	39.0	37.0	39.0	33.0	39.0
14	38.16587341329068	40.0	37.0	41.0	33.0	41.0
15	38.42168960538278	40.0	38.0	41.0	34.0	41.0
16	38.47807898178353	40.0	38.0	41.0	34.0	41.0
17	38.435465961408504	40.0	38.0	41.0	34.0	41.0
18	38.36597988264064	40.0	37.0	41.0	34.0	41.0
19	38.33508286621324	40.0	37.0	41.0	34.0	41.0
20	38.2770842955278	40.0	37.0	41.0	34.0	41.0
21	38.2206044931038	40.0	37.0	41.0	34.0	41.0
22	38.16436631290321	40.0	37.0	41.0	34.0	41.0
23	38.01658636900624	40.0	37.0	41.0	34.0	41.0
24	37.831019529104054	40.0	35.0	41.0	33.0	41.0
25	37.71659706455738	40.0	35.0	41.0	33.0	41.0
26	37.65515938802001	40.0	35.0	41.0	33.0	41.0
27	37.608312387879025	40.0	35.0	41.0	33.0	41.0
28	37.49083537115993	40.0	35.0	41.0	33.0	41.0
29	37.44339185096236	40.0	35.0	41.0	33.0	41.0
30	37.31580802458032	40.0	35.0	41.0	33.0	41.0
31	37.1929613550291	40.0	35.0	41.0	32.0	41.0
32	36.97026247854813	40.0	35.0	41.0	31.0	41.0
33	36.71809930333069	40.0	35.0	41.0	31.0	41.0
34	36.43522676999217	40.0	35.0	41.0	30.0	41.0
35	36.223052801991315	40.0	35.0	41.0	29.0	41.0
36	36.08417739057722	40.0	35.0	41.0	28.0	41.0
37	36.02046836790751	39.0	35.0	41.0	28.0	41.0
38	35.91475110966343	39.0	35.0	41.0	27.0	41.0
39	35.81612889111443	39.0	35.0	41.0	27.0	41.0
40	35.71103635028902	39.0	35.0	41.0	26.0	41.0
41	35.62961306412955	39.0	35.0	41.0	26.0	41.0
42	35.56397932841662	39.0	35.0	41.0	26.0	41.0
43	35.45030360780386	39.0	35.0	40.0	25.0	41.0
44	35.27525584244481	38.0	35.0	40.0	24.0	41.0
45	35.184042723865176	38.0	35.0	40.0	24.0	41.0
46	35.153724239522006	38.0	35.0	40.0	24.0	41.0
47	35.103219360892204	38.0	34.0	40.0	24.0	41.0
48	35.01306461571371	38.0	34.0	40.0	23.0	41.0
49	34.90995804426986	38.0	34.0	40.0	24.0	41.0
50	34.79986241631946	38.0	34.0	40.0	24.0	41.0
51	34.43692250100879	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	5.0
14	24.0
15	40.0
16	104.0
17	286.0
18	586.0
19	1360.0
20	2640.0
21	4432.0
22	7116.0
23	11482.0
24	18316.0
25	27490.0
26	35437.0
27	37507.0
28	36291.0
29	37529.0
30	42643.0
31	52206.0
32	67995.0
33	91895.0
34	136844.0
35	203892.0
36	218830.0
37	366595.0
38	456590.0
39	198747.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.89342369453506	22.30693314794378	26.30974316092429	17.48989999659687
2	37.21327415128371	23.04147443034036	27.071995643993716	12.673255774382211
3	29.594103834355085	23.539012022771804	33.97996042646079	12.886923716412324
4	25.727175936954588	25.879782005221376	32.874721064887964	15.518320992936074
5	22.8426344114773	29.997277496074247	32.122094577841736	15.03799351460672
6	20.6162582100509	38.857228977165	30.24872018007419	10.277792632709911
7	82.23045995731503	4.289159086599933	11.414438021711968	2.0659429343730706
8	31.037857389410433	63.621319150384316	4.175397315416665	1.1654261447885927
9	77.59559148828595	5.338003723996441	13.277262716767222	3.789142070950397
10	40.06723612373781	25.01062262692459	21.24471907162616	13.677422177711444
11	34.3540130194027	23.327094261836816	26.548983193399874	15.769909525360609
12	30.735513605227204	22.414958214426353	30.563023535074112	16.286504645272323
13	23.57975234937504	27.85038868605154	31.272430272299008	17.29742869227441
14	18.743078276849477	31.428390854331457	32.98663542269304	16.841895446126024
15	18.570053429139545	23.815735100368023	40.642024765062494	16.972186705429937
16	21.440593505855816	21.146806162582102	39.151113552721775	18.26148677884031
17	20.921859275716724	20.98190021050789	32.42473005887415	25.671510454901238
18	23.655496297880823	23.04176612718955	33.849474702590754	19.453262872338875
19	26.11673707904498	25.022533581599767	29.478883578925874	19.38184576042938
20	27.108311901717606	24.636181104850433	28.606223838438837	19.64928315499312
21	24.621742110815635	26.16574214970855	31.05633152319233	18.156184216283492
22	22.872581954660586	23.227528403980692	31.360474104612212	22.53941553674651
23	21.149528666507855	26.431186282469504	31.068388326292094	21.350896724730546
24	22.160112400519218	24.657134661850428	34.02755562901994	19.155197308610404
25	21.513614950435844	24.141220167920153	33.10496711118026	21.24019777046375
26	19.464055655758823	27.658549391568986	30.524325086415192	22.353069866256995
27	20.259269882786484	26.246347712367463	32.349180574934486	21.145201829911567
28	18.681238544821653	26.222525803017117	35.397072335956985	19.699163316204245
29	20.446344795398968	25.449091607395484	34.41021327901289	19.694350318192647
30	22.239502559639853	24.839347960309784	33.76697311041212	19.154176369638247
31	26.51597283330011	24.05478066827748	29.420106663814522	20.009139834607886
32	26.987743870719953	25.245389974379297	29.15908659993291	18.60777955496784
33	25.803065733884967	24.77036165547685	29.146592251559362	20.279980359078824
34	21.416820212647004	26.701929574657377	31.27943099667952	20.6018192160161
35	21.505155741809396	25.95902631591741	31.081757765213208	21.45406017705999
36	25.684004803274785	26.834943337887047	28.312922656580437	19.168129202257735
37	22.40596422824306	29.1200964544248	28.536411059199875	19.937528258132264
38	22.241495821442637	30.57435109605091	26.29642233814471	20.887730744361743
39	22.346360838725673	27.69350439733	27.348913186156064	22.611221577788257
40	23.103071081660534	25.440875479476695	28.277627337828704	23.178426101034066
41	20.365739232740054	25.390509156850253	28.61482889548988	25.628922714919806
42	22.38170477361894	27.12061178552503	26.7983839994555	23.699299441400534
43	21.794664864628356	26.3111530290287	27.57740905135323	24.316773054989717
44	21.25458814835702	27.790639448109562	28.91260276236916	22.042169641164257
45	21.089633580141278	29.1865547198981	27.565741177385718	22.158070522574906
46	22.99319860179977	28.176505763443576	28.552162689056022	20.27813294570063
47	22.206638047964685	27.052646419664256	28.468299844914508	22.272415687456547
48	21.801325276018144	26.36341538117486	29.887016087081232	21.948243255725767
49	22.27649944334518	24.913195879295845	30.593068310540467	22.21723636681851
50	20.70556606204392	27.46102200852727	29.540237149538388	22.293174779890418
51	20.741007229220244	28.402279124714987	28.656249848074555	22.200463797990206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1377.0
1	2601.5
2	3826.0
3	25511.0
4	47196.0
5	31999.5
6	16803.0
7	16521.0
8	16239.0
9	16391.5
10	16544.0
11	16099.0
12	15654.0
13	15046.0
14	14438.0
15	13530.5
16	12623.0
17	12100.0
18	11577.0
19	11247.5
20	10918.0
21	11407.5
22	11897.0
23	12363.5
24	12830.0
25	14357.5
26	17754.5
27	19624.0
28	23014.0
29	26404.0
30	31083.0
31	35762.0
32	39530.0
33	43298.0
34	47828.5
35	52359.0
36	59943.5
37	67528.0
38	80089.5
39	92651.0
40	108646.5
41	124642.0
42	133055.5
43	141469.0
44	145637.0
45	149805.0
46	154081.5
47	158358.0
48	158475.0
49	158592.0
50	152789.5
51	146987.0
52	135583.5
53	124180.0
54	115912.0
55	107644.0
56	102094.5
57	96545.0
58	90465.0
59	84385.0
60	76650.0
61	68915.0
62	60046.0
63	51177.0
64	42568.0
65	33959.0
66	28230.0
67	22501.0
68	19414.5
69	16328.0
70	14015.5
71	11703.0
72	9377.0
73	7051.0
74	5309.0
75	2734.5
76	1902.0
77	1406.0
78	910.0
79	672.0
80	434.0
81	329.5
82	225.0
83	167.5
84	110.0
85	82.5
86	55.0
87	42.0
88	29.0
89	23.0
90	17.0
91	9.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2056930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.587433853697163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66195129333586	19.58482257545829
2	6.287880918095149	3.3435723597955866
3	2.917429190098225	2.327008668437455
4	1.9454331846645443	2.0689630445622393
5	1.4391826607478413	1.9132086898010554
6	1.163722850988298	1.8564242594812355
7	0.9963734115564122	1.8543708521337083
8	0.8897610829020065	1.8925171149800843
9	0.8028559332033199	1.9211291116282418
>10	9.473671424704843	52.919023924787275
>50	0.37902981258492147	6.25257391439632
>100	0.03924545969594976	1.9279395498264862
>500	0.002500894805237264	0.47690377419347607
>1k	5.771295704393686E-4	0.2326721652516219
>5k	1.9237652347978953E-4	0.5074391188088241
>10k+	1.9237652347978953E-4	0.9214308764581156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18015	0.8758197896865718	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9921	0.4823207401321387	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2347	0.11410208417398744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005396391709975546	0.0
2	0.0	0.0	0.0	0.018814446772617444	0.0
3	0.0	0.0	0.0	0.03665657071460866	0.0
4	0.0	0.0	0.0	0.05853383440369871	0.0
5	0.0	0.0	0.0	0.11492855858001974	0.0
6	0.0	0.0	0.0	0.22893341047094456	0.0
7	0.0	0.0	0.0	0.304920439684384	0.0
8	0.0	0.0	0.0	0.4785286810926964	0.0
9	0.0	0.0	0.0	0.7519944772063221	0.0
10	0.0	0.0	0.0	1.1240051922039156	0.0
11	0.0	0.0	0.0	1.329019461041455	0.0
12	0.0	0.0	0.0	1.5197892004103202	0.0
13	0.0	0.0	0.0	1.6099235268093712	0.0
14	0.0	0.0	0.0	1.652365418366206	0.0
15	0.0	0.0	0.0	1.6968491878673557	0.0
16	0.0	0.0	0.0	1.7721555910993567	0.0
17	0.0	0.0	0.0	1.8562615159485252	0.0
18	0.0	0.0	0.0	1.9971997102477965	0.0
19	0.0	0.0	0.0	2.05665725134059	0.0
20	0.0	0.0	0.0	2.132109502997185	0.0
21	0.0	0.0	0.0	2.218792083347513	0.0
22	0.0	0.0	0.0	2.3098015002941277	0.0
23	0.0	0.0	0.0	2.4120412459344753	0.0
24	0.0	0.0	0.0	2.491868950328888	0.0
25	0.0	0.0	0.0	2.5639666882198227	0.0
26	0.0	0.0	0.0	2.636501971384539	0.0
27	0.0	0.0	0.0	2.71180837461654	0.0
28	0.0	0.0	0.0	2.7975186321362417	0.0
29	0.0	0.0	0.0	2.889548988055014	0.0
30	0.0	0.0	0.0	3.0097767060619467	0.0
31	4.8616141531311224E-5	0.0	0.0	3.1089536347858218	0.0
32	4.8616141531311224E-5	0.0	0.0	3.211485077275357	0.0
33	4.8616141531311224E-5	0.0	0.0	3.316447326841458	0.0
34	4.8616141531311224E-5	0.0	0.0	3.429431239760225	0.0
35	4.8616141531311224E-5	0.0	0.0	3.5516036034284104	0.0
36	4.8616141531311224E-5	0.0	0.0	3.6636151935165513	0.0
37	4.8616141531311224E-5	0.0	0.0	3.7802939331916985	0.0
38	4.8616141531311224E-5	0.0	0.0	3.9337264758645167	0.0
39	4.8616141531311224E-5	0.0	0.0	4.13222618173686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15355	0.0	43.54933	1
ACGGTCT	590	0.0	43.47457	30
CGGTCTA	615	0.0	41.70732	31
GTTTTTA	10615	0.0	41.39661	2
ACGACGG	615	0.0	41.341465	27
CGACGGT	640	0.0	41.132816	28
TCACGAC	615	0.0	40.975613	25
TATCGCG	50	1.0822987E-9	40.5	1
CACGACG	635	0.0	40.393703	26
GACGGTC	670	0.0	39.626865	29
GGTCTAA	655	0.0	39.503815	32
GAGCGAT	1540	0.0	39.44805	7
TACGACG	40	3.4599907E-7	39.375004	1
CGCATCG	105	0.0	38.57143	21
CACGACC	320	0.0	37.968754	27
AGGGGAA	11525	0.0	37.893707	8
TAGAGTA	850	0.0	37.588238	5
CTATACG	60	1.5643309E-10	37.500004	1
CTAACCT	720	0.0	37.5	15
TTATTTT	12360	0.0	37.427185	6
>>END_MODULE