##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546881_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2098787
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.641925550329784	31.0	31.0	33.0	30.0	34.0
2	31.99510574441332	31.0	31.0	34.0	30.0	34.0
3	31.983904512463628	33.0	31.0	34.0	30.0	34.0
4	35.78074716491002	37.0	35.0	37.0	35.0	37.0
5	35.79024407907996	37.0	35.0	37.0	35.0	37.0
6	35.837759143733976	37.0	35.0	37.0	35.0	37.0
7	36.050779807574564	37.0	35.0	37.0	35.0	37.0
8	13.27031375742274	0.0	0.0	35.0	0.0	37.0
9	24.904546769157612	18.0	17.0	38.0	17.0	39.0
10	32.503132523691065	32.0	32.0	37.0	27.0	39.0
11	35.45967551733454	37.0	34.0	37.0	32.0	39.0
12	36.59179754782167	37.0	35.0	39.0	33.0	39.0
13	36.88163258110518	39.0	37.0	39.0	33.0	39.0
14	38.0513915895229	40.0	37.0	41.0	33.0	41.0
15	38.30272724197358	40.0	37.0	41.0	33.0	41.0
16	38.358919223341864	40.0	37.0	41.0	34.0	41.0
17	38.323676961978514	40.0	37.0	41.0	34.0	41.0
18	38.26288661021819	40.0	37.0	41.0	33.0	41.0
19	38.2347260584328	40.0	37.0	41.0	34.0	41.0
20	38.19789954864405	40.0	37.0	41.0	34.0	41.0
21	38.13510232338965	40.0	37.0	41.0	33.0	41.0
22	38.07178003294284	40.0	37.0	41.0	33.0	41.0
23	37.93010962999104	40.0	36.0	41.0	33.0	41.0
24	37.78834965148917	40.0	35.0	41.0	33.0	41.0
25	37.66218963620415	40.0	35.0	41.0	33.0	41.0
26	37.59606668042064	40.0	35.0	41.0	33.0	41.0
27	37.53991758096463	40.0	35.0	41.0	33.0	41.0
28	37.432470755726996	40.0	35.0	41.0	33.0	41.0
29	37.393182824174154	40.0	35.0	41.0	33.0	41.0
30	37.27628006081608	40.0	35.0	41.0	33.0	41.0
31	37.168114248849456	40.0	35.0	41.0	32.0	41.0
32	36.97522759574935	40.0	35.0	41.0	31.0	41.0
33	36.753723460265384	40.0	35.0	41.0	31.0	41.0
34	36.49929173374907	40.0	35.0	41.0	30.0	41.0
35	36.30722221931049	39.0	35.0	41.0	30.0	41.0
36	36.1720517613269	39.0	35.0	41.0	29.0	41.0
37	36.10441936223161	39.0	35.0	41.0	29.0	41.0
38	36.00339338865735	39.0	35.0	41.0	29.0	41.0
39	35.90920422129545	39.0	35.0	41.0	28.0	41.0
40	35.79175066359759	39.0	35.0	41.0	27.0	41.0
41	35.711618663542325	39.0	35.0	40.0	27.0	41.0
42	35.64875616248814	39.0	35.0	40.0	27.0	41.0
43	35.53333473096603	39.0	35.0	40.0	26.0	41.0
44	35.35397589178893	38.0	35.0	40.0	26.0	41.0
45	35.242999408706076	38.0	35.0	40.0	25.0	41.0
46	35.20831508866788	38.0	34.0	40.0	25.0	41.0
47	35.15939778548276	38.0	34.0	40.0	25.0	41.0
48	35.06103382572886	38.0	34.0	40.0	24.0	41.0
49	34.951861718221046	38.0	34.0	40.0	24.0	41.0
50	34.819894062618076	38.0	34.0	40.0	24.0	41.0
51	34.46089431657429	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	7.0
13	9.0
14	11.0
15	36.0
16	79.0
17	270.0
18	587.0
19	1346.0
20	2561.0
21	4369.0
22	7153.0
23	11128.0
24	17342.0
25	25771.0
26	33192.0
27	35987.0
28	36605.0
29	38072.0
30	45419.0
31	56225.0
32	73001.0
33	99091.0
34	147082.0
35	212583.0
36	232372.0
37	379803.0
38	447915.0
39	190725.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.89062968276438	22.350338552697345	26.656349596219147	16.10268216831913
2	35.54424531884369	23.815041736012276	27.54352871444315	13.097184230700876
3	30.326278941121704	23.853778396759655	32.736385350204664	13.083557311913976
4	26.076300262961418	26.301430302360362	32.03312198903462	15.589147445643603
5	23.212312635822503	30.52201104733353	31.15604394347783	15.10963237336614
6	20.800967415940733	39.3984239467845	29.292729562361497	10.507879074913271
7	83.49541902060571	4.480063960754474	9.950461862018395	2.07405515662142
8	31.608972230150084	63.614554502195794	3.703806055593064	1.072667212061062
9	79.18459567359622	5.327648779985773	11.831643706579085	3.6561118398389167
10	41.591690819506695	24.82877014199154	19.563871893622363	14.015667144879398
11	35.80315677579478	24.05808688542477	24.34449041279558	15.794265925984865
12	31.47379891337234	22.87325964950231	28.93585675916613	16.71708467795922
13	24.26892295406823	28.481689661695064	29.375872825589255	17.873514558647447
14	19.562823669100293	31.66490930237323	31.6757727201474	17.09649430837908
15	19.809775837185956	23.559894358026803	39.65338073849323	16.976949066294004
16	22.14803121993799	21.18923930822899	37.72107412519708	18.941655346635937
17	21.84538021247511	20.891638837099716	30.579091637217115	26.68388931320806
18	24.52545208256007	23.133266977544647	32.48362030067844	19.857660639216842
19	27.289715440394858	24.35397208006339	28.504750601180582	19.851561878361167
20	28.567358193089625	24.523307986946747	26.794810526270652	20.114523293692976
21	25.827680464954284	26.00039927824977	29.518240774313924	18.653679482482026
22	23.940304566399544	23.193063421871777	29.67423564182549	23.192396369903186
23	21.980601175822034	27.05596137197343	28.94414726220431	22.019290190000223
24	22.314603625808623	24.930066748078772	33.18092784070036	19.574401785412242
25	22.199108342104275	24.230758052151074	32.347494052517	21.22263955322765
26	20.342845653227318	28.041197129580087	29.360006518050664	22.255950699141934
27	20.884825377706264	26.319821878065756	31.357779517406957	21.437573226821016
28	18.94761116778406	26.55362359305637	34.39677299316224	20.10199224599733
29	20.63544323459217	26.200419575688244	33.04565923078426	20.118477958935326
30	23.327855566096037	25.233813626632905	32.022353864398816	19.41597694287224
31	27.363138803508885	24.03869473176649	27.892730419999744	20.70543604472488
32	27.421553497329647	25.263211559820032	28.18199274152165	19.133242201328674
33	26.222051118098218	24.75768146076758	27.923033638001378	21.09723378313283
34	22.354483804216436	26.61670765065726	30.001805804972108	21.027002740154195
35	22.282394545039587	26.569108728041485	29.90713207200159	21.24136465491734
36	26.512028138157902	27.045907945875403	26.436937145122396	20.005126770844303
37	22.789163454890847	29.652842332261443	27.087979866465727	20.47001434638198
38	23.096579119272228	30.67819650112184	24.726520604520612	21.498703775085325
39	23.171146000046694	27.71419872526369	26.079587876235177	23.03506739845444
40	24.046079949990162	25.40962946692542	27.065585978948793	23.47870460413563
41	20.98240555139707	25.678308470559426	27.387247967516476	25.952038010527033
42	22.982608525781796	26.715431342008504	25.509449029367914	24.792511102841786
43	22.10414872971864	26.138145509763493	26.982490362290218	24.775215398227644
44	22.051880443322737	27.674032667440763	27.891729842046857	22.38235704718964
45	21.394548374846995	29.160319746596485	26.584832095872517	22.860299782684
46	23.454214267574557	27.808443639111545	27.38386506110434	21.353477032209558
47	22.833379471094496	27.040523883557505	28.02876137502281	22.097335270325193
48	22.467310880046426	26.263122460735655	29.25542229868967	22.01414436052825
49	22.67705107759863	25.182545918189884	29.64907825329583	22.49132475091565
50	21.690767095469905	27.467580083162325	29.061500762106874	21.780152059260896
51	21.44500609161387	28.68066173461147	27.307106438147365	22.567225735627293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1327.0
1	2423.0
2	3519.0
3	20310.0
4	37101.0
5	25750.0
6	14399.0
7	14176.5
8	13954.0
9	14233.0
10	14512.0
11	14205.0
12	13898.0
13	13344.0
14	12790.0
15	12183.0
16	11576.0
17	10899.5
18	10223.0
19	10124.0
20	10025.0
21	10180.5
22	10336.0
23	11107.5
24	11879.0
25	13757.0
26	17772.5
27	19910.0
28	22271.5
29	24633.0
30	28826.0
31	33019.0
32	37019.0
33	41019.0
34	45071.0
35	49123.0
36	56180.0
37	63237.0
38	76834.0
39	90431.0
40	106277.0
41	122123.0
42	131405.5
43	140688.0
44	144306.5
45	147925.0
46	154139.0
47	160353.0
48	161575.5
49	162798.0
50	160500.5
51	158203.0
52	148432.5
53	138662.0
54	130351.5
55	122041.0
56	114633.5
57	107226.0
58	100545.5
59	93865.0
60	85000.5
61	76136.0
62	66462.0
63	56788.0
64	47432.5
65	38077.0
66	31352.5
67	24628.0
68	20701.5
69	16775.0
70	14695.5
71	12616.0
72	10637.5
73	8659.0
74	6617.0
75	3407.5
76	2240.0
77	1654.0
78	1068.0
79	771.5
80	475.0
81	329.0
82	183.0
83	134.0
84	85.0
85	57.5
86	30.0
87	22.5
88	15.0
89	9.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2098787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.567665668922935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84710591987553	19.1366578044584
2	5.897525752058259	3.015719334049777
3	2.590124018542474	1.9867027484142332
4	1.7379369859724443	1.7773996724399623
5	1.2648091264598502	1.616910844016396
6	1.0647359241238785	1.6333687280214615
7	0.9100736220357319	1.6287920671620728
8	0.790694391709238	1.6172967882811349
9	0.7661504987585406	1.7629811823903225
>10	9.486444416708864	52.47587783476734
>50	0.5922248927917317	9.537658187709642
>100	0.04825161201203044	2.0699157366720016
>500	0.003334413108787579	0.59932971175991
>1k	1.9614194757573995E-4	0.10320647199413395
>5k	1.9614194757573995E-4	0.3683437982492293
>10k+	1.9614194757573995E-4	0.6698390896140171
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13357	0.6364152246035448	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7345	0.34996405066354996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005479355456270693	0.0
2	0.0	0.0	0.0	0.0208691973030136	0.0
3	0.0	0.0	0.0	0.0374025568101956	0.0
4	0.0	0.0	0.0	0.0563658913458107	0.0
5	0.0	0.0	0.0	0.11087356649340786	0.0
6	0.0	0.0	0.0	0.20216439305179612	0.0
7	0.0	0.0	0.0	0.26177025110218427	0.0
8	0.0	0.0	0.0	0.3986588443705817	0.0
9	0.0	0.0	0.0	0.6015855825293371	0.0
10	0.0	0.0	0.0	0.8967560786301801	0.0
11	0.0	0.0	0.0	1.073858376290686	0.0
12	0.0	0.0	0.0	1.2444330939728518	0.0
13	0.0	0.0	0.0	1.3305780910592642	0.0
14	0.0	0.0	0.0	1.3725070719420314	0.0
15	4.7646569184962554E-5	0.0	0.0	1.4142454665480584	0.0
16	4.7646569184962554E-5	0.0	0.0	1.4949111081782	0.0
17	4.7646569184962554E-5	0.0	0.0	1.5781020179751446	0.0
18	4.7646569184962554E-5	0.0	0.0	1.730380453090285	0.0
19	4.7646569184962554E-5	0.0	0.0	1.788938086618604	0.0
20	4.7646569184962554E-5	0.0	0.0	1.8580256119367997	0.0
21	4.7646569184962554E-5	0.0	0.0	1.9384053741518315	0.0
22	4.7646569184962554E-5	0.0	0.0	2.020595705995892	0.0
23	4.7646569184962554E-5	0.0	0.0	2.123226416020301	0.0
24	4.7646569184962554E-5	0.0	0.0	2.197173891395363	0.0
25	4.7646569184962554E-5	0.0	0.0	2.2656896578833394	0.0
26	4.7646569184962554E-5	0.0	0.0	2.3393989004124762	0.0
27	4.7646569184962554E-5	0.0	0.0	2.4144898934479775	0.0
28	4.7646569184962554E-5	0.0	0.0	2.492106154650281	0.0
29	4.7646569184962554E-5	0.0	0.0	2.5734864948181975	0.0
30	4.7646569184962554E-5	0.0	0.0	2.6826447848209467	0.0
31	4.7646569184962554E-5	0.0	0.0	2.776746758961248	0.0
32	4.7646569184962554E-5	0.0	0.0	2.872992828714872	0.0
33	9.529313836992511E-5	0.0	0.0	2.982389351563546	0.0
34	9.529313836992511E-5	0.0	0.0	3.094358789148208	0.0
35	9.529313836992511E-5	0.0	0.0	3.2169057650919317	0.0
36	9.529313836992511E-5	0.0	0.0	3.323348200651138	0.0
37	9.529313836992511E-5	0.0	0.0	3.436365862757869	0.0
38	1.4293970755488767E-4	0.0	0.0	3.57549384477796	0.0
39	1.4293970755488767E-4	0.0	0.0	3.7627448616748627	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	270	0.0	43.333332	31
CGTTTTT	12950	0.0	43.297295	1
GTTTTTA	9590	0.0	40.565693	2
GAGCGAT	1955	0.0	39.705883	7
TATACGA	110	0.0	38.863636	2
GAGGGGA	12195	0.0	38.302586	7
TAGCATA	805	0.0	37.732918	30
AGGGGAA	12490	0.0	37.52402	8
TATCGCG	60	1.5643309E-10	37.500004	1
TGCGTAG	180	0.0	37.5	1
TCACGAC	325	0.0	37.384617	25
TTATTTT	11255	0.0	36.983562	6
TTTTATT	11350	0.0	36.713657	4
GCGCGAC	650	0.0	36.692307	9
TATTTTT	11445	0.0	36.389256	7
GACCGAT	745	0.0	36.24161	9
CGACGGT	330	0.0	36.136364	28
TTTATTT	11565	0.0	36.011673	5
TTTAGCG	100	0.0	36.000004	1
CTATACG	25	0.002107504	36.000004	1
>>END_MODULE