##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546882_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1257591
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75380946587563	31.0	31.0	34.0	30.0	34.0
2	32.09138265143437	33.0	31.0	34.0	30.0	34.0
3	32.11391939032642	33.0	31.0	34.0	30.0	34.0
4	35.83573514759568	37.0	35.0	37.0	35.0	37.0
5	35.79764406710926	37.0	35.0	37.0	35.0	37.0
6	35.844311862918865	37.0	35.0	37.0	35.0	37.0
7	36.078270280242144	37.0	35.0	37.0	35.0	37.0
8	13.284755536577473	0.0	0.0	36.0	0.0	37.0
9	24.97634286504913	18.0	17.0	39.0	17.0	39.0
10	32.76302549875118	32.0	32.0	38.0	27.0	39.0
11	35.65917933572998	37.0	35.0	37.0	32.0	39.0
12	36.41872118995762	37.0	35.0	39.0	33.0	39.0
13	36.374204331933036	38.0	35.0	39.0	32.0	39.0
14	37.475914665419836	39.0	36.0	41.0	33.0	41.0
15	37.910655372056574	40.0	36.0	41.0	33.0	41.0
16	38.10143520429138	40.0	36.0	41.0	34.0	41.0
17	38.115211543339605	40.0	36.0	41.0	34.0	41.0
18	38.0707861299898	40.0	36.0	41.0	34.0	41.0
19	38.0259456373336	40.0	36.0	41.0	34.0	41.0
20	37.990689341765325	40.0	36.0	41.0	34.0	41.0
21	37.88293014183466	40.0	35.0	41.0	33.0	41.0
22	37.821817268094314	40.0	35.0	41.0	33.0	41.0
23	37.763569395773345	40.0	35.0	41.0	33.0	41.0
24	37.71128689693231	40.0	35.0	41.0	33.0	41.0
25	37.60032156718679	40.0	35.0	41.0	33.0	41.0
26	37.51730968176458	40.0	35.0	41.0	33.0	41.0
27	37.420309941785526	39.0	35.0	41.0	33.0	41.0
28	37.4090519095636	39.0	35.0	41.0	33.0	41.0
29	37.478957785162265	40.0	35.0	41.0	33.0	41.0
30	37.43461825028964	40.0	35.0	41.0	33.0	41.0
31	37.31347393548459	40.0	35.0	41.0	33.0	41.0
32	37.14029919107246	39.0	35.0	41.0	32.0	41.0
33	37.035717494797595	40.0	35.0	41.0	32.0	41.0
34	36.938005281526344	40.0	35.0	41.0	32.0	41.0
35	36.845230285522085	40.0	35.0	41.0	32.0	41.0
36	36.73002192286681	39.0	35.0	41.0	31.0	41.0
37	36.614958281349026	39.0	35.0	41.0	31.0	41.0
38	36.556056778396155	39.0	35.0	41.0	31.0	41.0
39	36.54067816961158	39.0	35.0	41.0	31.0	41.0
40	36.395115741127285	39.0	35.0	41.0	30.0	41.0
41	36.38751311038327	39.0	35.0	41.0	31.0	41.0
42	36.34575549602375	39.0	35.0	41.0	30.0	41.0
43	36.2226979995881	39.0	35.0	41.0	30.0	41.0
44	36.04354038793217	38.0	35.0	40.0	30.0	41.0
45	35.94290830643667	38.0	35.0	40.0	30.0	41.0
46	35.96286471515779	38.0	35.0	40.0	30.0	41.0
47	35.933971378611965	38.0	35.0	40.0	30.0	41.0
48	35.90757169858881	38.0	35.0	40.0	30.0	41.0
49	35.85329093481108	38.0	35.0	40.0	29.0	41.0
50	35.70267916993681	38.0	35.0	40.0	29.0	41.0
51	35.25917806345624	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	2.0
14	8.0
15	20.0
16	46.0
17	128.0
18	337.0
19	669.0
20	1263.0
21	2117.0
22	3356.0
23	5099.0
24	7657.0
25	10853.0
26	13763.0
27	15534.0
28	16869.0
29	19725.0
30	24432.0
31	32305.0
32	43355.0
33	61246.0
34	101306.0
35	166943.0
36	120995.0
37	207531.0
38	306070.0
39	95927.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52432627141893	22.699351378945938	30.467059640216892	14.309262709418244
2	29.518897638421393	25.73014596955608	31.394944779343998	13.356011612678525
3	28.23891074284088	24.556711999370222	32.812575789744045	14.391801468044857
4	25.63067006681823	26.827879652446622	31.636994857628594	15.904455423106558
5	23.395603181002407	31.70935542636676	30.251011656412935	14.644029736217895
6	21.058992947627647	40.63356051371233	26.945962558574287	11.361483980085735
7	85.91529360499558	5.428951065966598	6.829247346712881	1.826507982324937
8	33.110208326872566	63.60812060518881	2.2097009282032074	1.0719701397354147
9	84.74074639529067	4.418845236646891	7.634835172961639	3.205573195100792
10	60.820171263948296	18.10874918793153	11.094306495514042	9.976773052606132
11	55.54110994751076	16.608977004447393	17.015150394683168	10.834762653358682
12	47.61174340465223	19.980661439211953	20.533702928853657	11.87389222728216
13	21.784188977179387	45.64409255473361	21.232817346816255	11.338901121270748
14	13.541843095251158	48.5068674950759	26.671549017128783	11.279740392544158
15	11.126351890240944	21.62594993125746	55.261289242687006	11.986408935814586
16	13.17447405396508	15.29098093100221	52.64787995461163	18.886665060421077
17	14.439750284472455	15.09353995058807	29.476594536697544	40.99011522824193
18	20.907910441471035	20.63954020027179	36.3349451451227	22.117604213134477
19	30.39271114376614	21.87563365195839	25.98237423772912	21.74928096654636
20	32.1808918797924	21.929307700198237	23.723372702253755	22.166427717755614
21	21.909189871746857	28.52437716236837	27.828920531396932	21.737512434487844
22	22.199824903327077	24.252559059344414	24.665252852477476	28.882363184851034
23	17.3014915024042	31.877216042417604	24.529596665370537	26.291695789807655
24	18.637935545022188	23.405383785348334	40.781064749986285	17.175615919643192
25	15.673299188686943	23.672720304137037	39.134901569747235	21.51907893742878
26	14.345840579329847	36.046059489929554	27.707338872495114	21.90076105824549
27	17.064291967738317	34.32864898047139	29.443117834017578	19.16394121777271
28	13.365633182807446	27.80776898053501	41.747674720954585	17.07892311570296
29	13.613885595555312	24.426303941424518	41.109390890997155	20.850419572023018
30	17.853419752526854	29.726198740289966	33.13676704111273	19.283614466070446
31	31.205455509780204	25.36874071140776	23.51289091604504	19.912912862766987
32	32.15846805519442	26.881633217794977	24.823650932616406	16.136247794394205
33	28.570656119517395	25.77761768333266	25.728237558951996	19.923488638197952
34	19.28687466751909	26.841159009566702	29.77239818033049	24.09956814258372
35	19.348898012151807	25.09416813574525	32.3797641681596	23.17716968394335
36	30.778925739767537	22.786899715408268	28.010299055893373	18.423875488930822
37	20.75786165772497	31.748000741099453	28.71354836349815	18.780589237677432
38	20.185736062042427	35.076984488597645	22.206027237790344	22.53125221156958
39	19.86035205404619	31.676832928988834	26.447946908016995	22.01486810894798
40	23.908727082175364	24.61603176231382	24.249378375004273	27.22586278050654
41	16.950184917035823	22.10058755191473	26.4869102911837	34.46231723986574
42	22.505091082871935	24.423043739975874	23.7998681606341	29.271997016518085
43	22.323871592592504	25.91033173742496	24.722584687708483	27.043211982274045
44	19.733204197549124	31.111545804637597	27.64420228834335	21.51104770946993
45	16.563731769708912	38.87329028277079	21.747452073050777	22.81552587446952
46	23.17009266128654	32.508899952369255	25.30242344291586	19.01858394342835
47	22.940844837471005	26.90827144914364	26.090358471076847	24.060525242308508
48	23.90562591494373	22.480917881886878	30.144776799452288	23.468679403717108
49	20.96381096874898	21.448070159535174	33.383429111690525	24.204689760025317
50	19.098260086148837	33.3462151049109	26.15246133281806	21.403063476122206
51	18.102546853468258	34.77450140785041	23.39130925714322	23.731642481538113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1002.0
1	1440.5
2	1879.0
3	5256.5
4	8634.0
5	6268.0
6	3902.0
7	4274.0
8	4646.0
9	5143.0
10	5640.0
11	5537.5
12	5435.0
13	5180.0
14	4925.0
15	4728.0
16	4531.0
17	4451.0
18	4371.0
19	4077.0
20	3783.0
21	3759.5
22	3736.0
23	3896.5
24	4057.0
25	4669.5
26	6886.0
27	8490.0
28	9544.5
29	10599.0
30	12386.0
31	14173.0
32	15021.5
33	15870.0
34	18083.0
35	20296.0
36	28037.0
37	35778.0
38	55504.0
39	75230.0
40	104013.0
41	132796.0
42	142753.5
43	152711.0
44	146041.0
45	139371.0
46	131526.5
47	123682.0
48	113468.5
49	103255.0
50	94621.0
51	85987.0
52	77032.5
53	68078.0
54	60098.0
55	52118.0
56	47779.5
57	43441.0
58	40042.0
59	36643.0
60	31772.5
61	26902.0
62	23230.5
63	19559.0
64	16233.0
65	12907.0
66	10031.5
67	7156.0
68	5704.0
69	4252.0
70	3603.5
71	2955.0
72	2351.5
73	1748.0
74	1351.0
75	695.5
76	437.0
77	326.0
78	215.0
79	157.0
80	99.0
81	68.0
82	37.0
83	25.5
84	14.0
85	7.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	3.0
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1257591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.663229595785417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54534234941877	16.436636722413933
2	6.369503784299457	2.632290382124075
3	2.0138713223735953	1.2483925653172074
4	1.100093736327579	0.9092595780248874
5	0.7558032053870822	0.7808667581071921
6	0.5462362801347889	0.6772203401983742
7	0.4252531206281909	0.6150972007505178
8	0.3503396126875867	0.5791318282769713
9	0.3246141415942064	0.6036818884019894
>10	6.020325116194121	33.22238905975941
>50	2.167062224633487	30.74509907829922
>100	0.37844987656474	10.450242550983075
>500	0.0019407685977678973	0.27149424405634726
>1k	7.763074391071589E-4	0.4172269003193851
>5k	3.8815371955357945E-4	0.41097090296742195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5124	0.4074456639718319	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3402	0.2705172031288392	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1800	0.1431307953062641	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.951710850348006E-5	0.0	0.0	0.01200708338402549	0.0
2	7.951710850348006E-5	0.0	0.0	0.05176563763576552	0.0
3	7.951710850348006E-5	0.0	0.0	0.07744966368238959	0.0
4	7.951710850348006E-5	0.0	0.0	0.13358874228584652	0.0
5	7.951710850348006E-5	0.0	0.0	0.25922577372134503	0.0
6	7.951710850348006E-5	0.0	0.0	0.39750602540889685	0.0
7	7.951710850348006E-5	0.0	0.0	0.4998445440528757	0.0
8	7.951710850348006E-5	0.0	0.0	0.7775182869470281	0.0
9	1.5903421700696013E-4	0.0	0.0	1.1056853937408904	0.0
10	1.5903421700696013E-4	0.0	0.0	1.7025408101680117	0.0
11	1.5903421700696013E-4	0.0	0.0	2.008204575255389	0.0
12	1.5903421700696013E-4	0.0	0.0	2.404040741385713	0.0
13	1.5903421700696013E-4	0.0	0.0	2.58494216323113	0.0
14	1.5903421700696013E-4	0.0	0.0	2.6691507811363153	0.0
15	1.5903421700696013E-4	0.0	0.0	2.74763416722925	0.0
16	1.5903421700696013E-4	0.0	0.0	2.902294943268519	0.0
17	1.5903421700696013E-4	0.0	0.0	3.060374954973437	0.0
18	1.5903421700696013E-4	0.0	0.0	3.387110753814237	0.0
19	1.5903421700696013E-4	0.0	0.0	3.4888926526986914	0.0
20	1.5903421700696013E-4	0.0	0.0	3.605226182439283	0.0
21	1.5903421700696013E-4	0.0	0.0	3.7423931946077857	0.0
22	1.5903421700696013E-4	0.0	0.0	3.8704157392983887	0.0
23	1.5903421700696013E-4	0.0	0.0	4.021816313889015	0.0
24	1.5903421700696013E-4	0.0	0.0	4.137275155436068	0.0
25	1.5903421700696013E-4	0.0	0.0	4.225221077440917	0.0
26	1.5903421700696013E-4	0.0	0.0	4.316109132460395	0.0
27	1.5903421700696013E-4	0.0	0.0	4.406122499286334	0.0
28	1.5903421700696013E-4	0.0	0.0	4.49422745550819	7.951710850348006E-5
29	1.5903421700696013E-4	0.0	0.0	4.594578046439581	7.951710850348006E-5
30	1.5903421700696013E-4	0.0	0.0	4.740730491868978	7.951710850348006E-5
31	1.5903421700696013E-4	0.0	0.0	4.882270945005173	7.951710850348006E-5
32	1.5903421700696013E-4	0.0	0.0	4.998763508962771	7.951710850348006E-5
33	1.5903421700696013E-4	0.0	0.0	5.1091332555656015	7.951710850348006E-5
34	1.5903421700696013E-4	0.0	0.0	5.236758214713687	7.951710850348006E-5
35	1.5903421700696013E-4	0.0	0.0	5.405254967632561	7.951710850348006E-5
36	1.5903421700696013E-4	0.0	0.0	5.53661723088031	7.951710850348006E-5
37	1.5903421700696013E-4	0.0	0.0	5.661777159664788	7.951710850348006E-5
38	1.5903421700696013E-4	0.0	0.0	5.777713103862862	7.951710850348006E-5
39	1.5903421700696013E-4	0.0	0.0	5.91408494494633	7.951710850348006E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	75	0.0	45.000004	1
CCGTAGG	30	2.1656651E-6	45.000004	1
CTACGCG	35	1.2120472E-7	45.000004	1
TACGACA	30	2.1656651E-6	45.000004	23
GTCACGA	70	0.0	45.000004	2
GTTCACG	55	1.8189894E-12	45.000004	1
CGTGTAA	30	2.1656651E-6	45.000004	26
GCGTGAT	30	2.1656651E-6	45.000004	16
GCTACGA	60	0.0	45.000004	2
CGCGTCA	30	2.1656651E-6	45.000004	39
ATTGCGA	125	0.0	45.000004	2
TATCGTA	30	2.1656651E-6	45.000004	26
TAGTCTA	30	2.1656651E-6	45.000004	2
GTACCGA	20	7.0335396E-4	45.0	2
CCTAGGT	20	7.0335396E-4	45.0	39
AAGACGC	20	7.0335396E-4	45.0	38
ATTCGAA	20	7.0335396E-4	45.0	3
TAACGGC	20	7.0335396E-4	45.0	17
TATGCGT	20	7.0335396E-4	45.0	20
GTAGCGC	20	7.0335396E-4	45.0	34
>>END_MODULE