FastQCFastQC Report
Thu 26 May 2016
SRR1546880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546880_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences487226
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.7538596051934832No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.4306009941998169No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5880.1206832147709687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC302.1629166E-645.00000412
ACACGAC253.8874867E-545.026
CGTATGA351.21001E-745.02
CGCGGTA207.0292805E-445.030
TACGAGT253.8874867E-545.04
TGCGACG207.0292805E-445.01
AGCGATA850.045.08
GCGATAC207.0292805E-445.09
ACGAGTC351.21001E-745.05
CGATAAC207.0292805E-445.016
GAGCGAT3800.043.8157927
CGTTTTT35600.042.9775281
GTTTTTA25250.041.4356422
TAGTAAG501.0786607E-940.51
AGCGATC451.9243089E-840.0000048
CGGTCTA451.9243089E-840.00000431
CACGAGT403.452751E-739.3754
ATTGCGA403.452751E-739.3752
ACGAGGT356.2408817E-638.5714265
GAGGGGA30150.037.9850777
Produced by FastQC (version 0.11.5)