FastQCFastQC Report
Thu 26 May 2016
SRR1546879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546879_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1266030
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190151.5019391325639992No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104790.8277055046088956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG302.165676E-645.0000042
GCGATAT950.045.09
CGTTTTT168850.043.854011
GTTTTTA112100.042.952722
ATTGCGA1250.041.42
GAGCGAT10000.040.7250027
CGTAAGA1050.040.7142832
ACGTTAG501.0822987E-940.51
TATTTTT125450.039.4758877
TAACGAG1600.039.3750043
TTATTTT125800.039.348176
ACGACGG2300.039.13043627
AGCGATA2500.038.78
TTTTATT128200.038.6817474
TTTATTT128750.038.568935
CGGTCTA2400.038.43750431
AGACTTA9050.038.0386778
ATAACGC1250.037.80000311
TTTTTAT132800.037.5282363
CTCACGA2400.037.50000424
Produced by FastQC (version 0.11.5)