##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546879_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1266030
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647902498361017	31.0	31.0	34.0	30.0	34.0
2	31.988304384572245	31.0	31.0	34.0	30.0	34.0
3	31.873996666745654	31.0	31.0	34.0	30.0	34.0
4	35.72928208652244	37.0	35.0	37.0	35.0	37.0
5	35.76567616881117	37.0	35.0	37.0	35.0	37.0
6	35.825933824632905	37.0	35.0	37.0	35.0	37.0
7	36.043647464909995	37.0	35.0	37.0	35.0	37.0
8	13.202867230634345	0.0	0.0	35.0	0.0	37.0
9	24.819365259906952	18.0	17.0	38.0	17.0	39.0
10	32.55834537886148	32.0	32.0	37.0	27.0	39.0
11	35.524448077849655	37.0	34.0	37.0	32.0	39.0
12	36.7184845540785	38.0	35.0	39.0	33.0	39.0
13	37.03581589693767	39.0	37.0	39.0	33.0	39.0
14	38.27844521851773	40.0	38.0	41.0	34.0	41.0
15	38.48566937592316	40.0	38.0	41.0	34.0	41.0
16	38.52795352400812	40.0	38.0	41.0	34.0	41.0
17	38.48453670134199	40.0	38.0	41.0	34.0	41.0
18	38.37711428639132	40.0	38.0	41.0	34.0	41.0
19	38.29863510343357	40.0	37.0	41.0	34.0	41.0
20	38.20324557869877	40.0	37.0	41.0	34.0	41.0
21	38.138183929290776	40.0	37.0	41.0	33.0	41.0
22	38.08326421964724	40.0	36.0	41.0	33.0	41.0
23	37.87409461071223	39.0	36.0	41.0	33.0	41.0
24	37.5445076341003	39.0	35.0	41.0	33.0	41.0
25	37.421565049801345	39.0	35.0	41.0	33.0	41.0
26	37.34620664597205	39.0	35.0	41.0	33.0	41.0
27	37.28169711618208	39.0	35.0	41.0	33.0	41.0
28	37.11488827278974	39.0	35.0	41.0	33.0	41.0
29	37.00029146228762	39.0	35.0	41.0	32.0	41.0
30	36.80967117682835	39.0	35.0	41.0	31.0	41.0
31	36.61172484064359	39.0	35.0	41.0	31.0	41.0
32	36.24016413513108	39.0	35.0	41.0	30.0	41.0
33	35.80535137398008	39.0	35.0	41.0	26.0	41.0
34	35.354565847570754	39.0	35.0	41.0	23.0	41.0
35	34.99826781355892	39.0	35.0	41.0	21.0	41.0
36	34.805776324415696	39.0	35.0	41.0	20.0	41.0
37	34.72421032676951	39.0	35.0	41.0	18.0	41.0
38	34.597840493511214	39.0	34.0	40.0	18.0	41.0
39	34.47684494048324	38.0	34.0	40.0	18.0	41.0
40	34.346141876574805	38.0	34.0	40.0	18.0	41.0
41	34.23732060061768	38.0	34.0	40.0	17.0	41.0
42	34.15410298334163	38.0	34.0	40.0	17.0	41.0
43	34.02692432248841	38.0	33.0	40.0	15.0	41.0
44	33.82163376855209	38.0	33.0	40.0	15.0	41.0
45	33.72802461237095	38.0	33.0	40.0	15.0	41.0
46	33.68372708387637	38.0	33.0	40.0	15.0	41.0
47	33.639800794609926	37.0	33.0	40.0	15.0	41.0
48	33.5315821899955	37.0	33.0	40.0	15.0	41.0
49	33.425749784760235	37.0	33.0	40.0	15.0	41.0
50	33.33111379667149	37.0	33.0	40.0	15.0	41.0
51	32.983251581716075	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	5.0
13	4.0
14	10.0
15	35.0
16	72.0
17	243.0
18	531.0
19	1207.0
20	2278.0
21	3887.0
22	6411.0
23	10144.0
24	16475.0
25	25757.0
26	33011.0
27	34588.0
28	30980.0
29	28828.0
30	30301.0
31	34782.0
32	42959.0
33	57340.0
34	82971.0
35	120799.0
36	135767.0
37	212881.0
38	245551.0
39	108190.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.26880089729311	20.308918430052998	22.658783757098963	23.763496915554924
2	41.94292394335047	21.571842689351755	24.33733797777304	12.147895389524734
3	28.204544915997253	22.392281383537515	37.083639408228876	12.319534292236362
4	24.81039153890508	24.44973657812216	36.18926881669471	14.55060306627805
5	21.211345702708467	28.232664312851984	35.97110652986106	14.584883454578485
6	19.69471497515857	36.14472010931811	34.46498108259678	9.69558383292655
7	75.44386783883478	4.368932805699707	17.732044264353924	2.455155091111585
8	28.094357953602994	63.96688862033285	6.591313002061562	1.3474404240025908
9	69.92227672330039	5.7302749539900315	19.908453986082478	4.438994336627093
10	36.13445179024194	24.885745203510183	25.074366326232393	13.905436680015482
11	29.524655813843275	22.832634297765455	31.67910713016279	15.963602758228479
12	25.913209007685438	20.745874900278825	36.50174166488946	16.839174427146276
13	22.240231274140424	23.02354604551235	37.37486473464294	17.361357945704288
14	18.586605372700486	26.471647591289305	38.29711776182239	16.644629274187817
15	18.08235191899086	21.99276478440479	42.61960617046989	17.305277126134452
16	20.738213154506607	20.81293492255318	40.47463330252838	17.97421862041184
17	20.78370970672101	20.470131039548825	36.16470383798172	22.58145541574844
18	22.105716294242633	22.072857673198897	36.7559220555595	19.065503976998965
19	24.389074508502958	22.98081404074153	33.84880295095693	18.781308499798584
20	25.553975814159223	22.903722660600458	32.71920886550871	18.823092659731604
21	23.916020947370914	23.50528818432423	34.63622504995932	17.94246581834554
22	22.112588169316684	21.22959171583612	35.233367297773356	21.424452817073846
23	21.073750227087825	23.764207799183275	34.55076104041768	20.611280933311214
24	21.2679004446972	22.995663609867066	36.39692582324274	19.339510122192998
25	21.53803622347022	22.66597158045228	35.393948010710645	20.402044185366854
26	19.757114760313737	24.457951233383096	34.30992946454665	21.475004541756515
27	20.09612726396689	23.080416735780354	36.137295324755335	20.68616067549742
28	19.31763070385378	23.58443322828053	37.67943887585602	19.418497192009667
29	20.5917711270665	23.745882798985807	36.42907356065812	19.233272513289574
30	21.786608532183283	22.900563177807793	36.257908580365395	19.05491970964353
31	24.8114183708127	22.27474862365031	33.0816015418276	19.83223146370939
32	24.79072375852073	23.49644163250476	32.8409279400962	18.871906668878307
33	23.52866835698996	23.86941857617908	32.04276359959874	20.559149467232213
34	21.37998309676706	25.786671721839134	32.92591802721894	19.90742715417486
35	21.113638697345245	26.086190690583948	32.2978918351066	20.50227877696421
36	23.846433338862425	26.548344036081296	29.449855058726886	20.155367566329392
37	21.686215966446294	27.33023704019652	29.774096980324323	21.209450013032864
38	21.617418228635973	28.84789459965404	28.034011832263054	21.500675339446932
39	21.511417580942	26.40980071562285	28.837547293508052	23.241234409927095
40	22.482484617268153	25.163068805636517	29.90821702487303	22.446229552222302
41	20.790897530074325	25.1539852926076	29.888391270349047	24.16672590696903
42	22.09647480707408	26.77464199110606	28.467887806766036	22.660995395053828
43	21.843479222451283	25.671429586976615	29.270396436103407	23.21469475446869
44	21.8032748039146	26.860342961857143	30.117295798677755	21.219086435550498
45	21.329352385014573	27.59097335766135	29.423394390338302	21.656279866985777
46	22.534695070416973	26.368411491038916	30.14375646706634	20.95313697147777
47	21.617418228635973	26.44384414271384	30.46144246186899	21.4772951667812
48	21.72871101000766	25.807603295340552	30.9352858936992	21.528399800952585
49	21.8397668301699	25.198218051704934	31.199102706886883	21.76291241123828
50	20.43277015552554	26.112414397763082	32.00374398710931	21.451071459602066
51	20.718624361192074	26.77116656003412	30.98062447177397	21.529584606999837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	935.0
1	1938.0
2	2941.0
3	26422.0
4	49903.0
5	34113.0
6	18323.0
7	17868.0
8	17413.0
9	17371.0
10	17329.0
11	16836.5
12	16344.0
13	15472.5
14	14601.0
15	13780.0
16	12959.0
17	12152.0
18	11345.0
19	10716.0
20	10087.0
21	9737.5
22	9388.0
23	9463.5
24	9539.0
25	10073.0
26	11569.5
27	12532.0
28	14199.5
29	15867.0
30	17278.0
31	18689.0
32	21209.5
33	23730.0
34	26410.0
35	29090.0
36	31665.5
37	34241.0
38	39957.0
39	45673.0
40	52169.5
41	58666.0
42	63662.5
43	68659.0
44	72642.0
45	76625.0
46	82047.0
47	87469.0
48	87231.5
49	86994.0
50	84337.0
51	81680.0
52	76719.0
53	71758.0
54	69099.0
55	66440.0
56	63822.0
57	61204.0
58	58417.0
59	55630.0
60	51629.0
61	47628.0
62	42474.0
63	37320.0
64	32253.5
65	27187.0
66	23356.0
67	19525.0
68	16844.0
69	14163.0
70	12130.0
71	10097.0
72	8341.5
73	6586.0
74	5078.0
75	2625.5
76	1681.0
77	1272.0
78	863.0
79	661.5
80	460.0
81	295.5
82	131.0
83	111.0
84	91.0
85	62.5
86	34.0
87	24.0
88	14.0
89	13.0
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1266030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.88828639295647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.51392383858057	27.03277378532885
2	8.469822117851237	6.587537364327921
3	4.657915800119995	5.434150908880301
4	3.4701477027644057	5.397923907638488
5	2.6631819369849534	5.178329094100972
6	2.146538775647921	5.008512879658951
7	1.7186987226714325	4.678607370531859
8	1.382634539799948	4.3014630368427165
9	1.1380882584259688	3.9832471920717123
>10	4.780513052027555	26.80368076654084
>50	0.03134232290402144	0.8331643074569828
>100	0.02472084934257643	1.8748750128318508
>500	0.0020600690662232746	0.5228946519452344
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.120138132446549E-4	2.362839721843534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19015	1.5019391325639992	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10479	0.8277055046088956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008451616470383798	0.0
2	0.0	0.0	0.0	0.048656035007069345	0.0
3	0.0	0.0	0.0	0.09123006563825502	0.0
4	0.0	0.0	0.0	0.13088157468622386	0.0
5	0.0	0.0018167026057834333	0.0	0.22661390330402914	0.0
6	0.0	0.0018167026057834333	0.0	0.41831552174908965	0.0
7	0.0	0.0018167026057834333	0.0	0.5566218809980806	0.0
8	0.0	0.0018167026057834333	0.0	0.7529047494925081	0.0
9	0.0	0.0018167026057834333	0.0	1.111585033530011	0.0
10	0.0	0.0018167026057834333	0.0	1.5900887024794041	0.0
11	0.0	0.0018167026057834333	0.0	1.871282671026753	0.0
12	0.0	0.0018167026057834333	0.0	2.198921036626304	0.0
13	0.0	0.0018167026057834333	0.0	2.346626857183479	0.0
14	0.0	0.0018167026057834333	0.0	2.4403845090558676	0.0
15	0.0	0.0018167026057834333	0.0	2.511867807239955	0.0
16	0.0	0.0018167026057834333	0.0	2.5986745969684764	0.0
17	0.0	0.0018167026057834333	0.0	2.685876322046081	0.0
18	0.0	0.0018167026057834333	0.0	2.8542767548952237	0.0
19	0.0	0.0018167026057834333	0.0	2.927813716894544	0.0
20	0.0	0.0018167026057834333	0.0	3.0096443212246156	0.0
21	0.0	0.0018167026057834333	0.0	3.0943974471379034	0.0
22	0.0	0.0018167026057834333	0.0	3.1800984178889915	0.0
23	0.0	0.0018167026057834333	0.0	3.2827026215808472	0.0
24	0.0	0.0018167026057834333	0.0	3.3685615664715685	0.0
25	0.0	0.0018167026057834333	0.0	3.4423354896803393	0.0
26	0.0	0.0018167026057834333	0.0	3.51705725772691	0.0
27	0.0	0.0018167026057834333	0.0	3.6012574741514816	0.0
28	0.0	0.0018167026057834333	0.0	3.688459199229086	0.0
29	0.0	0.0018167026057834333	0.0	3.782611786450558	0.0
30	0.0	0.0018167026057834333	0.0	3.9039359256889647	0.0
31	0.0	0.0018167026057834333	0.0	3.998404461189703	0.0
32	0.0	0.0018167026057834333	0.0	4.112698751214427	0.0
33	0.0	0.0018167026057834333	0.0	4.223043687748316	0.0
34	0.0	0.0018167026057834333	0.0	4.328886361302655	0.0
35	0.0	0.0018167026057834333	0.0	4.447524940167295	0.0
36	0.0	0.0018167026057834333	0.0	4.552498755953651	0.0
37	0.0	0.0018167026057834333	0.0	4.66639811062929	0.0
38	0.0	0.0018167026057834333	0.0	4.798859426711847	0.0
39	0.0	0.0018167026057834333	0.0	4.949882704201322	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.165676E-6	45.000004	2
GCGATAT	95	0.0	45.0	9
CGTTTTT	16885	0.0	43.85401	1
GTTTTTA	11210	0.0	42.95272	2
ATTGCGA	125	0.0	41.4	2
GAGCGAT	1000	0.0	40.725002	7
CGTAAGA	105	0.0	40.714283	2
ACGTTAG	50	1.0822987E-9	40.5	1
TATTTTT	12545	0.0	39.475887	7
TAACGAG	160	0.0	39.375004	3
TTATTTT	12580	0.0	39.34817	6
ACGACGG	230	0.0	39.130436	27
AGCGATA	250	0.0	38.7	8
TTTTATT	12820	0.0	38.681747	4
TTTATTT	12875	0.0	38.56893	5
CGGTCTA	240	0.0	38.437504	31
AGACTTA	905	0.0	38.038677	8
ATAACGC	125	0.0	37.800003	11
TTTTTAT	13280	0.0	37.528236	3
CTCACGA	240	0.0	37.500004	24
>>END_MODULE