##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546880_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	487226
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702023291039477	31.0	31.0	34.0	30.0	34.0
2	32.05738404764934	33.0	31.0	34.0	30.0	34.0
3	32.032198610090596	33.0	31.0	34.0	30.0	34.0
4	35.81941439906738	37.0	35.0	37.0	35.0	37.0
5	35.82752357222316	37.0	35.0	37.0	35.0	37.0
6	35.8733647219155	37.0	35.0	37.0	35.0	37.0
7	36.069924429320274	37.0	35.0	37.0	35.0	37.0
8	13.241949321259538	0.0	0.0	35.0	0.0	37.0
9	24.861546797584694	18.0	17.0	38.0	17.0	39.0
10	32.61061191315734	32.0	32.0	37.0	27.0	39.0
11	35.55961094030286	37.0	35.0	37.0	32.0	39.0
12	36.67251747648935	37.0	35.0	39.0	33.0	39.0
13	36.949943147533176	39.0	37.0	39.0	33.0	39.0
14	38.15864711653319	40.0	37.0	41.0	33.0	41.0
15	38.42144713131073	40.0	38.0	41.0	34.0	41.0
16	38.48418598350663	40.0	38.0	41.0	34.0	41.0
17	38.462177716295926	40.0	38.0	41.0	34.0	41.0
18	38.37789034246941	40.0	37.0	41.0	34.0	41.0
19	38.34166690611749	40.0	37.0	41.0	34.0	41.0
20	38.28464203470258	40.0	37.0	41.0	34.0	41.0
21	38.21859670871423	40.0	37.0	41.0	34.0	41.0
22	38.16235381527258	40.0	37.0	41.0	34.0	41.0
23	37.9991585013936	40.0	36.0	41.0	33.0	41.0
24	37.82177264760091	40.0	35.0	41.0	33.0	41.0
25	37.69989286286036	40.0	35.0	41.0	33.0	41.0
26	37.62668658897514	40.0	35.0	41.0	33.0	41.0
27	37.55899520961525	40.0	35.0	41.0	33.0	41.0
28	37.45306079724809	40.0	35.0	41.0	33.0	41.0
29	37.42169958089264	40.0	35.0	41.0	33.0	41.0
30	37.30160336271053	40.0	35.0	41.0	33.0	41.0
31	37.17379614388395	40.0	35.0	41.0	32.0	41.0
32	36.950583507448286	40.0	35.0	41.0	31.0	41.0
33	36.706452447119	40.0	35.0	41.0	31.0	41.0
34	36.423803327408635	40.0	35.0	41.0	30.0	41.0
35	36.220636829725834	40.0	35.0	41.0	29.0	41.0
36	36.08062788110651	40.0	35.0	41.0	28.0	41.0
37	36.009761383834196	39.0	35.0	41.0	28.0	41.0
38	35.907342793693275	39.0	35.0	41.0	27.0	41.0
39	35.810227697208276	39.0	35.0	41.0	27.0	41.0
40	35.69356109895613	39.0	35.0	41.0	26.0	41.0
41	35.6199894915296	39.0	35.0	41.0	26.0	41.0
42	35.5535028918818	39.0	35.0	41.0	26.0	41.0
43	35.44339793032392	39.0	35.0	40.0	25.0	41.0
44	35.199488533042164	38.0	35.0	40.0	24.0	41.0
45	35.12249756786378	38.0	35.0	40.0	23.0	41.0
46	35.08920295714925	38.0	34.0	40.0	24.0	41.0
47	35.06314933932097	38.0	34.0	40.0	24.0	41.0
48	34.98271438716325	38.0	34.0	40.0	23.0	41.0
49	34.87119119258824	38.0	34.0	40.0	24.0	41.0
50	34.75513416771683	38.0	34.0	40.0	24.0	41.0
51	34.412769433486716	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	6.0
14	4.0
15	14.0
16	22.0
17	61.0
18	159.0
19	299.0
20	631.0
21	1100.0
22	1778.0
23	2898.0
24	4366.0
25	6625.0
26	8277.0
27	8789.0
28	8520.0
29	8557.0
30	9798.0
31	12453.0
32	16062.0
33	22054.0
34	32656.0
35	48737.0
36	52799.0
37	87394.0
38	107087.0
39	46065.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.67815756958783	21.781678317659566	26.113138461412156	17.427025651340447
2	37.46043930332125	23.265999761917467	26.284722079691967	12.988838855069309
3	29.58093369401469	24.08471633287222	32.78704338438425	13.547306588728844
4	26.49427575704088	25.759093316038147	31.901212168480335	15.84541875844064
5	23.15845213514878	29.649485043901596	31.50221868291101	15.68984413803861
6	21.060862926034325	38.822230340745364	29.640659570712565	10.476247162507748
7	80.70402646820982	5.219138551719325	11.769076362919877	2.307758617150973
8	30.269525846321834	64.00746265593381	4.371687882009581	1.3513236157347923
9	75.14151543636834	6.438490556743688	13.919413167605999	4.500580839281976
10	40.92926075373646	25.235927475134744	20.233526125453075	13.601285645675723
11	35.08453982340844	23.512702524085334	26.010927167269397	15.391830485236829
12	30.688222713894582	22.302996966500146	30.6728294467044	16.33595087290087
13	23.301506898236138	28.980391030035342	30.84564452635943	16.87245754536909
14	18.933102913227128	31.643221010373008	32.46583720901594	16.957838867383927
15	18.19361035741114	23.64857376248394	41.252314121167586	16.90550175893733
16	20.416192896109813	21.576024267998832	39.50076555848826	18.507017277403097
17	21.304897521889227	20.586339809451875	31.62680152537016	26.48196114328874
18	23.370468735248117	22.528970128851906	33.82803873356512	20.27252240233485
19	26.860635516167036	23.46960137595284	30.059561681847846	19.610201426032273
20	28.156132882892127	23.87557314264838	28.45496750994405	19.51332646451544
21	24.83200814406456	25.513827258808025	30.70669463452279	18.947469962604625
22	23.775414284130978	23.323262715864917	30.00373543283815	22.897587567165957
23	21.57232988387319	26.60346533230985	30.015844803027754	21.808359980789202
24	21.90646640368125	24.28954940828281	34.43391773017039	19.370066457865548
25	21.40587735465677	24.25260556702639	33.42658232524537	20.91493475307147
26	19.630931025848376	27.922360465164008	30.5076083788632	21.93910013012442
27	20.327732920656942	27.23664172273237	31.9217365247339	20.513888831876788
28	19.374787059803868	25.819024436298555	35.57876632199431	19.227422181903265
29	20.444926994864808	25.35106911371725	34.19850336394199	20.005500527475956
30	21.563709654246694	26.18846284886275	32.67846953980288	19.56935795708768
31	26.18969431023796	24.50156600838215	28.66801032785607	20.640729353523827
32	26.638767225065983	25.402380004351166	29.406066178734303	18.552786591848548
33	25.86171509730597	24.98286216252827	28.619367603535117	20.53605513663064
34	21.61974114681893	26.20139319330249	30.765599536970523	21.413266122908055
35	22.177182662665786	25.8434484202403	30.746101398529635	21.23326751856428
36	26.266044915501226	26.773407002089378	27.23356306929433	19.726985013115065
37	22.78819274833445	29.135349919749764	27.911072069224545	20.165385262691235
38	22.562630073107755	30.823683465168116	25.705524746216334	20.90816171550779
39	22.390020237015264	28.858065866764086	26.92672394330352	21.825189952917125
40	23.758994799128125	25.803836412670915	27.80229298108065	22.63487580712031
41	20.64052410996129	25.632252794391103	28.012462389117164	25.714760706530438
42	22.534922192165443	27.391600612446794	26.550512493175653	23.522964702212114
43	22.65560540693641	26.409099678588582	27.739488450944737	23.19580646353027
44	21.678030318579058	28.695718208798382	28.588375825592234	21.03787564703033
45	21.187498204118825	29.604126216581218	27.27461178180146	21.933763797498493
46	22.645958959497236	28.78889878618957	28.021082618743666	20.544059635569532
47	22.47971167384335	27.188204241973953	28.726915230303803	21.605168853878897
48	22.82164744902776	26.034735420523536	29.546863262633767	21.596753867814936
49	22.179440341853677	25.461900637486508	30.435362644850645	21.923296375809173
50	20.89748905025594	28.989216503224373	28.95535131540599	21.157943131113694
51	20.641755571336507	29.367685632540137	27.4509160020196	22.539642794103763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	701.0
2	1001.0
3	5538.5
4	10076.0
5	7040.5
6	4005.0
7	3936.5
8	3868.0
9	3892.0
10	3916.0
11	3817.0
12	3718.0
13	3601.5
14	3485.0
15	3281.5
16	3078.0
17	2970.5
18	2863.0
19	2861.0
20	2859.0
21	2905.0
22	2951.0
23	3066.0
24	3181.0
25	3539.0
26	4381.0
27	4865.0
28	5612.5
29	6360.0
30	7548.5
31	8737.0
32	9720.0
33	10703.0
34	11577.5
35	12452.0
36	14217.5
37	15983.0
38	19070.0
39	22157.0
40	25652.0
41	29147.0
42	31250.0
43	33353.0
44	34076.5
45	34800.0
46	34971.5
47	35143.0
48	35111.5
49	35080.0
50	34645.5
51	34211.0
52	32049.5
53	29888.0
54	28181.0
55	26474.0
56	25131.0
57	23788.0
58	21836.5
59	19885.0
60	17882.0
61	15879.0
62	14303.5
63	12728.0
64	10671.5
65	8615.0
66	7403.0
67	6191.0
68	5320.5
69	4450.0
70	3709.0
71	2968.0
72	2413.5
73	1859.0
74	1471.5
75	825.0
76	566.0
77	441.0
78	316.0
79	217.0
80	118.0
81	89.5
82	61.0
83	45.0
84	29.0
85	25.5
86	22.0
87	15.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	2.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05072465368494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.20971911252245	27.1795694868598
2	9.395422202508941	7.713777796806251
3	5.917836651993828	7.2879444883945
4	4.278818714789246	7.025944356153896
5	3.28884364049749	6.750470735754262
6	2.5067188062500048	6.174157409974965
7	1.933451575520953	5.555871178056077
8	1.5185261696076762	4.986927973438367
9	1.1674700384716379	4.313294198165355
>10	3.757658510313554	20.84758548099973
>50	0.017523729673649093	0.4885494293482212
>100	0.006508813878554882	0.36892983830722925
>500	5.006779906580679E-4	0.12085278268782775
>1k	0.0010013559813161358	1.1861248450534931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3673	0.7538596051934832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2098	0.4306009941998169	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	588	0.1206832147709687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0524356253566106E-4	0.0	0.0	0.003078653438034916	0.0
2	2.0524356253566106E-4	0.0	0.0	0.018061433503138175	0.0
3	2.0524356253566106E-4	0.0	0.0	0.036943841256418995	0.0
4	2.0524356253566106E-4	0.0	0.0	0.051310890633915265	0.0
5	2.0524356253566106E-4	0.0	0.0	0.09831166645458166	0.0
6	2.0524356253566106E-4	0.0	0.0	0.18102482215645307	0.0
7	2.0524356253566106E-4	0.0	0.0	0.23069376429008304	0.0
8	2.0524356253566106E-4	0.0	0.0	0.3304421356824143	0.0
9	2.0524356253566106E-4	0.0	0.0	0.4851957818343028	0.0
10	2.0524356253566106E-4	0.0	0.0	0.723073070813134	0.0
11	2.0524356253566106E-4	0.0	0.0	0.8815211010906643	0.0
12	2.0524356253566106E-4	0.0	0.0	1.0451002204315862	0.0
13	2.0524356253566106E-4	0.0	0.0	1.1183721722568172	0.0
14	2.0524356253566106E-4	0.0	0.0	1.153058334325344	0.0
15	2.0524356253566106E-4	0.0	0.0	1.1895916884566915	0.0
16	2.0524356253566106E-4	0.0	0.0	1.2523962185926039	0.0
17	2.0524356253566106E-4	0.0	0.0	1.325462926855299	0.0
18	2.0524356253566106E-4	0.0	0.0	1.4566135633155866	0.0
19	2.0524356253566106E-4	0.0	0.0	1.5075139668244306	0.0
20	2.0524356253566106E-4	0.0	0.0	1.5680608177724507	0.0
21	2.0524356253566106E-4	0.0	0.0	1.6345597320340048	0.0
22	2.0524356253566106E-4	0.0	0.0	1.710089363047128	0.0
23	2.0524356253566106E-4	0.0	0.0	1.7868504554354652	0.0
24	2.0524356253566106E-4	0.0	0.0	1.853554613259555	0.0
25	2.0524356253566106E-4	0.0	0.0	1.9110228107695402	0.0
26	2.0524356253566106E-4	0.0	0.0	1.969927713217275	0.0
27	2.0524356253566106E-4	0.0	0.0	2.025753962226975	0.0
28	2.0524356253566106E-4	0.0	0.0	2.0943053121138857	0.0
29	2.0524356253566106E-4	0.0	0.0	2.165935315438831	0.0
30	2.0524356253566106E-4	0.0	0.0	2.262194546268056	0.0
31	2.0524356253566106E-4	0.0	0.0	2.341829048531893	0.0
32	2.0524356253566106E-4	0.0	0.0	2.438704010048725	0.0
33	2.0524356253566106E-4	0.0	0.0	2.5242905756260954	0.0
34	2.0524356253566106E-4	0.0	0.0	2.6002306937642903	0.0
35	2.0524356253566106E-4	0.0	0.0	2.6981318730938004	0.0
36	2.0524356253566106E-4	0.0	0.0	2.7956225652982396	0.0
37	2.0524356253566106E-4	0.0	0.0	2.884287784313645	0.0
38	2.0524356253566106E-4	0.0	0.0	2.993682603145152	0.0
39	2.0524356253566106E-4	0.0	0.0	3.1238070217927616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	30	2.1629166E-6	45.000004	12
ACACGAC	25	3.8874867E-5	45.0	26
CGTATGA	35	1.21001E-7	45.0	2
CGCGGTA	20	7.0292805E-4	45.0	30
TACGAGT	25	3.8874867E-5	45.0	4
TGCGACG	20	7.0292805E-4	45.0	1
AGCGATA	85	0.0	45.0	8
GCGATAC	20	7.0292805E-4	45.0	9
ACGAGTC	35	1.21001E-7	45.0	5
CGATAAC	20	7.0292805E-4	45.0	16
GAGCGAT	380	0.0	43.815792	7
CGTTTTT	3560	0.0	42.977528	1
GTTTTTA	2525	0.0	41.435642	2
TAGTAAG	50	1.0786607E-9	40.5	1
AGCGATC	45	1.9243089E-8	40.000004	8
CGGTCTA	45	1.9243089E-8	40.000004	31
CACGAGT	40	3.452751E-7	39.375	4
ATTGCGA	40	3.452751E-7	39.375	2
ACGAGGT	35	6.2408817E-6	38.571426	5
GAGGGGA	3015	0.0	37.985077	7
>>END_MODULE