FastQCFastQC Report
Thu 26 May 2016
SRR1546881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546881_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2098787
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133570.6364152246035448No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.34996405066354996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA2700.043.33333231
CGTTTTT129500.043.2972951
GTTTTTA95900.040.5656932
GAGCGAT19550.039.7058837
TATACGA1100.038.8636362
GAGGGGA121950.038.3025867
TAGCATA8050.037.73291830
AGGGGAA124900.037.524028
TATCGCG601.5643309E-1037.5000041
TGCGTAG1800.037.51
TCACGAC3250.037.38461725
TTATTTT112550.036.9835626
TTTTATT113500.036.7136574
GCGCGAC6500.036.6923079
TATTTTT114450.036.3892567
GACCGAT7450.036.241619
CGACGGT3300.036.13636428
TTTATTT115650.036.0116735
TTTAGCG1000.036.0000041
CTATACG250.00210750436.0000041
Produced by FastQC (version 0.11.5)