FastQCFastQC Report
Thu 26 May 2016
SRR1546878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546878_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1230833
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134111.0895872957582386No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73380.5961816103403142No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG23340.18962767491609342No Hit
GAATCTGACTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC22840.1855653853934693No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC14810.12032501566012611No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12320.10009481383745804No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC207.0334825E-445.011
CTACCGT207.0334825E-445.029
TAATGCG207.0334825E-445.01
CGTTTTT104350.043.339721
TGTACGA1100.042.9545482
GTTTTTA69650.042.2864342
CGGTCTA2200.041.9318231
TCGATAG556.184564E-1140.9090921
GAGCGAT10150.040.7881777
TATACGG501.0822987E-940.5000042
CGTTAGA850.039.7058832
CGAGAAT1200.039.3750046
TATTTTT80250.038.8878487
AGGGGAA64600.038.869978
AGCGATC1800.038.758
CGACGGT2500.038.728
ATAACGC700.038.57142611
TAATCGG356.2486506E-638.5714262
TTTTATT81200.038.460594
TTATTTT81100.038.452536