FastQCFastQC Report
Thu 26 May 2016
SRR1546882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546882_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1257591
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51240.4074456639718319No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.2705172031288392No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.1431307953062641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG750.045.0000041
CCGTAGG302.1656651E-645.0000041
CTACGCG351.2120472E-745.0000041
TACGACA302.1656651E-645.00000423
GTCACGA700.045.0000042
GTTCACG551.8189894E-1245.0000041
CGTGTAA302.1656651E-645.00000426
GCGTGAT302.1656651E-645.00000416
GCTACGA600.045.0000042
CGCGTCA302.1656651E-645.00000439
ATTGCGA1250.045.0000042
TATCGTA302.1656651E-645.00000426
TAGTCTA302.1656651E-645.0000042
GTACCGA207.0335396E-445.02
CCTAGGT207.0335396E-445.039
AAGACGC207.0335396E-445.038
ATTCGAA207.0335396E-445.03
TAACGGC207.0335396E-445.017
TATGCGT207.0335396E-445.020
GTAGCGC207.0335396E-445.034